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  4. Recombinant Mouse Proprotein convertase subtilisin/kexin type 4 (Pcsk4)

Recombinant Mouse Proprotein convertase subtilisin/kexin type 4 (Pcsk4)

  • 中文名称：
    小鼠Pcsk4重组蛋白
  • 货号：
    CSB-YP017643MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pcsk4重组蛋白
  • 货号：
    CSB-EP017643MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pcsk4重组蛋白
  • 货号：
    CSB-EP017643MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pcsk4重组蛋白
  • 货号：
    CSB-BP017643MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pcsk4重组蛋白
  • 货号：
    CSB-MP017643MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pcsk4
  • Uniprot No.：
  • 别名：
    Pcsk4; Nec-3; Nec3; Proprotein convertase subtilisin/kexin type 4; EC 3.4.21.-; KEX2-like endoprotease 3; Neuroendocrine convertase 3; NEC 3; Prohormone convertase 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    111-655
  • 氨基酸序列
    SLVVPTDPWF SKQWYMNKEI QQDLNILKAW NQGLTGRGVV ISILDDGIEK DHPDLWANYD PLASYDFNDY DPDPQPRYTP NDENRHGTRC AGEVSATANN GFCGAGVAFN ARIGGVRMLD GAITDIVEAQ SLSLQPQHIH IYSASWGPED DGRTVDGPGL LTQEAFRRGV TKGRQGLGTL FIWASGNGGL HYDNCNCDGY TNSIHTLSVG STTRQGRVPW YSEACASTFT TTFSSGVVTD PQIVTTDLHH QCTDKHTGTS ASAPLAAGMI ALALEANPLL TWRDLQHLVV RASRPAQLQA EDWRINGVGR QVSHHYGYGL LDAGLLVDLA RVWLPTKPQK KCAIRVVHTP TPILPRMLVP KNVTACSDGS RRRLIRSLEH VQVQLSLSYS RRGDLEIFLT SPMGTRSTLV AIRPLDISGQ GYNNWIFMST HYWDEDPQGL WTLGLENKGY YFNTGTLYYY TLLLYGTAED MTARPQAPQV TSRARACVQR DTEGLCQESH SPLSILAGLC LISSQQWWWL YSHPQQPVTE GQASCHPPVT PAAAA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proprotein convertase involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. In males, important for ADAM2 processing as well as other acrosomal proteins with roles in fertilization and critical for normal fertilization events such as sperm capacitation, acrosome reaction and binding of sperm to zona pellucida. Plays also a role in female fertility, involved in the regulation of trophoblast migration and placental development, may be through the proteolytical processing and activation of proteins such as IGF2. May also participate in folliculogenesis in the ovaries.
  • 基因功能参考文献：
    1. CRES may be a regulator of PC4 in sperm-egg interaction and fertilization. PMID: 22827436
    2. Data suggest that the fertility defect in PCSK4 knockout mice is due, in part, to altered processing/proteolysis of ACRBP (proacrosin/acrosin binding protein sp32) as well as structural abnormalities in sperm heads and acrosomes. PMID: 22357636
    3. thatADAM2may be a natural substrate of sperm PCSK4 and its cleavage by the enzyme during acrosome reaction may be relevant to the fertilization process. PMID: 21302280
    4. Efficient activation and trafficking of PCSK4 requires a special environment, which presumably exists in testicular germ cells where it is highly expressed, but not in somatic cells where it is not or is barely expressed. PMID: 21080038
    5. Alterations in signal transduction and proteolytic processing during in vitro capacitation may underlie the fertilization incompetence of PCSK4-null sperm. PMID: 19342015
    6. PCSK4-null sperm underwent capacitation at a faster rate; they were induced to acrosome react by lower concentrations of zona pellucida; and their egg-binding ability was only half that of wild-type sperm. PMID: 16371590

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, acrosome membrane.
  • 蛋白家族：
    Peptidase S8 family, Furin subfamily
  • 组织特异性：
    Expressed abundantly in the testis since postnatal Day 16. In testis, strongly detected in round and elongated spermatids as well as spermatocytes. Also observed in residual bodies engulfed by Sertoli cells at spermatogenic stages VIII and IX. In ovaries,
  • 数据库链接：

    KEGG: mmu:18551

    STRING: 10090.ENSMUSP00000020340

    UniGene: Mm.299916