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  4. Recombinant Mouse DNA repair and recombination protein RAD54-like (Rad54l)

Recombinant Mouse DNA repair and recombination protein RAD54-like (Rad54l)

  • 中文名称：
    小鼠Rad54l重组蛋白
  • 货号：
    CSB-YP019271MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Rad54l重组蛋白
  • 货号：
    CSB-EP019271MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Rad54l重组蛋白
  • 货号：
    CSB-EP019271MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Rad54l重组蛋白
  • 货号：
    CSB-BP019271MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Rad54l重组蛋白
  • 货号：
    CSB-MP019271MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rad54l
  • Uniprot No.：
  • 别名：
    Rad54l; Rad54; DNA repair and recombination protein RAD54-like; EC 3.6.4.-; RAD54 homolog; mHR54; mRAD54
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-747
  • 氨基酸序列
    MRRSLAPSQL ARRKPEDRSS DDEDWQPGTV TPKKRKSSSE TQVQECFLSP FRKPLTQLLN RPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL KRAGVRRALH DPLEEGALVL YEPPPLSAHD QLKLDKEKLP VHVVVDPILS KVLRPHQREG VKFLWECVTS RRIPGSHGCI MADEMGLGKT LQCITLMWTL LRQSPECKPE IEKAVVVSPS SLVKNWYNEV EKWLGGRIQP LAIDGGSKDE IDRKLEGFMN QRGARVPSPI LIISYETFRL HVGVLKKGNV GLVICDEGHR LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA HEFKKHFELP ILKSRDAAAS EADRQRGEER LRELIGIVNR CLIRRTSDIL SKYLPVKIEQ VVCCRLTPLQ TELYKRFLRQ AKPEEELREG KMSVSSLSSI TSLKKLCNHP ALIYDKCVAE EDGFEGTLGI FPPGYNSKAV EPQLSGKMLV LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRVRRYLY VRLDGTMSIK KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND EQAMARVWRD GQKKICYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ DVERHFSLGE LKELFTLDEA SLSDTHDRLH CRRCVNNRQV WPPPDGSDCT SDLAQWNHST DKRGLQDEVL QAAWDASSTA ITFVFHQRSH EEQRGLH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks. Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism. Plays also an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized. Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations.
  • 基因功能参考文献：
    1. Our study highlights the importance of the cooperation between Rad54 and Mus81 for mediating DNA DSB repair and restraining chromosome missegregation. PMID: 26876210
    2. Rad54 is not required for either transgene isotype switching or transgene hypermutation. PMID: 12218123
    3. Rad54-mediated homologous recombination is not essential for somatic hypermutation. In addition, the finding that the ablation of RAD51 paralogues causes an increase in SHM argues against a direct involvement of HR in promoting SHM. PMID: 12548566
    4. Rad54 has a role in telomere length maintenance PMID: 12897131
    5. Rad54 cooperates with DNA Ligase IV to support cellular proliferation, dna repair, and prevent chromosome and single chromatid aberrations PMID: 15175260
    6. Mus81-Eme1- and Rad54-mediated homologous recombination are involved in the same DNA replication-dependent interstrand crosslinks repair pathway PMID: 17036055
    7. These results reveal a differential interaction of the ATM-mediated DNA damage response and Rad54 paralog-mediated homologous recombination depending on the DNA damaging agent that initiates the response. PMID: 19061978

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 组织特异性：
    Hardly detectable in most tissues. Dramatically increased in thymus, spleen and testis.
  • 数据库链接：

    KEGG: mmu:19366

    STRING: 10090.ENSMUSP00000099765

    UniGene: Mm.3655