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  4. Recombinant Mouse cGMP-specific 3,5-cyclic phosphodiesterase (Pde5a), partial

Recombinant Mouse cGMP-specific 3,5-cyclic phosphodiesterase (Pde5a), partial

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  • 中文名称：
    小鼠Pde5a重组蛋白
  • 货号：
    CSB-EP807017MO
  • 规格：
    ￥1836
  • 促销：
    促销单品低至599元起
  • 图片：
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP807017MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pde5a.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP807017MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pde5a.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pde5a; Pde5; cGMP-specific 3',5'-cyclic phosphodiesterase; EC 3.1.4.35; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    26.1 kDa
  • 表达区域：
    154-320aa
  • 氨基酸序列
    DVTALCHKIFLHIHGLISADRYSLFLVCEDSSKDKFLISRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAFGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.
  • 基因功能参考文献：
    1. analysis of the mechanisms involved revealed the induction of bone morphogenetic protein-7 (BMP7) expression, a critical regulator of angiogenesis and kidney homeostasis, through a PDE5i-dependent downregulation of miR-22. In conclusion PDE5i slows the progression of Diabetic Nephropathy (DN) in mice, improving hemodynamic parameters and vessel integrity. PMID: 28294194
    2. the activation of PDE5 is among the mechanisms contributing to the cGMP decrease, these results suggest that another cGMP phosphodiesterase is also activated by LH signaling. PMID: 29341896
    3. Report expression of Pde5a1/2/3 in cardiac myocytes and suggest role in induction of cardiac hypertrophy. PMID: 28247930
    4. Upon S-nitrosylation, PDE5 exhibits reduced activity and degradation via the ubiquitin-proteasome system. PMID: 26093192
    5. Short-term hemolysis is sufficient to establish a priapism phenotype and results in loss of erectile function. PDE5I treatment ameliorates priapism, in part, because of restored NO balance with decreased ROS generation and increased PDE5 activity. PMID: 26346631
    6. Inhibition of type 5 phosphodiesterase counteracts beta2-adrenergic signalling in beating cardiomyocytes. PMID: 25852085
    7. Addition of sildenafil, a phosphodiesterase 5 (PDE5) inhibitor, in the drinking water had a small effect in reducing myocyte hypertrophy in wild type mice and no effect in betaRM mice. PMID: 25139994
    8. findings reveal that excess adenosine-mediated ADORA2B signaling underlies reduced penile PDE activity by decreasing PDE5 gene expression in a HIF-1alpha-dependent manner PMID: 24614760
    9. Phosphodiesterase 5 attenuates the vasodilatory response in renovascular hypertension. PMID: 24260450
    10. Findings suggest that continuous, long-term treatment with PDE5 inhibitors reverses eNOS uncoupling in the sickle cell penis which restores endothelial NO synthesis. PMID: 23844149
    11. Data indicate that the phosphodiesterase 5 (PDE5) expression is equal in the splenocytes from both genders, but splenocytes from female mice possess higher basal level of cGMP compared to the male ones. PMID: 23911424
    12. Myocardial PDE5 expression is increased in the hearts of humans and mice with chronic pressure overload. PMID: 23527037
    13. Sildenafil-mediated PDE5 inhibition significantly reduces diaphragm respiratory muscle dysfunction and pathology in the mdx mouse model of Duchenne muscular dystrophy. PMID: 22653783
    14. these data strongly support a primary role of myocyte PDE5 regulation to myocardial pathobiology PMID: 20970280
    15. PDE5-inhibition blocks TRPC6 channel activation and associated Cn/NFAT activation signaling by PKG-dependent channel phosphorylation PMID: 19961855
    16. PDE5 inhibition enhances ischemia-induced angiogenesis with mobilization of endothelial progenitor cells through a protein kinase G-dependent HIF-1/vascular endothelial growth factor pathway. PMID: 20413734
    17. phosphorylation and functional suppression of TRPC6 underlie prevention of pathological hypertrophy by PDE5 inhibition. PMID: 20177073
    18. Myocardial oxidative stress increases PDE5 expression in the failing heart PMID: 20308615
    19. Data show that recombinant cyclic GMP-binding phosphodiesterase 5 (PDE5) is activated directly upon cGMP binding to the GAF A domain, and this effect does not require PDE5 phosphorylation. PMID: 12554648
    20. PDE5A has a novel regulatory role in hearts under adrenergic stimulation. PDE5A catabolic regulation is specific coupled with NOS3-derived cGMP attributable to protein subcellular localization and targeted synthetic/catabolic coupling. PMID: 15576651
    21. findings show a much greater role of PDE5A in hearts subjected to sustained pressure load and that PDE5A inhibition in this setting prevents and reverses cardiac chamber, cellular and molecular remodeling induced by this stimulus PMID: 15665834
    22. sildenafil inhibits PDE5 and preconditions adult cardiac myocytes against necrosis and apoptosis PMID: 15668244
    23. Priapism causes dysregulated PDE5A expression/activity suggesting that PDE5A dysregulation is a fundamental mechanism for priapism. PMID: 15668387
    24. Regulation of cardiac beta-adrenergic response by cGMP is specifically linked to a nitric oxide-synthesis/PDE-5-hydrolyzed pool signaling via protein kinase G. PMID: 17420342
    25. Inhibition by sildenafil ameliorates right ventricular hypertrophy and pulmonary fibrosis caused by intratracheal bleomycin. PMID: 17965319
    26. Upregulating the NO/cGMP pathway by PDE-5 inhibition during hypoxia reduces neuron apoptosis, regardless of HIF-1alpha, through an interaction involving ERK1/2 and p38. PMID: 18641057
    27. sildenafil activates a PKG-dependent novel signaling cascade that involves activation of ERK and inhibition of glycogen synthase kinase 3beta leading to cytoprotection PMID: 18723505
    28. Ten weeks after myocardial infarction, left ventricular end-systolic and end-diastolic volumes were larger in PDE5-transgenic than in wild-type mice. PMID: 19139381
    29. These data suggests a novel physiological role for PDE5 in restricting the effects of NOS3/sGC/PKG signaling pathway to modulating beta-AR stimulated I(Ca), while limiting effects on cardiac contraction. PMID: 19345227

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  • 蛋白家族：
    Cyclic nucleotide phosphodiesterase family
  • 数据库链接：

    KEGG: mmu:242202

    STRING: 10090.ENSMUSP00000069011

    UniGene: Mm.134911