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  4. Recombinant Human parvovirus B19 Minor capsid protein VP1, partial

Recombinant Human parvovirus B19 Minor capsid protein VP1, partial

  • 中文名称：
    Recombinant Human parvovirus B19 Minor capsid protein VP1,partial,Yeast
  • 货号：
    CSB-YP362102HPM
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human parvovirus B19 Minor capsid protein VP1,partial,Yeast
  • 货号：
    CSB-EP362102HPM-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human parvovirus B19 Minor capsid protein VP1,partial,Yeast
  • 货号：
    CSB-MP362102HPM
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Minor capsid protein VP1; EC 3.1.1.4; Coat protein VP1
  • 种属：
    Human parvovirus B19 (isolate AU) (HPV B19)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have purchased your VP2 recombinant protein (cat#: CSB-MP362102HPM; Lot#: 03564) and I believes that the Glycerol in these proteins is interfering with their binding assay. I would like to buffer-exchange it to get rid of the Glycerol. Do you have any recommendations regarding the buffer to use in order to avoid problems with solubility? Perhaps use PBS? Lyophilized?

A:
Very nice to receive your inquiry. As to your question, pls check our reply as follows:
CSB-MP362102HPM Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 20% glycerol
As you can see, actually the glycerol concentration of these two items are already below our default shipping buffer 50% glycerol.
According to your feedback, since you already received these two items, does you want to change the buffer by yourself or need to order again ?
If you want to order again, we recommend you to choose the lyophilized form, the buffer before lyophilization will not contain any glycerol.
If you want to change the buffer or remove the glycerol by ourself, you can choose to remove the glycerol by dialysis or change the PBS buffer.
In addition, probably you can inform your application or requirements in advance next time, so that we can provide and supply the products according to his demand.
Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 10% glycerol
Q:

Thank you for the information provided below. I would like to know the extinction coefficient of CSB-MP362102HPM respectively? I will need to measure the concentration after the buffer exchange in case of loss.

A:
Very nice to receive your inquiry. CSB-MP362102HPM
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 91470
Abs 0.1% (=1 g/l) 1.504, assuming all pairs of Cys residues form cystines
Q:

1) Do you have any information on the stability of these protein below in PBS? I changed the buffer to PBS (got rid of glycerol) and stored them at -20C; do you think it may damage the proteins?
2) I may want to re-order and they would like to order in lyophilized format. I would like to know which buffer do you use to resolubilize the proteins? If the stability is not good after reconstitution, could it be possible to make aliquots?
3) Is it possible that I can order these tow proteins lyophilized and if I are not soluble following lyophilization then you can provide them in PBS?

A:
Very nice to receive your inquiry. 1.Sorry, I'm afraid we can't provide any useful information, since we haven't validated the stability in PBS buffer, But if your customer has already changed the buffer to PBS (got rid of glycerol) and stored them at -20C, will your customer use them after a long period ?
in theory, if the protein is already stable in it, this operation doesn't have any problem, and they will also be stable when stored at -20C.

2.Regarding resolubilizing the proteins, the reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins.
(Dissolve completely. The concentration could depend on customers' experiment demand.) It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted.
Generally, we recommend 0.1-1.0mg/ml concentration. If customer has special requirements for concentration, then they could dilute the protein according to their experiment demand.
If the stability is not good after reconstitution, could it be possible to make aliquots?
Yes, we can make aliquots according to your request, pls remark your requirement when placing the order.
3.Yes, we can provide these two proteins in lyophilized format, generally, we will do reconstitution test for all lyophilized proteins before the shipment, if we find they are not soluble following lyophilization, then we will provide them in liquid form. We can provide the protein with your desired buffer (PBS buffer), pls remark your requirement when placing the order.

靶点详情

  • 功能：
    Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 20 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Binds to erythroid progenitor cells expressing high levels of P antigen and uses host ITGA5-ITGB1 and XRCC5/Ku80 autoantigen as coreceptors on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region. The additional N-terminal region of isoform Minor capsid protein VP1, called VP1u, may serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.
  • 亚细胞定位：
    [Isoform Minor capsid protein VP1]: Virion. Host nucleus. Host cytoplasm.; [Isoform Major capsid protein VP2]: Virion. Host nucleus. Host cytoplasm.
  • 蛋白家族：
    Parvoviridae capsid protein family