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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase ENV7 (ENV7)

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase ENV7 (ENV7)

  • 中文名称：
    酿酒酵母ENV7重组蛋白
  • 货号：
    CSB-YP604890SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母ENV7重组蛋白
  • 货号：
    CSB-EP604890SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母ENV7重组蛋白
  • 货号：
    CSB-EP604890SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母ENV7重组蛋白
  • 货号：
    CSB-BP604890SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母ENV7重组蛋白
  • 货号：
    CSB-MP604890SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ENV7
  • Uniprot No.：
  • 别名：
    ENV7; YPL236CSerine/threonine-protein kinase ENV7; EC 2.7.11.1; Late endosome and vacuole interface protein 7
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-364
  • 氨基酸序列
    MISIVLELFQ NLCCCRGFSD ATIRVNDKRY RIQRLLGEGG MSFVYLVQLS KNSLIIDNGI ATPELYALKK IICPSVESIS NGMREIENYK RFQSPYVIKS IDSQVMQEKD GSKTIYIVLP YYSLGSLQDS INRRLLEGTF VSEAECVRIM LGVTRGLLCL HDPASRQDNA TSRVNVDAVS MTYSDETAML LEDTPLEMDM LSSNSAGSIA YAHRDITPSN ILFSSDGLPV IGDLGSCSQA DITIENRHQL SELQEWVNDN CTLPYTPPEL LNLKLNQVLS SKVDIWSLGC TFYTLMFGIS PFEREEQIHG ASLTYAINTG KYSFPRNSRF SEGLLSVIKK CIQVDPIQRP TTSQLLNLLQ DLDT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in vacuolar processing and morphology.
  • 基因功能参考文献：
    1. These results establish negative genetic interactions between ENV7 and YCK3 and suggest cooperative roles for the two conserved genes in regulation of membrane dynamics. PMID: 24345185
    2. palmitoylation at the N-terminal cysteines of Env7 directs not only its membrane association but also its stability, phosphorylation, and cellular function. PMID: 24610781
    3. This study reports that yeast Env7 is a novel palmitoylated protein kinase ortholog that negatively regulates vacuolar membrane fusion. PMID: 23166297
  • 亚细胞定位：
    Vacuole membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: sce:YPL236C

    STRING: 4932.YPL236C