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  4. Recombinant Mouse Lymphocyte antigen 96 (Ly96)

Recombinant Mouse Lymphocyte antigen 96 (Ly96)

  • 中文名称：
    小鼠Ly96重组蛋白
  • 货号：
    CSB-EP013254MO
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ly96; Esop1; Md2; Lymphocyte antigen 96; Ly-96; ESOP-1; Protein MD-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    32.4kDa
  • 表达区域：
    19-160aa
  • 氨基酸序列
    EKQQWFCNSSDAIISYSYCDHLKFPISISSEPCIRLRGTNGFVHVEFIPRGNLKYLYFNLFISVNSIELPKRKEVLCHGHDDDYSFCRALKGETVNTSIPFSFEGILFPKGHYRCVAEAIAGDTEEKLFCLNFTIIHRRDVN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds bacterial lipopolysaccharide (LPS). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS.
  • 基因功能参考文献：
    1. We conclude that MD2 is a significant contributor in the Ang II-induced kidney inflammatory injury in chronic renal diseases. PMID: 28322341
    2. Blockade of MD2 prevents obesity-induced inflammation and nephropathy. PMID: 28767204
    3. MD2 is essential to obesity-related cardiac hypertrophy through activating JNK/ERK and NF-kappaB-dependent cardiac inflammatory pathways. Targeting MD2 would be a therapeutic approach to prevent obesity-induced cardiac injury and remodeling. PMID: 28965884
    4. RTFs contribute to the regulation of LPS-induced inflammatory response in RAW264.7 cells through TLR4/MD-2 mediated NF-kappaB and JNK pathway. It PMID: 27235587
    5. This study provides evidence that MD2 plays a key role in the pathogenesis of retinal I/R damage. PMID: 29111459
    6. Data suggest that C4bp prevents interaction between Tlr4/MD-2 and its ligand; C4bp does not appear to interact with Tlr3; C4bp binds to macrophage surface Tlr4 and inhibits Tlr/Tlr ligand interaction, thereby inhibiting Tlr4 activation. (C4bp = complement component 4 binding protein; Tlr = toll-like receptor; MD-2 = myeloid differentiation protein-2) PMID: 28542817
    7. MD2 plays an important role in induction of allergic sensitization to cat dander and common pollens relevant to human allergic diseases. PMID: 26586036
    8. Oxidative stress in retinal ischemia-reperfusion injury activates TLR4 signaling via MD2. PMID: 28063877
    9. Neoseptin-3 and lipid A form dissimilar molecular contacts to achieve receptor activation; hence strong TLR4/MD-2 agonists need not mimic LPS PMID: 26831104
    10. Here we demonstrate that cholesterol binds to myeloid differentiation-2 (MD-2), a TLR4 ancillary molecule. PMID: 26806306
    11. MD-2 is a critical regulator of the establishment of allergic airway sensitization to HDM in mice. Serum MD-2 may represent a potential biomarker for the amplification of allergic sensitization and allergic inflammation. PMID: 26344079
    12. Data show that myeloid differentiation factor 2 (MD-2) binds specifically to disulfide isoform of box protein 1, high mobility group (HMGB1) to facilitate toll-like receptor 4 (TLR4)-dependent signaling. PMID: 25559892
    13. Carbon monoxide treatment reduces the expression of the TLR4/MD2 complex on the surface of myeloid cells, which renders them resistant to lipopolysaccharide priming in vitro, as well as in vivo in a model of endotoxic shock. PMID: 25179131
    14. Mechanistically, engagement of MD-2 by PTX3-opsonized Aspergillus conidia activated the TLR4/Toll/IL-1R domain-containing adapter inducing IFN-beta-dependent signaling pathway converging on IL-10. PMID: 25049357
    15. SAA3 directly binds MD-2 and activates the MyD88-dependent TLR4/MD-2 pathway. PMID: 23858030
    16. Monophosphoryl lipid A is unable to efficiently form TLR4/MD-2 heterotetramers, but it still needs heterotetramer formation for the full extent of signaling it is able to achieve. PMID: 23638128
    17. Data show that rifampin binds to myeloid differentiation protein 2 (MD-2), the key coreceptor for innate immune TLR4. PMID: 23568774
    18. Gb4 is an endogenous ligand for TLR4-MD-2 and is capable of attenuating LPS toxicity, indicating the possibility for its therapeutic application in endotoxin shock. PMID: 23471986
    19. Data provide structural evidence of the agonistic property of lipid IVa on TLR4/MD-2 and deepen understanding of the ligand binding and dimerization mechanism by the structurally diverse Lipopolysaccharide (LPS) variants. PMID: 22532668
    20. GL and ILG modulate the TLR4/MD-2 complex at the receptor level, leading to suppress LPS-induced activation of signaling cascades and cytokine production PMID: 22422925
    21. Data show that morphine binds to an accessory protein of Toll-like receptor 4 (TLR4), myeloid differentiation protein 2 (MD-2), thereby inducing TLR4 oligomerization and triggering proinflammation. PMID: 22474354
    22. Data show that LPS priming of tolerant FLDCs inhibited the up-regulation of TLR4/MD-2 expression. PMID: 21802073
    23. According to our murine TLR4/MD-2-activation model, the two phosphates on lipid A were predicted to interact extensively with the two positively charged patches on mouse TLR4activation PMID: 21865549
    24. Demonstrate a novel, critical role for MD-2 and TLR4 through NADPH activation in liver steatosis, and fibrosis in a NASH model in mice. PMID: 21233280
    25. species-specific activation of lipid IV(A) PMID: 20592019
    26. Neutralizing toll-like receptor 4/myeloid differentiation protein-2 is highly efficacious in protecting against bacterial infection-induced toxemia. PMID: 17947685
    27. MD-2-mediated ionic interactions between lipid A and TLR4 are essential for receptor activation PMID: 20018893
    28. MD-2 in complex with toll-like receptor 4 mediates signal transduction induced by the amino acid-containing bacterial lipid, flavolipin. PMID: 11884465
    29. MD-2 is essential for correct intracellular distribution and LPS-recognition of TLR4. PMID: 12055629
    30. By alanine-scanning mutagenesis of MD-2, important amino acid residues have been identified that confer lipopolysaccharide and taxol responsiveness on TLR4 and enable formation of cell surface TLR4-MD-2 complex recognized by specific monoclonal antibody. PMID: 12496426
    31. TLR4 and its partner molecule MD-2 may play an important role in Kupffer cell activation and ischemia-reperfusion injury. PMID: 15334694
    32. Results show that the N-terminal region of toll-like receptor 4 is essential for association with MD-2, which is required for the cell surface expression and hence the responsiveness to lipopolysaccharide. PMID: 15337750
    33. Collectively, MD-2 is essential for the recognition of LPS by TLR4 but not for that of PGN by TLR2 of mast cells. PMID: 15369778
    34. Results indicate that amino acid residues 57, 61, and 122 of mouse MD-2 are critical to determine the agonist-antagonist activity of lipid IVa and suggest that these amino acid residues may be involved in the discrimination of lipid A structure. PMID: 16407172
    35. agonistic mAb to Toll-Like Receptor 4 (TLR4)/MD-2 protected mice from lipopolysaccharide/d-galactosamine-induced acute lethal hepatitis by delivering a protective signal activating NF-kappaB through TLR4/MD-2 PMID: 16547261
    36. This study shows regulatory roles for MD-2 in initiating and terminating ligand-induced TLR4 oligomerization. PMID: 16670331
    37. a unique complex of TLR4, MD-2, and CD44 recognizes hyaluronan in signaling tissue injury PMID: 17400552
    38. Data show that lipopolysaccharides act through both MyD88-dependent and -independent TLR4/MD2 signaling pathways to directly inhibit GHR gene expression. PMID: 17601656
    39. Based on structural analysis and mutagenesis experiments on MD-2 and TLR4, we propose a model of TLR4-MD-2 dimerization induced by LPS. PMID: 17803912
    40. The lower expression of the CD14 and TLR-4/MD-2 receptors may be partly responsible for the immunodeficiency observed in the malnourished mice. PMID: 17950615
    41. MD-2 is a newly recognized type II acute-phase reactant, an opsonin for Gram-negative bacteria, and a cofactor essential for the activation of TLR4-expressing cells. PMID: 18056837
    42. role of the TLR4/MD-2 signaling axis in bacterial recognition by phagocytes PMID: 18203953
    43. Data compare the inflammatory potency of two types of Neisseria meningitidis endotoxins in lungs: wild type (hexaacylated, LOS(wt)) and mutant type (pentaacylated, LOS(msbB)), and describe the importance of MD-2 in endotoxin responses in lungs in vivo. PMID: 18203970
    44. The species-specific difference between human and murine MD-2 activation of TLR4 by PTX can be explained by alterations of surface charge distribution (i.e. electrostatic potential), binding pocket size, and the locus of PTX binding within the MD-2 pocket PMID: 18650420
    45. Myeloid differentiation protein-2 has an important role in the CD14-independent LPS-mediated cascade of neutrophil influx PMID: 18988922
    46. the leucine at position 815 is required for the normal maturation of TLR4 and for formation of the TLR4.MD-2 complex. PMID: 19064998
    47. These findings reveal novel roles of lysines 122, 125, and 58 in human MD-2 that contribute to the functional differences between human and murine MD-2 and, potentially, to differences in the sensitivity of humans and mice to endotoxin. PMID: 19783674

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  • 亚细胞定位：
    Secreted, extracellular space. Secreted.
  • 组织特异性：
    Highly expressed in spleen, bone marrow, thymus, liver, kidney, ovary and decidua. Detected at lower levels in testis, small intestine and skin.
  • 数据库链接：

    KEGG: mmu:17087

    STRING: 10090.ENSMUSP00000026881

    UniGene: Mm.116844