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  4. Recombinant Human adenovirus E serotype 4 Fiber protein (L5)

Recombinant Human adenovirus E serotype 4 Fiber protein (L5)

  • 货号：
    CSB-YP336230HIQ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP336230HIQ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP336230HIQ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP336230HIQ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP336230HIQ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    L5
  • Uniprot No.：
  • 别名：
    L5; Fiber protein; SPIKE; Protein IV
  • 种属：
    Human adenovirus E serotype 4 (HAdV-4) (Human adenovirus 4)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-426
  • 氨基酸序列
    MSKSARGWSD GFDPVYPYDA DNDRPCPSST LPSFSSDGFQ EKPLGVLSLG PGRPCHTKNG EITLKLGEGV DLDDSGKLIA NTVNKAIAPL SFFQQHHFPL TWIPLYTPKM ENYPYKFLPP LSILKSTILN TLVSAFGSGL GLSGSALAVQ LASPLTFDDK GNIKITLNRG LHVTTGDAIE SNISWAKGIK FEDGAIATNI GKGSRFGTSS TETGVNNAYP IQVKLGSGLS FDSTGAIMAG NKDYDKLTLW TTPDPSPNCQ ILAENDAKLT LCLTMCDSQI LATVSVLVVR SGNLNPITGT VSSAQVFLRF DANGVLLTEH STSKKYWGYK QGDSIDGTPY TNAVGFMPNS TAYPKTQSST TKNNIVGQVY MNGDVSKPML LTITLNGTDD TTSAYSMSFS YTWTNGSYIG ATFGANSYTF SYIAQQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I would like to know more clarification between your following products.
1. Both CSB-BP320906HIL- CSB-MP320906HIL and CSB-BP336230HIQ- CSB-MP336230HIQ except some aa in the c-terminal. Is there any difference in protein stability, aggregation etc between them?
2.The fiber can be expressed from different hosts. Do you have gel picture of the purified fiber from different hosts or degree of glycosylation for different hosts?”
Can you please advice how to respond to customer about the difference if there is any.

A:
Thanks for your inquiry.
1.Recombinant Human adenovirus E serotype 4 Fiber protein(L5)
Expression Region: 1-426aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MSKSARGWSDGFDPVYPYDADNDRPCPSSTLPSFSSDGFQEKPLGVLSLGPGRPCHTKNGEITLKLGEGVDLDDSGKLIANTVNKAIAPLSFFQQHHFLTWIPLYTPKMENYPYKFLPPLSILKSTILNTLVSAFGSGLGLSGSALAVQLASPLTFDDKGNIKITLNRGLHVTTGDAIESNISWAKGIKFEDGAIATNIGKGSRFGTSSTETGVNNAYPIQVKLGSGLSFDSTGAIMAGNKDYDKLTLWTTPDPSPNCQILAENDAKLTLCLTMCDSQILATVSVLVVRSGNLNPITGTVSSAQVFLRFDANGVLLTEHSTSKKYWGYKQGDSIDGTPYTNAVGFMPNSTAYPKTQSSTTKNNIVGQVYMNGDVSKPMLLTITLNGTDDTTSAYSMSFSYTWTNGSYIGATFGANSYTFSYIAQQ


2.Recombinant Human adenovirus C serotype 5 Fiber protein(L5) ,partial
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPGVLSLRLSEPLVTSNGMLALKMGNGLSLDEAGNLTSQNVTTVSPPLKKTKSNINLEISAPLTVTSEALTVAAAAPLMVAGNTLTMQSQAPLTVHDSKLSIATQGPLTVSEGKLALQTSGPLTTTDSSTLTITASPPLTTATGSLGIDLKEPIYTQNGKLGLKYGAPLHVTDDLNTLTVATGPGVTINNTSLQTKVTGALGFDSQGNMQLNVAGGLRIDSQNRRLILDVSYPFDAQNQLNLRLGQGPLFINSAHNLDINYNKGLYLFTASNNSKKLEVNLSTAKGLMFDATAIAINAGDGLEFGSPNAPNTNPLKTKIGHGLEFDSNKAMVPKLGTGLSFDSTGAITVG


Pls feel free to contact us if you are interesed in some other expression regions, we will check if we can provide.
① You can find that the sequence of these two proteins are very different through the sequence comparison, the similarity is not high. See the sequence blast result below.
② Sorry, for the time being, we haven't expressed any of these proteins, so we can't provide the related gel picture of the purified fiber.
The main difference of the protein expressed by four different expression systems lie in the post-translational modification.
The protein expressed by E.coli expression system doesn't have post-translational modification(eg: glycosylation, phosphorylation, etc.),
and the other three systems can perform post-translational modifications, but the type and degree of modification are different.
The Yeast expression system have N-type and O-type glycosylation modification but its mechanism is different from that of higher eukaryotic expression systems,
The glycosylation modifications are mainly high mannose glycosylation modifications (such as yeast-specific O-mannosylation), lacking complex sugar chain modifications.
Baculovirus expression system has O-type glycosylation and high mannose N-type glycosylation, but it is generally not possible to perform peripheral glycosylation (eg, sialic acid N-glycosylation). The mammalian cells expression system has most thorough of glycosylation that can provide complex N-glycosylation and accurate O-glycosylation.

靶点详情

  • 功能：
    Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor CXCAR to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface.
  • 亚细胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Adenoviridae fiber family