Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse cAMP-dependent protein kinase catalytic subunit alpha (Prkaca)

Recombinant Mouse cAMP-dependent protein kinase catalytic subunit alpha (Prkaca)

  • 中文名称：
    小鼠Prkaca重组蛋白
  • 货号：
    CSB-YP018688MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Prkaca重组蛋白
  • 货号：
    CSB-EP018688MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Prkaca重组蛋白
  • 货号：
    CSB-EP018688MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Prkaca重组蛋白
  • 货号：
    CSB-BP018688MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Prkaca重组蛋白
  • 货号：
    CSB-MP018688MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkaca; Pkaca; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; EC 2.7.11.11
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-351
  • 氨基酸序列
    GNAAAAKKG SEQESVKEFL AKAKEDFLKK WETPSQNTAQ LDQFDRIKTL GTGSFGRVML VKHKESGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV MEYVAGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFTE F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in chondrogenesis by mediating phosphorylation of SOX9. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock.; Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
  • 基因功能参考文献：
    1. The cooperativity mechanism of Pkaca critically depends on the presence of water in two distinct, buried hydration sites. PMID: 29581285
    2. Study validates the DNAJB1-PRKACA fusion kinase as an oncogenic driver and candidate drug target for fibrolamellar hepatocellular carcinoma in mouse model in which tumorigenesis was significantly enhanced by genetic activation of beta-catenin. PMID: 29162699
    3. Protein kinase A signaling explains vasopressin-mediated regulation of membrane trafficking and gene transcription. PMID: 28973931
    4. Data suggest that the Girvan-Newman algorithm-based community maps of the kinase domain of cAMP-dependent protein kinase A (PKA) allow for a molecular explanation for the role of protein conformational entropy in its catalytic cycle. PMID: 28115705
    5. Acute versus chronic exposure to high fat diet leads to distinct regulation of PKA. PMID: 28420713
    6. Data suggest that the upregulation of mitochondrial respiratory chain proteins played a partial role in the protection of PKA/CREB signaling. PMID: 29038164
    7. HIF1a transcriptional activity is stimulated by Protein kinase A-dependent phosphorylation PMID: 27245613
    8. Generation of the Dnajb1-Prkaca fusion gene in wild-type mice to be sufficient to initiate formation of tumors that have many features of human fibrolamellar hepatocellular carcinonma. PMID: 28923495
    9. Data indicate a subpopulation of the CaV1.2 channel pore-forming subunit (alpha1C) within nanometer proximity of protein kinase A (PKA) at the sarcolemma of murine and human arterial myocytes. PMID: 28119464
    10. S1928A KI mice failed to induce long-term potentiation in response to prolonged theta-tetanus (PTT-LTP), a form of synaptic plasticity that requires Cav1.2 and enhancement of its activity by the beta2-adrenergic receptor (beta2AR)-cAMP-PKA cascade. PMID: 28119465
    11. study identifies a new role of Dual-AKAP1 in regulating mitochondrial trafficking through Miro-2, and supports a model in which PINK1 and mitochondrial PKA participate in a similar neuroprotective signaling pathway to maintain dendrite connectivity PMID: 28556983
    12. Data suggest that enzyme activation by cAMP involves highly stable conformation of Prkar1a as it binds to Prkaca; glycine residue, G235, appears to function as hinge in B/C helix conserved in Prkar1a; this "Flipback" conformation plays role in cAMP association to A domain of Prkar1a. (Prkar1a = cyclic AMP-dependent protein kinase RIalpha subunit; Prkaca = cyclic AMP-dependent protein kinase catalytic subunit) PMID: 28661131
    13. cAMP dependent protein kinase A (PKA) is a key intracellular factor mediating SHH signaling through regulation of GLI3 processing. PMID: 27720745
    14. this study shows illustrates for the first time the ability of protein kinase A to increase colony-stimulating factor 1 receptor DNA methylation resulting in macrophage maturation PMID: 27353657
    15. these results suggest that the activation of 5-HT1D receptors selectively enhanced IA via the Gbetagamma of the Go-protein, PKA, and the sequential B-Raf-dependent p38 MAPK signaling cascade. PMID: 27156838
    16. These data indicate that LPA increases CCN2 expression through the activation of PKC and PKA. Thus, the regulatory functions of the PKC and PKA pathways are implicated in the LPA-induced increase in CCN2 expression PMID: 26743816
    17. PKA phosphorylates the ATPase inhibitory factor 1 and inactivates its capacity to bind and inhibit the mitochondrial H(+)-ATP synthase. PMID: 26387949
    18. Data suggest PRKAR1A contains two structurally homologous cAMP-binding domains that exhibit marked differences in dynamic profiles in activation/inhibition of Prkaca; conservation of structure does not necessarily imply conservation of dynamics. PMID: 26618408
    19. Data (including data from studies in transgenic mice) suggest that constitutive activation of Prkaca in osteoblasts is sufficient to increase bone mass, favorably modify bone architecture, and improve mechanical properties of bone. PMID: 26488807
    20. Lin28a protects against DCM through PKA/ROCK2 dependent pathway. PMID: 26626075
    21. Findings show that cAMP acts via protein kinase A (PKA) to regulate multiple aspects of mitochondrial function and structure. PMID: 26203188
    22. acidosis in inflamed tissues may be a decisive factor to regulate switching of PKA and PKCepsilon dependence via proton-sensing G-protein-coupled receptors. PMID: 25933021
    23. Data suggest that investigation of cAMP-dependent protein kinase A (PKA) and its signaling in D2 receptor-expressing indirect pathway medium-sized spiny neurons (iMSNs) would better understand the mechanisms underlying psychiatric disorders. PMID: 26305972
    24. This study investigates the affinity between the protein kinase PKA and peptide substrates derived from kemptide using molecular dynamics simulations. PMID: 25275314
    25. Our results provide evidence that cSrc is activated downstream of PKA and that inhibition of its activity blocks the capacitation-induced hyperpolarization of the sperm plasma membrane . PMID: 26060254
    26. Upon ligand and pseudosubstrate binding, the hydrogen bond networks undergo extensive reorganization, revealing that the open-to-closed transitions require global rearrangements of the internal forces that stabilize the enzyme's fold. PMID: 26030372
    27. Collectively, our studies highlighted that the CuB-induced actin aggregation and cofilin-actin rod formation was mediated via the Ga13/RhoA/PKA/VASP pathway. PMID: 24691407
    28. Angiotensin II activates PKA and CaMKII via NOX2, which results in disturbed sodium and calcium currents (via PKA) and enhanced diastolic sarcoplasmic reticulum Calcium leakage. PMID: 25073061
    29. protein kinase A and perilipin 5 interact to regulate cardiac lipolysis PMID: 25418045
    30. Data indicate that Ppif protein cyclophilin D (CypD)-dependent protein kinase A (PKA)/cAMP regulatory-element-binding (CREB) signaling is responsible for amyloid beta (Abeta)-induced synaptic injury. PMID: 23507145
    31. The findings link the reduced levels of IGF-1 caused by fasting to PKA signaling and establish their crucial role in regulating hematopoietic stem cell protection, self-renewal, and regeneration. PMID: 24905167
    32. A dominant-negative Rad mutation causes arrhythmogenesis via PKA-mediated phosphorylation of RYR2 activity in the heart. PMID: 25193703
    33. PKA turnover by the REGgamma-proteasome modulates FoxO1 cellular activity and VEGF-induced angiogenesis. PMID: 24560667
    34. LKB1/AMPK and PKA control ABCB11 trafficking and polarization in hepatocytes. PMID: 24643070
    35. nitrite promotes mitochondrial fusion by increasing the profusion protein mitofusin 1 while concomitantly activating protein kinase A (PKA), which phosphorylates and inhibits the profission protein dynamin-related protein 1 (Drp1) PMID: 24556414
    36. Data indicate mutation of the beta1 adrenergic receptor (beta1AR) promotes sustained agonist-induced cAMP activity, protein kinase A (PKA) phosphorylation, and cardiac myocyte contraction response. PMID: 24713698
    37. Phosphorylation of beta1-AR by PKA stimulates active Ca(2+)influx through TRPV5. PMID: 24828496
    38. Data indicate that high protein kinase A (PKA) activity correlated negatively with migration velocity. PMID: 24842369
    39. The dynamic mitochondrial-nuclear shuttling of FKBP51 regulated by PKA may be key in fine-tuning the transcriptional control of glucocorticoid receptor target genes required for the acquisition of adipocyte phenotype. PMID: 24101724
    40. protein kinase A (PKA) inhibits potassium currents activated by protein kinase C (PKC) in freshly isolated aortic myocytes. PMID: 24086441
    41. Studied Ssanghwa-tang alpha-MSH-induced melanogensis and its mechanisms of action in murine B16F10 melanoma cells. Ssanghwa-tang efficiently inhibited c-AMP-induced melanin synthesis in B16F10 cells via suppression of PKA and p38 MAPK signaling pathways. PMID: 23981281
    42. These data imply that the protein kinase A and mTOR signaling cascades are important for the development of follicular thyroid carcinogenesis and may suggest new targets for therapeutic intervention. PMID: 24512487
    43. PKC and PKA regulate AChR dynamics at the neuromuscular junction of living mice. PMID: 24260568
    44. Chondrogenesis induced by PTH in mesenchymal progenitor cells is mediated by Runx1, which involves the activation of PKA. PMID: 24058535
    45. The new transcript variant encoding Calpha3 isoform has N-terminus that differs from Calpha1 and Calpha2 isoforms. PMID: 23456795
    46. Canonical betaAR-mediated signaling coupled to PKA activation results in phosphorylation of spinophilin, disrupting its interaction with alpha2AARs and accelerating alpha2AAR endocytic responses. PMID: 23965992
    47. Data indicate that TRPV4 activity and TRPV4 trafficking are under discrete but synergistic control of PKC- and PKA-dependent pathways. PMID: 23709216
    48. A PKA-mediated signaling pathway mediates GnRH activation of CREB at low-pulse frequencies, playing a significant role in the decoding of the hypothalamic GnRH signal to result in frequency-dependent FSHbeta activation. PMID: 23393127
    49. Data show that in skeletal muscle cells, oleic acid induces cAMP/PKA and SIRT1 Ser-434 phosphorylation and activity, causing PGC1alpha deacetylation and increased fatty acid oxidation. PMID: 23329830
    50. Use constitutively active PKA catalytic subunit to show that PKA/CREB signaling may be a very early regulator of endothelial and hematopoietic cell differentiation. PMID: 22267325

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Membrane; Lipid-anchor.; [Isoform 2]: Cell projection, cilium, flagellum. Cytoplasmic vesicle, secretory vesicle, acrosome.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
  • 组织特异性：
    Isoform 2 is sperm specific.
  • 数据库链接：

    KEGG: mmu:18747

    STRING: 10090.ENSMUSP00000005606

    UniGene: Mm.19111