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  4. Recombinant Mouse Nucleolysin TIA-1 (Tia1)

Recombinant Mouse Nucleolysin TIA-1 (Tia1)

  • 中文名称：
    小鼠Tia1重组蛋白
  • 货号：
    CSB-YP023527MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tia1重组蛋白
  • 货号：
    CSB-EP023527MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tia1重组蛋白
  • 货号：
    CSB-EP023527MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tia1重组蛋白
  • 货号：
    CSB-BP023527MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tia1重组蛋白
  • 货号：
    CSB-MP023527MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tia1
  • Uniprot No.：
  • 别名：
    Tia1; Tia; Nucleolysin TIA-1; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-386
  • 氨基酸序列
    MEDEMPKTLY VGNLSRDVTE ALILQLFSQI GPCKNCKMIM DTAGNDPYCF VEFHEHRHAA AALAAMNGRK IMGKEVKVNW ATTPSSQKKD TSSSTVVSTQ RSQDHFHVFV GDLSPEITTE DIKAAFAPFG RISDARVVKD MATGKSKGYG FVSFFNKWDA ENAIQQMGGQ WLGGRQIRTN WATRKPPAPK STYESNTKQL SYDEVVSQSS PNNCTVYCGG VTSGLTEQLM RQTFSPFGQI MEIRVFPDKG YSFVRFSSHE SAAHAIVSVN GTTIEGHVVK CYWGKETLDM INPVQQQNQI GYPPTYGQWG QWYGNAQQIG QYVPNGWQVP AYGVYGQPWS QQGFNQTQSS APWMGPNYSV PPPQGQNGSM LPSQPAGYRV AGYETQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
  • 基因功能参考文献：
    1. Results demonstrate that neither the basal nor seizure-induced expression profiles of COX-2 were altered in mice lacking a functional TIA-1 gene suggesting that TIA-1 does not contribute to regulation of COX-2 protein expression in neurons. Induced seizure threshold was also unchanged in mice lacking TIA-1 protein, indicating that this RNA binding protein does not influence the innate seizure threshold. PMID: 29337236
    2. Tia1 controls p53 mRNA translation in B cells. PMID: 28904350
    3. TIA1 knockdown or knockout inhibits tau misfolding and associated toxicity in cultured hippocampal neurons, while overexpressing TIA1 induces tau misfolding and stimulates neurodegeneration. PMID: 27160897
    4. This study showed that reactive oxygen species such as H2O2 oxidize the cytoplasmic stress granules (SG)-nucleating protein TIA1, thereby inhibiting SG assembly. PMID: 26738979
    5. Genetic ablation of the stress granule nucleator TIA-1 has a novel major effect on mRNAs encoding lipid homeostasis factors in the brain, similar to the fasting effect. PMID: 24659297
    6. TIA proteins can function as long-term regulators of the ACTB mRNA metabolism in mouse and human cells. PMID: 24766723
    7. TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation. PMID: 24696465
    8. Either TIA1 or TIAR inactivation broadly alter normal development-associated signalling pathways in murine embryonic fibroblasts. PMID: 24086455
    9. TIA-1 as a negative regulator of allergen-mediated pulmonary inflammation in vivo. PMID: 22525013
    10. Data suggest that TIA-1 functions as a translational silencer of cyclooxygenase-2 (COX-2) expression and support the hypothesis that dysregulated RNA-binding of TIA-1 promotes COX-2 expression in neoplasia. PMID: 12885872
    11. TIA-1 and TTP are genetic modifiers of inflammatory arthritis that can alter the spectrum of cells that produce arthritogenic cytokines PMID: 14769925
    12. TIAR regulates the relative expression of TIA-1 isoforms. PMID: 17488725
    13. TIA-1-induced polysome disassembly is required for enhanced mRNA decay PMID: 17711853
    14. The TIA-1 beta-loop is involved in and is necessary and sufficient for RNA-binding activity of the TIA-1 second RNA recognition motif (RRM2), together with the beta-sheet surface. PMID: 18500819
    15. TIA1 and TIAL1 regulate the alternate splicing of lysyl hydroxylase 2 PMID: 19110540
    16. The intronic splicing enhancer of minute virus of mice binds the cellular RNA-processing proteins TIA-1 and TIAR, which enhance usage of the nonconsensus donor. PMID: 19339348

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  • 亚细胞定位：
    Cytoplasm, Stress granule. Nucleus.
  • 数据库链接：

    KEGG: mmu:21841

    STRING: 10090.ENSMUSP00000093425

    UniGene: Mm.333219