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  4. Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 1 (Usp1)

Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 1 (Usp1)

  • 货号：
    CSB-YP805908MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP805908MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP805908MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP805908MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP805908MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Usp1; Ubiquitin carboxyl-terminal hydrolase 1; EC 3.4.19.12; Deubiquitinating enzyme 1; Ubiquitin thioesterase 1; Ubiquitin-specific-processing protease 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-784
  • 氨基酸序列
    MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQENEEKTS EYRGSEIDQV VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY FCPGFKTGVK HLFNIISRKK EALKDDSNQK DKGSCKEESL ASYELICSLQ SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGFLQHDA QEVLQCILGN IQETCQLLKK EEIKNLAELS GKVEEQSLQK EETGGITSTE IDSMRNTEDV KEQLPKGNWK RKSDSESSNV KKKVKLSRES QPLEENQRQT RSKRKAIGDT LEAAPKIIPK CVSESESAKP SQKKSKVKIN WLKPSTKQPS ILSKFCSLGK ITTNQRSKGQ PKEKEGDVEE DLEKYGSDHT ANGGPESPGS SVTPVDSSEA KSGNKGAEQI GFELVEKLFQ GQLVLRTRCL ECESLTERRE DFQDISVPVQ EDELSKVEES SEISPEPKTE MKTLRWAISQ FASVERIVGE DKYFCENCHH YTEAERSLLF DKMPEVITIH LKCFAASGLE FDCYGGGLSK INTPLLTPLK LSLEEWSTKP TNDSYGLFAV VMHSGITISS GHYTASVKVT DLNSLELDEG NFVVDQMCEL GKPEPLTEEQ ARGTAGNYDD EVSIRVGGNA QPSKVLNKKN VEGIGLLGGQ KSKADYELYN KASNPDKVVG TPFTDNRNSE TNDTTNGTHE SDRNKESSDQ TGVNMNGLEN KISYVVQSLK EYEGKWLLFD DSEVKVTEEK DFLNSLSPST SPTSTPYLLF YKKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
  • 基因功能参考文献：
    1. our results strongly suggest that Usp1 is involved in the regulation of centrosome duplication, at least in part via ID1, and Usp1 may exert its oncogenic activity, partially through inducing centrosome abnormality. PMID: 26822809
    2. Data suggest Usp1 down-regulation by autocleavage is critical for Usp1 to exert role in DNA interstrand crosslink repair; Usp1 role is de-ubiquitination of Fancd2 (Fanconi anemia complementation group D2) and Pcna (proliferating cell nuclear antigen). PMID: 26783108
    3. Homozygous Uaf1(-/-) embryos died at embryonic day 7.5 (E7.5). These mutant embryos were small and developmentally retarded. PMID: 24001775
    4. Autocleaved Usp1 remains a deubiquitylase because its fragments remain associated with Uaf1, an enhancer of Usp1 activity, until the C-terminal fragment is selectively destroyed by the Arg/N-end rule pathway. PMID: 23159736
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Peptidase C19 family
  • 数据库链接：

    KEGG: mmu:230484

    STRING: 10090.ENSMUSP00000030289

    UniGene: Mm.371692