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  4. Recombinant Rat Diacylglycerol kinase alpha (Dgka)

Recombinant Rat Diacylglycerol kinase alpha (Dgka)

  • 中文名称：
    大鼠Dgka重组蛋白
  • 货号：
    CSB-YP006832RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Dgka重组蛋白
  • 货号：
    CSB-EP006832RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Dgka重组蛋白
  • 货号：
    CSB-EP006832RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Dgka重组蛋白
  • 货号：
    CSB-BP006832RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Dgka重组蛋白
  • 货号：
    CSB-MP006832RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dgka; Dagk; Dagk1; Diacylglycerol kinase alpha; DAG kinase alpha; EC 2.7.1.107; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-727
  • 氨基酸序列
    MAKDKGLISP EDFAQLQKYI DYSTKSVSDV LKVFEMNKYC QGDEIGYLGF EQFLKMYLEV EEVPHHLCWT LFWSFHSSQD LDEETESKAN VICLSDVYCY FTLLEGGSPE DKLEFTFKLY DMDRNGILDS TEVEKIILQM MRVAEYLDWD VSELRPILQE MMKEMDRDGS GCVSLAEWVR AGATTVPLLV LLGIDMTMKD DGHHIWRPKR FSRPVYCNLC ELSIGLGKQG LSCNLCKYIV HDHCAMKAQP CEVSTYAKSR KDIGVQPHVW VRGGCHSGRC DRCQKKIRTY HSLTGLHCVW CHLEIHDDCL QAVGPECDCG LLRDHILPPC SIYPRVLVSG QECKQKTTDV TSLCTPEAFR IEPVSNTHPL LVFINPKSGG KQGQSVLWKF QYILNPRQVF NLKDGPEPGL RFFKDVPQFR VLVCGGDGTV GWILETIDKA NFPIVPPVAV LPLGTGNDLA RCLRWGRGYE GENLRKILKD IEISKVVYLD RWLLEVIPQQ NGEKSDPVPS QIINNYFSIG VDASIAHRFH LMREKYPEKF NSRMKNKLWY LEFATSESIF STCKKLEESV TVEICGKLLD LSDLSLEGIA VLNIPSMHGG SNLWGDTKRP HGDTCGINQA LGSVAKIITD PDILKTCVPD MSDKRLEVVG IEGVIEMGQI YTRLKSAGHR LAKCSEITFQ TTKTLPMQVD GEPWMQAPCT IKITHKNQMP MLMGPAPSSS NFFGFWS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.
  • 基因功能参考文献：
    1. The DGKalpha localize predominantly to the nucleus of ganglion cells. PMID: 23467923
  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Eukaryotic diacylglycerol kinase family
  • 组织特异性：
    Lymphocytes and oligodendroglial cells.
  • 数据库链接：

    KEGG: rno:140866

    STRING: 10116.ENSRNOP00000038628

    UniGene: Rn.1840