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  4. Recombinant Mouse SAM pointed domain-containing Ets transcription factor (Spdef)

Recombinant Mouse SAM pointed domain-containing Ets transcription factor (Spdef)

  • 中文名称：
    小鼠Spdef重组蛋白
  • 货号：
    CSB-YP893875MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Spdef重组蛋白
  • 货号：
    CSB-EP893875MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Spdef重组蛋白
  • 货号：
    CSB-EP893875MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Spdef重组蛋白
  • 货号：
    CSB-BP893875MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Spdef重组蛋白
  • 货号：
    CSB-MP893875MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Spdef
  • Uniprot No.：
  • 别名：
    Spdef; Pdef; Pse; SAM pointed domain-containing Ets transcription factor; Prostate epithelium-specific Ets transcription factor; Prostate-specific Ets; Prostate-derived Ets factor
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-325
  • 氨基酸序列
    MGSASPGLSN VSPGCLLLFP DVAPRTGTEK AASGAMGPEK QEWSPSPPAT PEQGLSAFYL SYFNMYPDDS SWVAKVPEAR AGEDHPEEPE QCPVIDSQAS GSTLDEHSLE QVQSMVVGEV LKDIETACKL LNITADPGDW SPGNVQKWLL WTEHQYRLPP AGKAFQELGG KELCAMSEEQ FRQRAPLGGD VLHAHLDIWK SAAWMKERTS PGTLHYCAST SEEGWTDGEV DSSCSGQPIH LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGVRKNRPAM NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
  • 基因功能参考文献：
    1. In studies of mice and human CRC, we found that SPDEF induces a quiescent state in CRC cells by disrupting binding of b-catenin to TCF1 and TCF3 and regulation of genes that control the cell cycle. PMID: 28390865
    2. SPDEF causes goblet cell differentiation and Th2 inflammation during postnatal development and is required for goblet cell metaplasia and normal Th2 inflammatory responses to house dust mite aeroallergen. PMID: 25866971
    3. SPDEF inhibits prostate carcinogenesis by disrupting a positive feedback loop in regulation of the Foxm1 oncogene. PMID: 25254494
    4. Spdef(-/-) mice have an ocular surface phenotype similar to that in moderate dry eye, providing a new, more convenient model for the disease. PMID: 23665202
    5. SPDEF inhibited the expression of beta-catenin-target genes in mouse colon tumors, and interacted with beta-catenin to block its transcriptional activity in CRC cell lines, resulting in lower levels of cyclin D1 and c-MYC. PMID: 23376423
    6. Our data reveal a novel nonredundant role for Serpinb3a in mediating mucus production through regulation of sterile alpha motif pointed domain containing v-ets avian erythroblastosis virus E26 oncogene homolog transcription factor expression PMID: 21126757
    7. Spdef functions in secretory intestinal cells and suggest a common molecular mechanism for maturation of gastrointestinal secretory lineages. PMID: 20801882
    8. Transcriptional regulation of p21/CIP1 cell cycle inhibitor by PDEF controls cell proliferation and mammary tumor progression PMID: 20139077
    9. Spdef was sufficient to inhibit proliferation of intestinal progenitors and induce differentiation into goblet cells. PMID: 19786015
    10. These data show that SPDEF plays a critical role in regulating a transcriptional network mediating the goblet cell differentiation and mucus hyperproduction associated with chronic pulmonary disorders. PMID: 19759516
    11. SPDEF was induced following intratracheal allergen exposure and after Th2 cytokine stimulation and was sufficient to cause goblet cell differentiation of Clara cells in vivo. PMID: 17347682
    12. The ets-domain transcription factor Spdef promotes maturation of goblet and paneth cells in the intestinal epithelium. PMID: 19549527

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ETS family
  • 组织特异性：
    Expressed in the accessory glands of sex organs including the prostate, seminal vesicle, coagulating gland in males, the oviduct in females, and in intestines. Expression is epithelial-specific.
  • 数据库链接：

    KEGG: mmu:30051

    STRING: 10090.ENSMUSP00000025054

    UniGene: Mm.26768