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  4. Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

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  • 中文名称：
    金黄色酿脓葡萄球菌hly重组蛋白
  • 货号：
    CSB-YP639324FLF
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP639324FLF could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain NCTC 8325) hly.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP639324FLF could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain NCTC 8325) hly.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    hly
  • Uniprot No.：
  • 别名：
    hly; hla; SAOUHSC_01121Alpha-hemolysin; Alpha-HL; Alpha-toxin
  • 种属：
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    35.3kDa
  • 表达区域：
    27-319aa
  • 氨基酸序列
    ADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Recombinant full-length of Mature Staphylococcus aureus Alpha-hemolysin (hly) Protein (27-319 amino acids) with a 6xHis tag at the C-terminus was expressed in the yeast. SDS-PAGE analysis of the purified hly protein gave one band at 34–40 kDa of the target molecule. The purity of this hly protein is greater than 90%. The identification of the hly protein was validated by the LC-MS/MS Analysis. The delivery time is relatively short because the hly protein is in-stock.

    Alpha-hemolysin (hly), also known as hla, is a single component toxin generated by Staphylococcus aureus, a potential human causative agent that can cause various acute and chronic infections. Hly plays an important role in the pathogenesis of Staphylococcus aureus. The interaction between hly and the specific cell receptor ADAM10 forms cylindrical transmembrane heptameric pores. The resulting pores allow the influx and efflux of ions and small molecules, ultimately leading to disruption of ionic equilibrium, osmotic expansion, and cell death.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Inhibits host neutrophil chemotaxis to the lesion region (Probable). Heptamer oligomerization and pore formation is required for lytic activity.
  • 基因功能参考文献：
    1. We now show that ADAM10 is critical for alpha-hemolysin-mediated activation of the NLRP3 inflammasome in human monocytes as siRNA knockdown or chemical blockade of ADAM10-alpha-hemolysin interaction leads to diminished inflammasome activation and cell death by reducing the available ADAM10 on the cell surface. PMID: 27043625
    2. Staphylococcus aureus hemolysin A disrupts cell-matrix adhesions in human airway epithelial cells. PMID: 24918472
    3. Succeeded in preparing crystals of the heptameric form of alpha-hemolysin without any detergent but with 2-methyl-2,4-pentanediol (MPD), and determined its structure. PMID: 21280135
    4. Staphylococcus aureus alpha-hemolysin activates the NLRP3-inflammasome in human and mouse monocytic cells PMID: 19826485
    5. The wide vestibule leading into the alpha-hemolysin pore helps to orient negatively charged synthetic alpha-helical peptides for translocation. PMID: 16866363

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Aerolysin family
  • 数据库链接：

    KEGG: sao:SAOUHSC_01121

    STRING: 93061.SAOUHSC_01121