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  4. Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 4 (VPS4)

Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 4 (VPS4)

  • 中文名称：
    酿酒酵母VPS4重组蛋白
  • 货号：
    CSB-YP346411SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母VPS4重组蛋白
  • 货号：
    CSB-EP346411SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母VPS4重组蛋白
  • 货号：
    CSB-EP346411SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母VPS4重组蛋白
  • 货号：
    CSB-BP346411SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母VPS4重组蛋白
  • 货号：
    CSB-MP346411SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VPS4
  • Uniprot No.：
  • 别名：
    VPS4; CSC1; DID6; END13; GRD13; VPL4; VPT10; YPR173C; P9705.10Vacuolar protein sorting-associated protein 4; DOA4-independent degradation protein 6; Protein END13; Vacuolar protein-targeting protein 10
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-437
  • 氨基酸序列
    MSTGDFLTKG IELVQKAIDL DTATQYEEAY TAYYNGLDYL MLALKYEKNP KSKDLIRAKF TEYLNRAEQL KKHLESEEAN AAKKSPSAGS GSNGGNKKIS QEEGEDNGGE DNKKLRGALS SAILSEKPNV KWEDVAGLEG AKEALKEAVI LPVKFPHLFK GNRKPTSGIL LYGPPGTGKS YLAKAVATEA NSTFFSVSSS DLVSKWMGES EKLVKQLFAM ARENKPSIIF IDEVDALTGT RGEGESEASR RIKTELLVQM NGVGNDSQGV LVLGATNIPW QLDSAIRRRF ERRIYIPLPD LAARTTMFEI NVGDTPCVLT KEDYRTLGAM TEGYSGSDIA VVVKDALMQP IRKIQSATHF KDVSTEDDET RKLTPCSPGD DGAIEMSWTD IEADELKEPD LTIKDFLKAI KSTRPTVNED DLLKQEQFTR DFGQEGN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex.
  • 基因功能参考文献：
    1. The s now report a 3.2 A structure of Vps4 bound to an ESCRT-III peptide substrate. The new structure reveals that the peptide approximates a beta-strand conformation whose helical symmetry matches that of the five Vps4 subunits it contacts directly. PMID: 29165244
    2. The s propose a model in which multiple Vps4 hexamers (four or more) draw together several ESCRT-III filaments. This process induces cargo crowding and inward membrane buckling, followed by constriction of the nascent bud neck and ultimately intraluminal vesicle generation by vesicle fission. PMID: 29019322
    3. These data support a mechanism in which Vps4 disassembles its substrates by completely unfolding them and threading them through the central pore. PMID: 25938660
    4. Data implicate the ESCRT machinery in preventing the formation of nonfunctional nuclear pore complexes (NPCs); s propose a model in which ESCRT-III/Vps4 helps recognize and clear defective NPC assembly intermediates before a step in which they are committed to maturing into a malformed NPC destined for the storage of improperly assembled nuclear pore complexes compar PMID: 25303532
    5. Vfa1 is a novel positive regulator of Vps4 function PMID: 24567329
    6. analysis of the Vps4 AAA ATPase linker region, which plays a regulatory role in assembly and activity PMID: 23913684
    7. Results identify distinct spatiotemporal roles for Vps4-regulating proteins through examinations of subcellular localization and endosome morphology. PMID: 20089837
    8. Results present a systematic in vivo analysis of the Vps4 interaction network. PMID: 20110351
    9. Assembly of a Vps4p oligomeric complex with full ATPase activity that interacts with Vta1p is essential for normal endosome function. PMID: 16704411
    10. We determined the crystal structure of the Vps2 C terminus in a complex with the Vps4 MIT domain, explaining the basis for selective ESCRT-III recognition PMID: 17928861
    11. Vps4 functions in the MVB pathway via a highly conserved mechanism supported by similar protein-protein interactions during its ATPase reaction cycle PMID: 17949747
    12. The second region of homology (SRH) function of Vps4 requires an intact C-terminal helix and is important for assembly and intersubunit catalysis in AAA ATPases. PMID: 18266866
    13. Vps4p dimers form two distinct heptameric rings and accommodate AAA cassettes in a head-to-head--not in a head-to-tail-fashion as in class II AAA-ATPases PMID: 18272179
    14. ATP hydrolysis would eliminate this interaction and subsequent nucleotide release causes the domains to rotate, which together lead to the disassembly of the SKD1 oligomer PMID: 18796009
    15. Mutational analyses demonstrate that pore loop 2 residues Arg241 and Arg251 are required for efficient HIV-1 budding, thereby supporting a role for this "arginine collar" in Vps4 function. PMID: 18929572

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  • 亚细胞定位：
    Endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    AAA ATPase family
  • 数据库链接：

    KEGG: sce:YPR173C

    STRING: 4932.YPR173C