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  4. Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 2 (Ripk2)

Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 2 (Ripk2)

  • 中文名称：
    小鼠Ripk2重组蛋白
  • 货号：
    CSB-YP019736MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ripk2重组蛋白
  • 货号：
    CSB-EP019736MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ripk2重组蛋白
  • 货号：
    CSB-EP019736MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ripk2重组蛋白
  • 货号：
    CSB-BP019736MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ripk2重组蛋白
  • 货号：
    CSB-MP019736MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ripk2; Receptor-interacting serine/threonine-protein kinase 2; EC 2.7.11.1; Tyrosine-protein kinase RIPK2; EC 2.7.10.2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-539
  • 氨基酸序列
    MNGDAICSAL PPIPYHKLAD LHYLSRGASG TVSSARHADW RVRVAVKHLH IHTPLLDSER NDILREAEIL HKARFSYILP ILGICNEPEF LGIVTEYMPN GSLNELLHRK TEYPDIAWPL RFRILHEIAL GVNYLHNMNP PLLHHDLKTQ NILLDNEFHV KIADFGLSKW RMMSLSQSRS YKSAPEGGTI IYMPPENYEP GQKSRASVKH DIYSYAVIMW EVLSRKQPFE EVTNPLQIMY SVSQGHRPDT SEENLPFDIP HRGLMISLIQ SGWAQNPDER PSFLKCLIEL EPVLRTFEDI TFLEAVIQLK KAKIQSSSST IHLCDKKMDL SLNIPANHPP QEESCGSSLL SRNTGSPGPS RSLSAPQDKG FLSGAPQDCS SLKAHHCPGN HSWDGIVSVP PGAAFCDRRA SSCSLAVISP FLVEKGSERP PIGIAQQWIQ SKREAIVSQM TEACLNQSLD ALLSRDLIMK EDYELISTKP TRTSKVRQLL DTSDIQGEEF AKVVVQKLKD NKQLGLQPYP EVPVLSKAPP SNFPQNKSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Once recruited, autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is release from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling.
  • 基因功能参考文献：
    1. These findings demonstrate that Dectin-1, Irak1, and Rip2 are involved in response to Aspergillus fumigatus infection. Dectin-1 modulates the expression of Irak1 and Rip2. PMID: 29058172
    2. RIP2-knockout attenuates cardiac injury and dysfunction in hypertrophic heart. RIP2-deficience alleviates inflammation in heart of hypertrophic mice. RIP2-deletion inhibits fibrosis in the heart of hypertrophic mice. PMID: 28698147
    3. Overall, our study has identified a new role of PS-341 in the cell death of BMDMs and provided a novel insight into the atherosclerotic inflammation caused by proteasome-mediated macrophage necroptosis. PMID: 27363341
    4. that Rip2 modifies VEGF-induced signalling and vascular permeability in myocardial ischaemia PMID: 26130752
    5. serves as a novel positive modulator of ventricular remodelling after myocardial infarction via the regulation of NF-kappaB and p38 signalling PMID: 26463597
    6. Bacterial polysaccharide-responsive miR-150 and miR-143 RIPK2 and TAK1 to suppress NOD2-induced immunomodulators. PMID: 26391398
    7. NOD2 downregulates colonic inflammation by IRF4-mediated inhibition of K63-linked polyubiquitination of RICK and TRAF6. PMID: 24670424
    8. RIPK2 has a role in NOD signalling and in inflammatory cytokine production PMID: 25778803
    9. Rip2-deficient tumors showed enhanced epithelial-to-mesenchymal transition, with elevated expression of zeb1, zeb2, twist, and snail in the tumor microenvironment. PMID: 24733360
    10. Suggest that the Nod2-Rip2 axis may contribute to the innate immune response of neutrophils against bacterial infection. PMID: 24766550
    11. Nod/Ripk2 signaling in dendritic cells activates IL-17A-secreting innate lymphoid cells and drives colitis in T-bet-/-.Rag2-/- (TRUC) mice. PMID: 24927559
    12. SMS suppressed the activation of NF-kappaB and receptor interacting protein-2/caspase-1. PMID: 23356698
    13. In this work, we show that RIP2 has important role on proinflammatory cytokine production in Y. enterocolitica infected macrophages in TLR4-tolerant condition. PMID: 23952047
    14. we observed for the first time that lung Rip2 protein level was markedly elevated in allergic airway inflammation PMID: 23918989
    15. Our findings identify RIP2 as a substrate for Pellino3 and Pellino3 as an important mediator in the Nod2 pathway and regulator of intestinal inflammation. PMID: 23892723
    16. These results demonstrate a role for NOD2-RIPK2 signaling in protection against virally triggered immunopathology by negatively regulating activation of the NLRP3 inflammasome and production of IL-18 via ULK1-dependent mitophagy. PMID: 23525089
    17. studies demonstrate the crucial role of CARD3 in metabolism and indicate that CARD3 deficiency promotes the diet-induced phenotype of type 2 diabetes. PMID: 23321697
    18. Together, these results revealed that Rip2 plays important roles in the immune control of Anaplasma phagocytophilum and may contribute to our understanding of the host response to Rickettsiales. PMID: 22747758
    19. YopJ subverts the Nod2/RICK/TAK1 pathway and activates caspase-1 to induce intestinal barrier dysfunction. PMID: 22520462
    20. RIPK2/NOD2 signaling plays a pivotal role in the onset of experimental arthritis by triggering an interleukin (IL)-17-dependent immune response in the knee joint. PMID: 22491249
    21. SNP association with tick burden was confirmed and quantitative and qualitative differences in antibody production were observed between RIPK2 -/- and wild-type mice. PMID: 22314416
    22. Alcohol-fermented soybean increases the production of NO and TNF-alpha through the activation of Rip2/IKK-beta in rIFN-gamma-primed macrophages. PMID: 21895414
    23. Mice transplanted with Rip2(-/-) bone marrow increased macrophage lipid uptake & atherosclerotic lesions despite impaired inflammation. Rip2 is a key regulator of cellular lipid metabolism & cardiovascular disease. PMID: 21959219
    24. RIP2 contributes to neutrophil-dependent host defense against an extracellular Gram-negative pathogen via (i) IL-17A regulation and (ii) neutrophil mobilization to the blood. PMID: 21844230
    25. promotes inflammation and autoimmunity in central nervous system-infiltrating dendritic cells PMID: 21236705
    26. results highlight the importance of Rip2 for Nod-dependent induction of innate and adaptive immunity PMID: 21469090
    27. Mice deleted for the tumour suppressor LKB1 (also called STK11) in the spinal cord, some parts of the brain and in the endocrine pancreas (betaLKB1KO mice) develop hind-limb dysfunction and axon degeneration at about 7 weeks. PMID: 21135058
    28. RIP2 undergoes autophosphorylation on Tyr 474 and this event is necessary for effective NOD2 signaling PMID: 21123652
    29. knock-out mice display immature dendritic cell phenotype PMID: 19853061
    30. Rip2 has an important role in TCR-induced NF-kappaB activation and T-cell function PMID: 14638696
    31. Rip2/Cardiak/Rick is a stress-inducible upstream modulator of pro-caspase-1 apoptotic activation. PMID: 14663141
    32. Regulation of RIPK 2 expression is tissue-specifically associated with M. leprae infection. PMID: 15203566
    33. Rip-like interacting caspase-like apoptosis-regulatory protein kinase (RICK) is a key component of a pathway leading to NF-kappaB induction in inflammatory bowel disease PMID: 15289440
    34. functions as an adaptor molecule, independent of its kinase activity, in transducing signals from immune receptors PMID: 15691841
    35. RICK/nuclear factor-kappaB and p38 signaling have roles in the inflammatory response in a murine model of Crohn disease PMID: 15691843
    36. CARD6 is a regulator of NF-kappaB activation that modulates the functions of RICK protein PMID: 16418290
    37. The kinase RICK is required for stimulation of primary peritoneal mesothelial cells and the induction of potent secretion of chemokines CXCL1 and CCL2 as well as expression of inducible nitric oxide synthase. PMID: 17579072
    38. data linking Rip2 to NOD2 and not Toll-like receptor signaling, M. tuberculosis-induced Rip2 polyubiquitination appears MyD88-independent. PMID: 17947236
    39. These data indicate that differential expression of rip genes can be associated with abnormal growth and differentiation behaviour of skeletal myoblasts. PMID: 17961803
    40. a critical role for RIP2 in Nod signaling, while T cell proliferation, T helper differentiation and TLR responses were unaffected by the absence of RIP2. PMID: 18085666
    41. The RIP2-p38 MAPK signaling axis was examined in the heart. PMID: 18310079
    42. Absence of RIP2 does not affect TCR signalling or T-cell immune response. PMID: 18403232
    43. RIP2 deficiency was associated with defective NOD signaling, but this did not affect T-cell receptor (TCR)-dependent activation of the canonical NF-kappaB signaling or expression of NF-kappaB genes in rejecting allografts PMID: 18522545
    44. upregulation of RIP2 expression is required for rapid resolution of peritonitis PMID: 18566542
    45. These results demonstrate that RICK contributes to the induction of proinflammatory responses and susceptibility to gram-negative bacteria after exposure to LPS. PMID: 19188356
    46. Pharmacological postconditioning effect of muramyl dipeptide is mediated through RIP2 and TAK1. PMID: 19213760

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: mmu:192656

    STRING: 10090.ENSMUSP00000038833

    UniGene: Mm.112765