Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Shugoshin-like 1 (Sgol1)

Recombinant Mouse Shugoshin-like 1 (Sgol1)

  • 中文名称：
    小鼠Sgo1重组蛋白
  • 货号：
    CSB-YP887463MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Sgo1重组蛋白
  • 货号：
    CSB-EP887463MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Sgo1重组蛋白
  • 货号：
    CSB-EP887463MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Sgo1重组蛋白
  • 货号：
    CSB-BP887463MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Sgo1重组蛋白
  • 货号：
    CSB-MP887463MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Sgo1
  • Uniprot No.：
  • 别名：
    Sgo1; Sgol1Shugoshin 1; Shugoshin-like 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-517
  • 氨基酸序列
    MAKERCQKRS FQDTLEDIKN RMKEKRNKNL AGIGKRKSFI VAPGQVPTNT ATLLRYYQDN NRLLVLALEN EKSKVREAQD VILQLRKECY YLTCQLYALK EKLTSRQSEE TTQNWKGRPS DVVSSIDNTT RDLSGKSLQQ IAVEETDCPY QTTEPSPAVT PETQGCDFDS GKVESTDEVL PRTISIRRHL RKDFSNISHS TTLEDCKASP RVAQSLEVKG SRCREVTVTL HRLENVCLWN KDQISLCSRL INPAKITETE VILSSKPEQI ESKHKRARKR RAEQRRTKQR CKSKSSLRSK GNKNKDKQGL PPTTLDGGIG SCDAYDFNLK GTVHPTPFRQ KMNNGCNKET DSSNSEVSDL ECSTSEDESD DLYLPPSKRL RDYRESERAV TRPRSKRGLQ YPDGKERKEV LPSTAPTGIP PETQESPRCS LKDVTNILQC PRVKIRKPSL PPKRREDSPA VALTKRRCST IKSYKEPTLA SKLRRGDPFT DLCFLNSPIF KQKRGMRCPK RRTKQTQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Involved in centromeric enrichment of AUKRB in prometaphase.
  • 基因功能参考文献：
    1. MYCN-promoted SGO1 transcription and SGO1 expression tended to be higher in MYCN- or MYC-overexpressing cancers. Together, these findings indicate that SGO1 plays a role in the DNA damage response in interphase. PMID: 27539729
    2. Study shows Sgo1 expression in heart, gut, eye and other central nervous system tissue during development and post-development. It demonstrates Sgo1 localization in cytoplasmic region in nucleated cells in select organs. PMID: 28465207
    3. Data show that compound mutant spindle assembly checkpoint components BubR1 and Sgo1 embryonic fibroblasts (MEFs) grew at a much slower rate, and a small fraction of cells exhibited morphologies of senescent cells at early passages. PMID: 26847209
    4. Study established the Sgo1 haploinsufficient mouse as a colon cancer model and identified antagonizing oncogenic and tumor-suppressing pathways that are differentially expressed and may be responsible for the different dynamics in the tumor development. PMID: 25773652
    5. Sgo1(-/+)-mediated ME-CIN strongly promoted/progressed development of hepatocellular carcinoma in the presence of an initiator carcinogen, and it had a mild initiator effect by itself. PMID: 25740822
    6. Haploinsufficiency of SGO1 results in genomic instability manifested as missegregation of chromosomes and formation of extra centrosomal foci in both murine embryonic fibroblasts and adult bone marrow cells. PMID: 22262168
    7. It seems that Sgo1 sets up the centromeric protection mechanism in G2, but that its Bub1-dependent localisation to centromeres during mitosis is not required to maintain cohesion. PMID: 20124418
    8. Prevention of premature separation of sister chromatids in meiosis I requires the retention of centromeric Sgo1, while normal separation of sister chromatids in meiosis II requires loss of centromeric Sgo1. PMID: 18949044
    9. report structure and function of the PP2A-shugoshin interaction. PMID: 19716788

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Shugoshin family
  • 组织特异性：
    Ubiquitously expressed in proliferating cells. Moderately expressed in the oocytes.
  • 数据库链接：

    KEGG: mmu:72415

    STRING: 10090.ENSMUSP00000024736

    UniGene: Mm.153202