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  4. Recombinant Saccharomyces cerevisiae Mitochondrial import receptor subunit TOM20 (TOM20), partial

Recombinant Saccharomyces cerevisiae Mitochondrial import receptor subunit TOM20 (TOM20), partial

  • 中文名称：
    酿酒酵母TOM20重组蛋白
  • 货号：
    CSB-YP327402SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母TOM20重组蛋白
  • 货号：
    CSB-EP327402SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母TOM20重组蛋白
  • 货号：
    CSB-EP327402SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母TOM20重组蛋白
  • 货号：
    CSB-BP327402SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母TOM20重组蛋白
  • 货号：
    CSB-MP327402SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TOM20
  • Uniprot No.：
  • 别名：
    TOM20; MAS20; MOM19; YGR082W; Mitochondrial import receptor subunit TOM20; Mitochondrial 20 kDa outer membrane protein; Protein MAS20; Translocase of outer membrane 20 kDa subunit
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
  • 基因功能参考文献：
    1. Pex3p was targeted to mitochondria by fusion with the mitochondrial targeting signal of Tom20p. PMID: 20655617
    2. Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency. PMID: 21173275
    3. Our data reveal a large-scale mRNA association mode that involves interaction of Tom20p with the translated mitochondrial targeting sequence and may be assisted by Puf3p. PMID: 19858288
    4. Data Show that Tom20 are anchored in the outer membrane by a single transmembrane alpha-helix, located at the N terminus of Tom20 (signal-anchored) and that Mim1 is required for efficient membrane insertion and assembly of Tom20. PMID: 17974559
    5. Tom20 and Tom22 are apparently involved in the same step or sequential steps along the same pathway of targeting signal recognition in import PMID: 18063580
    6. These data show that the homo-oligomerization of Mim1 allows it to fulfil its function in promoting the integration of Tom20 into the mitochondrial outer membrane. PMID: 18177669
    7. Study shows that the transmembrane segment of Tom20 contains critical residues essential for docking the Tom20 receptor into its correct environment within the TOM complex; the docking reaction is catalyzed by the unique assembly factor Mim1/Tom13. PMID: 18187149

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass membrane protein.
  • 蛋白家族：
    Tom20 family
  • 数据库链接：

    KEGG: sce:YGR082W

    STRING: 4932.YGR082W