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  4. Recombinant Mouse Oocyte-expressed protein homolog (Ooep)

Recombinant Mouse Oocyte-expressed protein homolog (Ooep)

  • 中文名称：
    小鼠Ooep重组蛋白
  • 货号：
    CSB-YP883924MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ooep重组蛋白
  • 货号：
    CSB-EP883924MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ooep重组蛋白
  • 货号：
    CSB-EP883924MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ooep重组蛋白
  • 货号：
    CSB-BP883924MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ooep重组蛋白
  • 货号：
    CSB-MP883924MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ooep
  • Uniprot No.：
  • 别名：
    Ooep; Oep19; Sddr; Oocyte-expressed protein homolog; Factor located in oocytes permitting embryonic development; Floped; Oocyte- and embryo-specific protein 19; mOEP19; STAT3 downstream gene and differentiation regulator
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-164
  • 氨基酸序列
    MASHTADADA KPDSDSQKLL NVLPVSLRLR TRPWWFPIQE VSNPLVLYME AWVAERVIGT DQAEISEIEW MCQALLTVDS VNSGNLAEIT IFGQPSAQTR MKNILLNMAA WHKENELQRA VKVKEVEEFL KIRASSILSK LSKKGLKLAG FPLPLEGRET QMES
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart. Positively regulates the homologous recombination-mediated DNA double-strand break (DSB) repair pathway by regulating ATM activation and RAD51 recruitment to DSBs in oocytes. Thereby contributes to oocyte survival and the resumption and completion of meiosis. As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning.
  • 基因功能参考文献：
    1. role in homologous recombination-mediated DNA double-strand break repair in oocytes PMID: 29955025
    2. FLOPED is part of the subcortical maternal complex (SCMC). SCMC is required for formation of the cytoplasmic F-actin meshwork that controls the central position of the spindle and ensures symmetric division. PMID: 25208553
    3. Floped-/- blastocysts had significantly decreased derivation rates. PMID: 23776005
    4. The CES5/OOEP is an essential component of the oocyte cytoplasmic lattices and is required for embryonic development at the maternal-zygotic stage transition. PMID: 20590823
    5. MOEP19 domain directs RNAs essential for normal embryonic development to specific locations in the oocyte and early embryo. PMID: 18191828
    6. Results describe a subcortical maternal complex, containing Mater, Floped, TLE6, and Filia, that is essential for preimplantation mouse embryogenesis. PMID: 18804437

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    KHDC1 family
  • 组织特异性：
    Expressed in ovaries, where it is restricted to growing oocytes, with greatest levels in fully grown oocytes.
  • 数据库链接：

    KEGG: mmu:67968

    STRING: 10090.ENSMUSP00000034900

    UniGene: Mm.26883