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  4. Recombinant Rat Glucose-6-phosphatase (G6pc), partial

Recombinant Rat Glucose-6-phosphatase (G6pc), partial

  • 中文名称：
    大鼠G6pc重组蛋白
  • 货号：
    CSB-YP009118RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠G6pc重组蛋白
  • 货号：
    CSB-EP009118RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠G6pc重组蛋白
  • 货号：
    CSB-EP009118RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠G6pc重组蛋白
  • 货号：
    CSB-BP009118RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠G6pc重组蛋白
  • 货号：
    CSB-MP009118RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    G6pc; G6ptGlucose-6-phosphatase; G-6-Pase; G6Pase; EC 3.1.3.9
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
  • 基因功能参考文献：
    1. we demonstrate an essential role for fructose 2,6-bisphosphate in the induction of glucose-6-phosphatase and other ChREBP target genes PMID: 22214556
    2. An increase in G6Pase activity was observed in liver microsomes from rats starved overnight, which presented a ~15% decrease in hepatic Mg(2+) content. PMID: 21402051
    3. Calorie restriction can inhibit the expressions of FoxO1, PEPCK and G-6-P, strengthen insulin signal conduction, suppress gluconeogenesis and thus regulate glycometabolism. PMID: 18304430
    4. promoter activity suppressed by polyunsaturated fatty acyl coenzyme a PMID: 11864989
    5. liver G-6-Pase activated in response to insulin-induced hypoglycemia or epinephrine infusion PMID: 11882511
    6. The effect of DYRK on the G6Pase promoter activity was produced by the isoforms DYRK1A and DYRK1B, but not by DYRK2 suggesting that DYRK1 is a specific co-activator of FKHR, independent of its kinase activity. PMID: 12507516
    7. kinetic properties of the glucose 6-phosphatase of liver microsomes in adjuvant arthritis PMID: 12757934
    8. dyrk1B is a Rho-induced kinase active in skeletal muscle differentiation PMID: 12902328
    9. glucose-6-phosphatase gene expression in cultured hepatocytes and H4IIE cells is regulated by short-chain fatty acids and HNF-4alpha PMID: 12915406
    10. G6pc expression was functionally silenced by adenovirus-mediated delivery of short hairpin RNA. PMID: 14759518
    11. Fructose levels affect hepatic glucose-6-phosphatase catalytic subunit mRNA. PMID: 14988444
    12. glucose 6-phosphatase has a role in the small intestine in the control of glucose homeostasis [review] PMID: 15302872
    13. the cAMP responsiveness of the Glc6Pase promoter requires a tight cooperation between a proximal and a distal region, which depends on the presence of several HNF4alpha-, C/EBP-, and CREB-binding sites PMID: 15388792
    14. These observations, together with the absence of a conserved LXR-binding element within the G6Pase promoter, suggest an indirect inhibition of G6Pase gene expression by liver X receptors. PMID: 16256938
    15. Multiple, distinct cis-regulatory promoter elements are involved in the glucose response of the rat G-6-Pase gene. PMID: 17106062
    16. G6pc is down-regulated during liver gluconeogenesis. PMID: 17158265
    17. A novel chimeric promoter, consisting of a 1.6 kb glucose 6-phosphatase promoter and a segment of the regulatory element derived from the L-type pyruvate kinase promoter, was designed to provide strong and tight control of insulin expression in liver. PMID: 17160355
    18. The co-expression of glucose 6-phosphatase and cytosolic phosphoenolpyruvate carboxykinase was studied in the digestive system and the kidney of the rat. PMID: 17211624
    19. The effect, which is lost when G6P has readily access to the ER lumen, requires physiological endoplasmic reticulum Mg(2+) content. PMID: 17931592
    20. The lower sensitivity of the liver nuclear envelope G-6-Pase to N-ethylmaleimide suggests that the catalytic subunit of this system has some differences from the microsomal isoform. PMID: 18717264

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Glucose-6-phosphatase family
  • 数据库链接：

    KEGG: rno:25634

    UniGene: Rn.10992