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  4. Recombinant Mouse Serine/threonine-protein kinase pim-3 (Pim3)

Recombinant Mouse Serine/threonine-protein kinase pim-3 (Pim3)

  • 中文名称：
    小鼠Pim3重组蛋白
  • 货号：
    CSB-YP018014MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pim3重组蛋白
  • 货号：
    CSB-EP018014MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pim3重组蛋白
  • 货号：
    CSB-EP018014MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pim3重组蛋白
  • 货号：
    CSB-BP018014MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pim3重组蛋白
  • 货号：
    CSB-MP018014MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pim3
  • Uniprot No.：
  • 别名：
    Pim3; Serine/threonine-protein kinase pim-3; EC 2.7.11.1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-326
  • 氨基酸序列
    MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKVY QVGAVLGSGG FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGVAVPLE VVLLRKVGAA GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF FAQVLAAVRH CHNCGVVHRD IKDENLLVDL RSGELKLIDF GSGAVLKDTV YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE ILRGRLFFRR RVSPECQQLI EWCLSLRPSE RPSLDQIAAH PWMLGTEGSV PENCDLRLCA LDTDDGASTT SSSESL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis and promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression and protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.
  • 基因功能参考文献：
    1. Loss of PIM3 is associated with abnormal hematopoietic phenotypes. PMID: 23360755
    2. investigation of physiological role of Pim3 in beta-cell function: regulates glucose-stimulated insulin secretion; limits ERK signaling; interacts with SOCS6; Pim3 localized to beta-cell; Pim3 knockout mice display increased glucose tolerance PMID: 21099329
    3. Pim-3 has an important role in modulating c-Myc and PGC-1alpha protein levels and cell growth PMID: 21187426
    4. All three Pim kinase family members predominantly phosphorylate Bad on Ser112 and in addition are capable of phosphorylating Bad on multiple sites associated with the inhibition of the pro-apoptotic function of Bad in HEK-293 cells PMID: 16403219
    5. Pim-3 kinase has a novel role in the control of self-renewal of embryonic stem cells. PMID: 17717068
    6. Pim-3 is expressed in endothelial cells and promotes vascular tube formation. PMID: 19229879

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PIM subfamily
  • 组织特异性：
    Detected in pancreas but exclusively in beta-cells.
  • 数据库链接：

    KEGG: mmu:223775

    STRING: 10090.ENSMUSP00000044603

    UniGene: Mm.400129