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  4. Recombinant Mouse E3 ubiquitin-protein ligase SIAH1A (Siah1a)

Recombinant Mouse E3 ubiquitin-protein ligase SIAH1A (Siah1a)

  • 货号：
    CSB-YP352178MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP352178MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP352178MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP352178MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP352178MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Siah1a
  • Uniprot No.：
  • 别名：
    Siah1a; E3 ubiquitin-protein ligase SIAH1A; EC 2.3.2.27; RING-type E3 ubiquitin transferase SIAH1A; Seven in absentia homolog 1a; Siah-1a; Siah1a; mSiah-1a
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-282
  • 氨基酸序列
    MSRQTATALP TGTSKCPPSQ RVPALTGTTA SNNDLASLFE CPVCFDYVLP PILQCQSGHL VCSNCRPKLT CCPTCRGPLG SIRNLAMEKV ANSVLFPCKY ASSGCEITLP HTEKAEHEEL CEFRPYSCPC PGASCKWQGS LDAVMPHLMH QHKSITTLQG EDIVFLATDI NLPGAVDWVM MQSCFGFHFM LVLEKQEKYD GHQQFFAIVQ LIGTRKQAEN FAYRLELNGH RRRLTWEATP RSIHEGIATA IMNSDCLVFD TSIAQLFAEN GNLGINVTIS MC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Required for completion of meiosis I in males. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4.
  • 基因功能参考文献：
    1. Depletion of KDM3A coincided with the loss of SIAH1 induction and the accumulation of dimethylated H3K9 surrounding the SIAH1 promoter. Interestingly, KDM3A expression was also up-regulated by nutritional stress in a HIF-1alpha dependent manner. PMID: 28843785
    2. Combination of netrin-1 and SIAH RNAi may prove to be a substantially effective therapy for myocardial infarction. PMID: 25561546
    3. The B23 regulates neuronal survival by preventing SIAH1-GAPDH death signaling under stress-induced conditions in the brain. PMID: 23027902
    4. The present study demonstrated for the first time that zygotic specific miRNA modulates the first cell cleavage through regulating expression of Siah1a. PMID: 22132158
    5. Siah1 protein is required for glucose limitation-induced Cyclin-Dependent Kinase Inhibitor p27 degradation. PMID: 21734459
    6. X ray structure: Siah ubiquitin ligase is structurally related to TRAF and modulates TNF-alpha signaling PMID: 11742346
    7. The ubiquitin ligase component Siah1a is required for completion of meiosis I in male mice PMID: 11884614
    8. Results from loss-of-function experiments do not support a general role for Siah genes in p53-mediated responses or mitosis. PMID: 12417719
    9. Results suggest that Dab1 inhibits the activity of Siah-1A. PMID: 12646221
    10. Results identify a striking low bone volume phenotype in Siah1a knockout mice, linking Siah1a to bone metabolism. PMID: 15123657
    11. Siah1A serves as a selective ubiquitin ligase that mediates ubiquitination-dependent degradation of long splice variants of group 1 mGluRs PMID: 15163799
    12. Siah1 is a binding partner for POSH (plenty of SH3s), a scaffold component of the apoptotic JNK pathway PMID: 16230351
    13. analysis of the substrate binding site of Siah ubiquitin ligase PMID: 16615911
    14. Siah1A (mouse) reduced PHD3 protein levels but Siah1A M180K (a substrate-binding groove mutant) did not alter the PHD3 protein levels suggesting that the Siah substrate-binding groove is important for PHD ubiquitylation and degradation. PMID: 18850011

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear.
  • 蛋白家族：
    SINA (Seven in absentia) family
  • 组织特异性：
    Widely expressed at low level in embryos and adults. Expressed at higher level in testis. Due to the high similarity between SIAH1A and SIAH1B, it is difficult to distinguish its own tissue specificity.
  • 数据库链接：

    KEGG: mmu:20437

    STRING: 10090.ENSMUSP00000044123

    UniGene: Mm.324553