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  4. Recombinant Rat Beta-nerve growth factor (Ngf)

Recombinant Rat Beta-nerve growth factor (Ngf)

Unavailable
  • 中文名称：
    大鼠Ngf重组蛋白
  • 货号：
    CSB-YP015779RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ngf; Ngfb; Beta-nerve growth factor; Beta-NGF
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    15.4kDa
  • 表达区域：
    122-241aa
  • 氨基酸序列
    SSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLGEVNINNSVFKQYFFETKCRAPNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDDKQAAWRFIRIDTACVCVLSRKAARRG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant Rat Ngf is a yeast-expressed (122-241aa) protein with N-terminal 6xHis tag. The purity is 90%+ measured by SDS-PAGE. The highly developed genetic system, ease of use, reduced time input, and costs have made Pichia Pastoris an attractive organism for the expression and production of recombinant proteins. So we choose the Yeast system to express this recombinant Ngf protein, which is able to carry specifically designed plasmids, and the plasmid used consists of restriction sites that can be used to insert the gene sequence of interest. Transformation of yeasts with the plasmid produces the desired protein and can be appropriately scaled up.

    Ngf is a neurotrophin that regulates the development and survival of neurons, particularly sympathetic and sensory neurons that transmit pain, temperature, and touch sensations. It also functions as an ovulation-inducing factor (OIF), which is found in the seminal plasma (SP) of some reflex ovulating species such as camelids or rabbits. Ngf is expressed in spermatogenic cells and has testosterone-downregulated low-affinity receptors on Sertoli cells implying a paracrine role in the modulation of spermatogenesis.

     

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form.
  • 基因功能参考文献：
    1. proinflammatory cytokines suppressed the TGFbetamediated expression of NGF in PDLderived fibroblasts through the inactivation of TGFbetainduced Smad2/3 and p38 MAPK signaling. PMID: 29901090
    2. Impairments in the extracellular maturation of proNGF are sufficient to cause a somatodendritic retrograde degeneration o fdegeneration of basal forebrain cholinergic neurons. PMID: 29609077
    3. McGill APP transgenic rats exhibit differential dysregulation in NGF and BDNF neurotrophins. At early stages, intraneuronal Abeta leads to significant reductions in BDNF mRNA expression, which correlate with learning and memory deficits. At later stages, deficits in the NGF metabolic pathway could explain the paradoxical upregulation of proNGF in the absence of changes in NGF mRNA. PMID: 28865749
    4. p75NTR is sufficient to mediate the modulation of myogenic cells differentiation by NGF in terms of structural, metabolic and functional changes. PMID: 29202822
    5. NGF protects GECs against IND-induced injury. Mitochondria are major targets of both INDO-induced injury and NGF afforded protection of GECs. TrkA expression in the mitochondria of GECs indicates that the protection afforded by NGF is partly mediated by its direct action on mitochondria. NGF prevents MMP depolarization and increases expression of IGF-1 protein in GECs. PMID: 28843696
    6. The results suggest that NGF is a prominent hyperalgesic mediator in the trigeminal system and plays a role in trigeminal neuropathic pain PMID: 27392124
    7. Early and late behavioral changes in sciatic nerve injury may be modulated by nerve growth factor and substance P in rats PMID: 28685530
    8. A higher level of nerve growth factor in the early days post-transvaginal mesh implantation is associated with a shorter voiding interval and a smaller bladder capacity, which represents abnormal lower urinary tract symptoms following transvaginal mesh implantation. PMID: 27762470
    9. Findings might aid in identifying effective treatment windows for nerve growth factor (NGF)-based strategies to counteract retinal and/or optic-nerve degeneration. PMID: 28067793
    10. The aim of our study was to investigate the influence of two mild stressors, acute and chronic exposure to forced swim (FS) or high-light open field (HL-OF), on neurons containing NGF. PMID: 27978417
    11. This work sheds light on the role of miR-132 in bladder overactivity, bladder hypertrophy, NGF signaling and expression of inflammatory mediators. Findings demonstrate that aberrant expression of NGF and miR-132 is involved in voiding dysfunctions. PMID: 27789288
    12. Data indicate the effects of vitamin B12 and omega-3 fatty acid supplementation across three consecutive generations on brain neurotrophins like brain derived neurotrophic factor (BDNF); nerve growth factor (NGF) and cognitive performance in the third generation male offspring. PMID: 27569259
    13. This study demonstrated that Age-dependent changes in the density of NGF-ir neurons in stressed rats are probably caused by ageing processes and they may point to dysregulation of excitatory control exerted by the amygdala. PMID: 26724365
    14. Data indicate that nerve growth factor precursors and nerve growth factor (ProNGF and NGF) and the downstream effector proteins play an important role in the growth, differentiation, and apoptosis of oligodendrocyte (OLG). PMID: 26681653
    15. NGF in the facet joint contributes to the development of injury-induced joint pain. Localized blocking of NGF signaling in the joint may provide potential treatment for joint pain. PMID: 26521746
    16. This study deminstrated that elevated NGF after peripheral nerve injury induces neurite sprouting and the formation of synapse-like structures within the contralateral DRG, leading to the development of chronic mirror-image pain. PMID: 26121254
    17. Results suggest the induction of NGF represents an adaptive response against immune-mediated neuroinflammation in the dorsal root ganglia and spinal cord that likely contributes to the experimental autoimmune encephalomyelitis attenuation PMID: 25801841
    18. elevation of plasma norepinephrine in neonatal colonic inflammation induces NGF expression in the gastric fundus. PMID: 26608656
    19. NGF acts directly on photoreceptors survival and prevents photoreceptor degeneration. PMID: 25897972
    20. Identify nerve growth factor as a binding partner for MOG and demonstrate that this interaction is capable of sequestering NGF from TrkA-expressing neurons to modulate axon growth and survival. PMID: 26347141
    21. Let-7 microRNAs regulate peripheral nerve regeneration by targeting nerve growth factor. PMID: 25394845
    22. Ginger extract has a synaptogenic effect via NGF-induced ERK/CREB activation, resulting in memory enhancement. PMID: 25049196
    23. Chronic heart failure impairs the expression of nerve growth factor in the sympathetic and sensory nerves. PMID: 24913185
    24. Vibration significantly increases total-NGF mRNA in interverterbal discs. Protein expression of BDNF increases by nearly 10-fold, especially in the inner annulus fibrosus and nucleus pulposus. PMID: 24921856
    25. NGF exhibits anti-oxidative and hepatoprotective effects and is suggested to be therapeutically applicable in treating cholestatic liver diseases. PMID: 25397406
    26. Activation of microtubule dynamics increases neuronal growth via the NGF- and Galpha s-mediated signaling pathways. PMID: 25691569
    27. NGF was increased 12, 24, and 48 hours in hippocampus after sepsis induction PMID: 24978930
    28. Findings revealed that NGF may play a pivotal role of anti-apoptosis in spinal cord neurons through retrograde transport of NF-kappaB in Schwann cells following sciatic nerve injury in rats PMID: 24620979
    29. Maternal separation decreased NGF levels in the hippocampus which was reversed with methamphetamine. PMID: 24407463
    30. the level of NGF expression did not display a meaningful change throughout the immobilization period PMID: 24154957
    31. Spinal nerve afferents are important in sustaining and up-regulating the expression of NGF in the sensory neurons innervating the heart in acute myocardial infarction. PMID: 24508054
    32. NGF played an important role in the activation of Akt and subsequent up-regulation of BDNF in the sensory neurons in visceral inflammation such as cystitis. PMID: 24303055
    33. The temporal mismatch in behaviour, skin [NGF] and phenotypic changes in sensory nerve fibres indicate that increased [NGF] does not cause hyperalgesia after partial mental nerve injury. PMID: 24380503
    34. Using gene expression as a functional read-out, our data demonstrate that the relative availability of NGF and proNGF in vivo might modulate the biological outcome of these ligands. PMID: 24713110
    35. The synergistic effects of grafting both NGF and GRGD ligands to PCL-CS scaffolds on the growth and differentiation of PC12 cells provide a new biomaterial for neural tissue engineering. PMID: 23468336
    36. Denervation causes age-dependent increases in NGF in the VP, which is a potential mechanism by which the autonomic nervous system may regulate prostate growth and lead to BPH/LUTS PMID: 23872662
    37. proBDNF accumulated in the hippocampus of aged rats. PMID: 23255148
    38. The effects of acute and chronic administration of branched-chain amino acids on protein levels and mRNA expression of nerve growth factor, was investigated. PMID: 23559405
    39. Suggest that nerve growth factor, which is elevated in the failing human heart, causes hypertrophy of neurons in cardiac ganglia. PMID: 23959444
    40. Hyperosmolarity induces apoptosis of pHCECs by activating JNK signaling. PMID: 24327613
    41. Environmental enrichment improves memory and increases nerve growth factor (NGF) concentration in the dentate gyrus. PMID: 23460346
    42. NGF contributes to the repair of nerve tissue and improves pain-related behavior after inferior alveolar nerve injury. PMID: 23190308
    43. Long-term casting induced heat hyperalgesia, and up-regulation and phenotypic change of NGF in dorsal root ganglia PMID: 23234417
    44. Data indicate that nerve growth factor (NGF) mRNA levels changed significantly during Leydig-cell regeneration in vivo. PMID: 23743199
    45. Upregulation of NGF in the amygdala promotes behavior of opioid reward and increases sensitivity to subsequent opioids. PMID: 22871918
    46. This study suggests that application of hADSCs with NGF-hydrogel on the CN might be a promising treatment for postprostatectomy ED. PMID: 22834730
    47. NGF increased expression of ASIC3 in DRG neurons. PMID: 22744968
    48. [REVIEW] Neuropathies and clinical presentations result from disruption of NGF signalling in hereditary sensory autonomic neuropathies (HSAN) type IV and HSAN type V. PMID: 23157347
    49. NGF gene expression is elevated during the whole course of cerebral concussion, especially in the early phase. PMID: 12857442
    50. There were much more Ngf-immunoreactive nerve cells expressed in the dorsal horn in sciatic nerve cryoneurolysis rats with hyperalgesia. PMID: 22580175

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  • 亚细胞定位：
    Secreted. Endosome lumen.
  • 蛋白家族：
    NGF-beta family
  • 组织特异性：
    Detected in the granule and pyramidal cell layer in the hippocampus.
  • 数据库链接：

    KEGG: rno:310738

    STRING: 10116.ENSRNOP00000022200

    UniGene: Rn.22168