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  4. Recombinant Mouse Glutamate decarboxylase 1 (Gad1)

Recombinant Mouse Glutamate decarboxylase 1 (Gad1)

  • 中文名称：
    小鼠Gad1重组蛋白
  • 货号：
    CSB-YP009159MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Gad1重组蛋白
  • 货号：
    CSB-EP009159MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Gad1重组蛋白
  • 货号：
    CSB-EP009159MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Gad1重组蛋白
  • 货号：
    CSB-BP009159MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Gad1重组蛋白
  • 货号：
    CSB-MP009159MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gad1
  • Uniprot No.：
  • 别名：
    Gad1; Gad67Glutamate decarboxylase 1; EC 4.1.1.15; 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-593
  • 氨基酸序列
    MASSTPSPAT SSNAGADPNT TNLRPTTYDT WCGVAHGCTR KLGLKICGFL QRTNSLEEKS RLVSAFRERQ SSKNLLSCEN SDQGARFRRT ETDFSNLFAQ DLLPAKNGEE QTAQFLLEVV DILLNYVRKT FDRSTKVLDF HHPHQLLEGM EGFNLELSDH PESLEQILVD CRDTLKYGVR TGHPRFFNQL STGLDIIGLA GEWLTSTANT NMFTYEIAPV FVLMEQITLK KMREIVGWSN KDGDGIFSPG GAISNMYSIM AARYKYFPEV KTKGMAAVPK LVLFTSEHSH YSIKKAGAAL GFGTDNVILI KCNERGKIIP ADLEAKILDA KQKGYVPLYV NATAGTTVYG AFDPIQEIAD ICEKYNLWLH VDAAWGGGLL MSRKHRHKLS GIERANSVTW NPHKMMGVLL QCSAILVKEK GILQGCNQMC AGYLFQPDKQ YDVSYDTGDK AIQCGRHVDI FKFWLMWKAK GTVGFENQIN KCLELADYLY AKIKNREEFE MVFDGEPEHT NVCFWYIPQS LRGVPDSPER REKLHRVAPK IKALMMESGT TMVGYQPQGD KANFFRMVIS NPAATQSDID FLIEEIERLG QDL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the production of GABA.
  • 基因功能参考文献：
    1. Our results demonstrate that GAD67 plays an important role in Alzheimer's disease pathology PMID: 29017573
    2. Results suggest age-dependent decrease of GAD65/67 mRNAs but normal densities of certain GABAergic interneurons in the Shank3 transgenic mice. PMID: 28400125
    3. These findings reveal that Th and Gad1 share a transcription regulatory mechanism that facilitates odorant-dependent regulation of dopamine and GABA expression levels. PMID: 28411275
    4. This study demonstrated that Alterations in hypoglossal motor neurons due to GAD67 deficiency in mice. PMID: 27956032
    5. Treadmill running prevented partial sciatic nerve ligation-induced reductions in GAD65/67 production, and, thus, GABA levels may be retained in interneurons and neuropils in the superficial dorsal horn. PMID: 27030712
    6. Gad1 knockdown mice have pronounced sensorimotor gating deficits, increased novelty-seeking and reduced fear extinction PMID: 25623945
    7. Glutamate decarboxylase expression may be a reliable proxy of altered GABAergic transmission. PMID: 26370162
    8. Activity deprivation due to TTX preferentially down-regulated GAD67. BDNF-induced increase in the GAD67 protein was markedly lower than that of GAD65, indicating that BDNF differentially regulates activity-dependent gene expression of the 2 isoforms. PMID: 26241953
    9. The results of this study indicated that the mice heterozygous for GAD67 deficiency primarily in PV neurons share several neurochemical and behavioral abnormalities with schizophrenia. PMID: 25904362
    10. Data demonstrate that GAD67 levels directly influence synaptic inhibition. PMID: 24275833
    11. The GAD65-independent mechanism for targeting of GAD67 to synaptic vesicles in neurons is not functional in islet beta-cells. PMID: 25647668
    12. Results indicate the significant contribution of not only GAD65, GAD67 and VGAT-mediated GABAergic but also glycinergic transmissions to both palate and abdominal wall formations PMID: 25545713
    13. These findings provide original evidence that striatal Gad67 expression is involved in the modulation of learning and social behavior. PMID: 24952328
    14. Analysis of GAD65/67 single and double knock-out embryos revealed that the two GADs play complementary (inhibitory) roles in GnRH migration ultimately modulating the speed and/or direction of GnRH migration. PMID: 25125027
    15. Study reports the identification and characterization of novel GAD1 splicing isoforms, showing GAD1 gene in mouse brain is extensively regulated at the level of transcription PMID: 25322942
    16. Gad67-GFP mice results in a sex-dependent deregulation of gamma-aminobutyric acid levels and GABA transporter expression. PMID: 24991763
    17. study found that re-expression of GAD67 in neurons following stroke may play a role in aberrant cell cycle activation, consequently being pro-apoptotic PMID: 24983209
    18. Prenatal stress, in addition to heterozygous deletion of Gad1, could specifically disturb the proliferation of neurons destined to be PV-positive GABAergic interneurons. PMID: 24618690
    19. GAD67+/- mice show reduced sociability and decreased intermale aggression, but normal nest building and urine marking behavior, as well as unchanged locomotor activity and anxiety-like behavior. PMID: 24612522
    20. The adoption of the GAD67-GFP mouse model will certainly boost future functional imaging and gene expression analysis of the mouse NEB microenvironment. PMID: 23568330
    21. Sex differences in reproductive genes Gad1, Kiss1 and Gnrh1 are critically disturbed in GABAB1-deficient mice, probably altering the organization and development of neural circuits governing the reproductive axis. PMID: 24080944
    22. In the absence of GAD67, the electrical activity underlying glutamatergic waves shows increased tendency to burst, indicating that GABAergic inputs inhibit the glutamatergic pacemaker, even when GABAergic pacemaker circuitry can itself initiate waves. PMID: 24623764
    23. Orientation selectivity is decreased in mice in which GAD67 and therefore GABA are down-regulated during development. PMID: 23533083
    24. GAD67 expression in dentate gyrus granule cells is a transient marker of late cell differentiation that persists throughout life. PMID: 23565079
    25. Prenatally immune-challenged offspring exhibit reduced glutamic acid decarboxylase 65-kDa (GAD65) and 67-kDa (GAD67) gene expression in the adult medial prefrontal cortex and dorsal hippocampus. PMID: 23876745
    26. We failed to provide proof-of-concept evidence that lower PV and GAT1 expression in schizophrenia are a consequence of lower GAD67 expression PMID: 23103418
    27. Behavioral experiments showed that LV knockdown of GAD67 results in a deficit in the extinction, but not the acquisition or retention, of fear in mice. Diminished GAD67 in the amygdala blunted the anxiolytic-like effect of diazepam PMID: 23149445
    28. This study demonistrated that both protein and mRNA expression of GAD67 in the whole septum was up-regulated in postpartum mice. PMID: 22750123
    29. The mouse lateral striatum concentrates medium-sized projection neurons with high-level expression of GAD1, but not of GAD2, mRNA. PMID: 22332935
    30. activity-driven expression of GAD67 critically controls GABA synthesis and, thus, vesicular filling of the transmitter PMID: 22723692
    31. Anti-idiotypic antibodies specific to GAD65Ab have a protective role in type 1 diabetes PMID: 22384267
    32. reduced GAD67 mRNA expression can induce lower CB1R mRNA expression. PMID: 22036037
    33. At the mRNA level, the expression of GAD67 and of St8SiaII was significantly reduced by chronic stress in the amygdala of adult mice. PMID: 21819983
    34. a GAD67 heterozygous deletion itself enhances vulnerability by many aspects, e.g., maternal stress, maternity, and being in utero. PMID: 21185888
    35. Cleft palate is caused by CNS dysfunction in Gad1 and Viaat knockout mice PMID: 20333300
    36. Down-regulation of GAD67 is associated with autoimmune type 1 diabetes mellitus. PMID: 20414703
    37. Localized expression of Gad1 transcripts in multiple differentiated non-neural tissues of mouse embryos suggests a wider role for GABA signaling in development than was previously appreciated. PMID: 11178105
    38. glucose responsiveness and transcription mediated by PDX-1 PMID: 12150938
    39. No significant GAD67 gene expression differences are detected in either drug-naive or acute ethanol withdrawn mouse brain. PMID: 12691782
    40. GAD67 expression decreases in frontal cortex, parietal cortex, globus pallidus, entopeduncular nucleus and substantia nigra pars reticulata of R6/2 transgenic mice; at 12.5 weeks of age the pathology differs from that in humans with Huntington's disease PMID: 12950446
    41. RELN and GAD67 promoters express an increased recruitment of methyl-CpG binding domain proteins. PMID: 16113080
    42. GAD67 is localized in the dermis and is potentially involved in variety of skin activities. PMID: 17113713
    43. histone hyperacetylation induces demethylation of reelin and 67-kDa glutamic acid decarboxylase promoters PMID: 17360583
    44. lack of gad67 impairs the function of the respiratory network in mouse fetuses and the impairment progresses with fetal age PMID: 17418495
    45. Temporal migration of gonadotrophin-releasing hormone-1 neurones is modified in GAD67 knockout mice. PMID: 18081557
    46. Mice expressing green fluorescent protein (GFP)under the control of GAD67 promoter, exhibit numerous GFP-positive neurons in the reticular formation during rapid eye movement sleep. PMID: 18215233
    47. In GAD67 deficient mouse, there was reduced level of GABA (16-44%)and inhibitory synaptic transmission to Purkinje cells was seriously impaired. PMID: 18384748
    48. the GABA shunt of SUR1(-/-) islets is blocked by about 75% and showed that this defect is due to decreased glutamate decarboxylase synthesis PMID: 18445600
    49. Glutamate-induced excitotoxic stimulation of NMDAR in cultured cortical neurons leads to altered GAD protein levels from GABAergic neurons through intracellular calcium increase and protease activation. PMID: 18599042
    50. Deletion of GAD67 in Dopamine Receptor-1 Expressing Cells Causes Specific Motor Deficits. PMID: 18615733

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  • 蛋白家族：
    Group II decarboxylase family
  • 数据库链接：

    KEGG: mmu:14415

    STRING: 10090.ENSMUSP00000092539

    UniGene: Mm.272120