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  4. Recombinant Mouse Transcription factor 7-like 2 (Tcf7l2)

Recombinant Mouse Transcription factor 7-like 2 (Tcf7l2)

  • 中文名称：
    小鼠Tcf7l2重组蛋白
  • 货号：
    CSB-YP846068MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tcf7l2重组蛋白
  • 货号：
    CSB-EP846068MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tcf7l2重组蛋白
  • 货号：
    CSB-EP846068MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tcf7l2重组蛋白
  • 货号：
    CSB-BP846068MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tcf7l2重组蛋白
  • 货号：
    CSB-MP846068MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Tcf7l2; Tcf4; Transcription factor 7-like 2; HMG box transcription factor 4; T-cell-specific transcription factor 4; T-cell factor 4; TCF-4; mTCF-4
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-459
  • 氨基酸序列
    MPQLNGGGGD DLGANDELIS FKDEGEQEEK NSENSSAERD LADVKSSLVN ESETNQDSSS DSEAERRPPP RSESFRDKSR ESLEEAAKRQ DGGLFKGPPY PGYPFIMIPD LTSPYLPNGS LSPTARTYLQ MKWPLLDVQA GSLQSRQTLK DARSPSPAHI VSNKVPVVQH PHHVHPLTPL ITYSNEHFTP GNPPPHLPAD VDPKTGIPRP PHPPDISPYY PLSPGTVGQI PHPLGWLVPQ QGQPVYPITT GGFRHPYPTA LTVNASMSRF PPHMVPPHHT LHTTGIPHPA IVTPTVKQES SQSDVGSLHS SKHQDSKKEE EKKKPHIKKP LNAFMLYMKE MRAKVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL YPGWSARDNY GKKKKRKRDK QPGETNEHSE CFLNPCLSLP PITDLSAPKK CRARFGLDQQ NNWCGPCSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif CCTTTGATC in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
  • 基因功能参考文献：
    1. This work identified Tcf7L2 as the principal transcription factor mediating canonical Wnt signalling in the embryonic neocortex. PMID: 29751817
    2. TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and primary adipocyte stem cells and that TCF7L2 expression is required for the regulation of Wnt signaling during adipogenesis. PMID: 29317436
    3. Data (including data from studies in transgenic mice) suggest that inactivation of Tcf7l2 in pancreatic pericytes is associated with impaired expression of genes required for pancreatic beta-cell function and maturity of isolated pancreatic islets. Transgenic mice in which Tcf7l2 is selectively inactivated in pancreatic pericytes exhibit glucose intolerance. PMID: 29246974
    4. TCF7L2 does not affect oligodendrocyte precursor cells during remyelination. PMID: 28656232
    5. Findings demonstrate an alpha cell-autonomous role for Tcf7l2 in the control of pancreatic glucagon secretion and the maintenance of alpha cell mass and function. PMID: 28343277
    6. Suggest transcription factor 7-like 2 is a possible regulator of glucagon-like peptide 1 receptor expression in endothelial/smooth muscle cells in diabetic mice. PMID: 28830217
    7. along with the elevation of miR-17-5p expression in mouse epididymal fat tissue in response to high fat diet consumption, allowed us to suggest that miR-17-5p is among central switches of adipogenic differentiation PMID: 28102847
    8. TCF7L2 mediates canonic Wnt/beta-catenin signaling and c-Myc upregulation during abnormal cardiac remodeling in heart failure and suppression of Wnt/beta-catenin to c-Myc axis can be explored for preventing and treating heart failure. PMID: 27301468
    9. These findings suggest a unique role for Tcf7l2 in generating distinct neuronal phenotypes from homogeneous progenitor population. PMID: 28219675
    10. Tcf7l2 may be involved in maintenance of stem/progenitor cells properties. PMID: 27281479
    11. results indicate that miR-181a-5p promotes 3T3-L1 preadipocyte differentiation and adipogenesis through regulating TGFbeta/Smad and Wnt signaling pathway by directly targeting Smad7 and Tcf7l2 PMID: 27742678
    12. Tcf7l2 protein levels decline upon initiation of endocrine differentiation in vivo, disclosing the downregulation of this factor in the developing endocrine compartment. PMID: 26771085
    13. Tcf7l2 plays a cell autonomous role in the control of beta cell function and mass, serving as an important regulator of gene expression and islet cell coordination PMID: 25355422
    14. Tcf7l2 is regulating proinsulin expression directly via Isl1, Ins1 and indirectly via MafA, NeuroD1 and Pdx1. PMID: 25015099
    15. Transcription factor 7-like 2 positively regulates neonatal and postnatal mouse oligodendrocyte differentiation during developmental myelination and remyelination in a manner independent of the Wnt/beta-catenin signaling pathway. PMID: 25810530
    16. TCF7L2 expressed in the pancreatic beta cells plays a crucial role in glucose metabolism through regulation of the beta cell mass. PMID: 24317852
    17. Marked expression of TCF7L2 in oligodendrocytes is restricted to a well defined time period during developmental myelination in human and mouse central nervous system tissue samples. PMID: 23977356
    18. 5-FU acutely induces the severe myelin degeneration in adolescence and disruption of TCF7L2/HDAC1/HDAC2 complex is at least partially involved in 5-FU-induced demyelination. PMID: 25178657
    19. Knockdown of TCF7L2 blocked the malondialdehyde-induced glucose-stimulated insulin secretion elevation. PMID: 24035868
    20. This is the first report to show that DIF-1 inhibits tumor growth in vivo, consistent with its in-vitro action, suggesting that this compound may have potential as a novel anti-tumor agent. PMID: 24670930
    21. The dominant-negative isoforms of TCF7L2 are predominant in the embryonic thalamus but disappear postnatally. PMID: 23152144
    22. an essential role for Tcf4 during homeostasis of the adult mouse intestine PMID: 22393260
    23. Data suggest that TCF7L2 and Wnt signaling control gut and brain proglucagon expression and glucose homeostasis; positive cross-talk between Wnt and glucagon-like peptide 1/cAMP signaling appears to regulate brain metabolism. PMID: 22966074
    24. robust TCF7L2 expression was found in the tectal and tegmental structures of the superior and inferior colliculi as well as transient expression in neuroepithelium of the cerebral and hippocampal cortices of E16 and E18.5[review] PMID: 22796301
    25. Selective deletion of Tcf7l2 in the pancreas alters glucose homeostasis in mice carrying TCF7L2 risk alleles, indicating the direct role of this factor in controlling beta cell function. PMID: 22717537
    26. A mouse genetics approach shows that removal of TCF4 from beta cells does not affect their function, whereas manipulating TCF4 levels in the liver has major effects on metabolism. In Tcf7l2 double negatice mice, the immediate postnatal surge in liver metabolism does not occur. Consequently, pups die due to hypoglycemia. PMID: 23260145
    27. Data suggest that TCF7L2 and Wnt signaling are negative regulators of gluconeogenesis in hepatocytes; TCF7L2 is among the downstream effectors of insulin in hepatocytes; insulin or feeding stimulates hepatic TCF7L2 expression. PMID: 22967502
    28. these data suggest a crucial role of TCF7L2 in hepatic glucose metabolism; reduced hepatic expression of nuclear isoforms of this factor might be a critical instigator of hyperglycemia in type 2 diabetes PMID: 23028378
    29. During myelin formation, Tcf7l2/beta-catenin is regulated temporally, spatially, and also at levels of expression. PMID: 22160878
    30. The results suggest that decreased expression of Tcf7l2 confers reduction of diabetic susceptibility in mice via regulation on the metabolism of glucose and lipid. PMID: 22719219
    31. Tcf7l2 alters behavior in mice. Importantly, these differences are observed prior to the onset of detectable glucose metabolism abnormalities PMID: 22046400
    32. Tcf7l2 plays a role in regulating glucose tolerance, suggesting that overexpression of this gene is associated with increased risk of T2D. PMID: 21673050
    33. T-cell factor 4 functions as a tumor suppressor whose disruption modulates colon cell proliferation and tumorigenesis. PMID: 21383188
    34. regulates activin receptor-like kinase 1 (ALK1)/Smad1 pathway for development of diabetic nephropathy PMID: 20803090
    35. Wnt/beta-catenin/TCF7L2 pathway is preferentially able to alter the transcriptional regulation of the TGF-beta-target gene, TMEPAI. PMID: 20889500
    36. A minimal promoter for intestinal cell kinase contains functional sites for beta-catenin/TCF7L2 and FOXA. PMID: 20459822
    37. Tcf7l2 is a type 2 diabetes susceptbility gene, and may also regulate obesity. PMID: 19898815
    38. the cell-type-specific complement of TCF4 isoforms is likely to be a major determinant for the context-dependent transcriptional output of Wnt/beta-catenin signalling. PMID: 20044351
    39. Collectively, these patterns of expression suggest that TCF7L2 has distinct functions within the brain, with a general role in the development and maintenance of thalamic and midbrain neurons, and then a distinct role in autonomic homeostasis. PMID: 19845015
    40. Tcf4 and Aes influence pituitary growth and development, and place Tcf4 and Tle3 in the genetic hierarchy with Pro PMID: 12907761
    41. Smad1, beta-catenin and Tcf4 have roles in controlling Myc transcription PMID: 15576399
    42. association of beta-catenin with androgen receptor and T-cell factor 4 (TCF4) in the presence of androgens PMID: 16210377
    43. The results of these studies provide evidence of a mechanistic link between the Wnt/TCF4 and the Msx1/Pax3/MyoD pathways within limb bud muscle precursor cells. PMID: 17727834
    44. Data suggest that TCF4 is necessary both intrinsically and extrinsically to Rathke's pouch to ensure the proper growth of the pituitary gland. PMID: 17919533
    45. Depletion of endogenous progastrin inhibits the tumorigenicity of APC-mutated colorectal cancer cells in vivo by promoting ICAT expression, thereby counteracting beta-catenin/Tcf-4 activity. PMID: 17920061
    46. Reduced TCF7L2 expression reduces glucose-simulated insulin secretion and insulin gene expression while slightly potentiating glucose stimulated changes in intracellular free Ca(2+) concentrations. PMID: 18481957
    47. study mapped developmental expression of Tcf7l2 in developing cerebellum; consensus emerging our data is that expression of Tcf7l2 isoforms comprising or lacking C-clamp motif is tightly correlated with the proliferative status in vivo PMID: 19125404
    48. While TCF7L2 mRNA levels were increased in isolated islets from the diabetic mouse, protein levels were decreased. PMID: 19386626
    49. SDF-1 promotes pancreatic beta cell survival via the stabilisation and activation of beta-catenin/TCF7L2 transcriptional activators. PMID: 19468708
    50. findings show that crosstalk between HDAC1/2 and the canonical Wnt signaling pathway mediated by TCF7L2 serves as a regulatory mechanism for oligodendrocyte differentiation PMID: 19503085

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  • 相关疾病：
    Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC).
  • 亚细胞定位：
    Nucleus, PML body.
  • 蛋白家族：
    TCF/LEF family
  • 组织特异性：
    Detected in adult brain and liver, and at lower levels in intestine, with a clear increase from the distal colon to the duodenum. Detected at low levels in heart, lung, kidney, pituitary and testis.
  • 数据库链接：

    KEGG: mmu:21416

    STRING: 10090.ENSMUSP00000107284

    UniGene: Mm.139815