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  4. Recombinant Saccharomyces cerevisiae tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRM61

Recombinant Saccharomyces cerevisiae tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRM61

  • 货号：
    CSB-YP344421SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP344421SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP344421SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP344421SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP344421SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GCD14
  • Uniprot No.：
  • 别名：
    GCD14; TRM61; YJL125C; J0710; tRNA; adenine(58)-N(1))-methyltransferase catalytic subunit TRM61; EC 2.1.1.220; General control non-derepressible protein 14; Protein GCD14; tRNA(m1A58)-methyltransferase subunit TRM61; tRNA(m1A58)MTase subunit TRM61
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-383
  • 氨基酸序列
    MSTNCFSGYK DLIKEGDLTL IWVSRDNIKP VRMHSEEVFN TRYGSFPHKD IIGKPYGSQI AIRTKGSNKF AFVHVLQPTP ELWTLSLPHR TQIVYTPDSS YIMQRLNCSP HSRVIEAGTG SGSFSHAFAR SVGHLFSFEF HHIRYEQALE EFKEHGLIDD NVTITHRDVC QGGFLIKKGD TTSYEFGNNE TAASLNANVV FLDLPAPWDA IPHLDSVISV DEKVGLCCFS PCIEQVDKTL DVLEKYGWTD VEMVEIQGRQ YESRRQMVRS LNDALERLRD IKRHKLQGVE RRKRMFNNTI DSNDEKVGKR NEDGVPLTEK AKFNPFGKGS RIKEGDSNYK WKEVTKMEAE IKSHTSYLTF AFKVVNRSRD DEKVNEILRS TEK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency.
  • 基因功能参考文献：
    1. Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61 has been reported. PMID: 27582183
    2. X-ray diffraction analysis of the TRM6-TRM61 complex were performed in this study. The crystals diffracted to 2.80 A resolution and belonged to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 139.14, c = 101.62 A PMID: 22102254
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, TRM61 family
  • 数据库链接：

    KEGG: sce:YJL125C

    STRING: 4932.YJL125C