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  4. Recombinant Human Transcription initiation factor IIA subunit 1 (GTF2A1)

Recombinant Human Transcription initiation factor IIA subunit 1 (GTF2A1)

  • 中文名称：
    人GTF2A1重组蛋白
  • 货号：
    CSB-YP009998HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GTF2A1重组蛋白
  • 货号：
    CSB-EP009998HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GTF2A1重组蛋白
  • 货号：
    CSB-EP009998HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GTF2A1重组蛋白
  • 货号：
    CSB-BP009998HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GTF2A1重组蛋白
  • 货号：
    CSB-MP009998HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GTF2A1
  • Uniprot No.：
  • 别名：
    General transcription factor IIA 1 19/37kDa; General transcription factor IIA subunit 1; General transcription factor IIA1; Glucose regulated protein 58kD pseudogene; gtf2a1; TF2A1; TF2AA_HUMAN; TFIIA 42; TFIIA alpha p55; TFIIA alpha p55, isoform 1; TFIIA p19 subunit; TFIIA p35 subunit; TFIIA-42; TFIIAL; Transcription initiation factor IIA beta chain; Transcription initiation factor IIA subunit 1; Transcription initiation factor TFIIA 42 kDa subunit
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    275-376
  • 氨基酸序列
    GTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENV VVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.
  • 基因功能参考文献：
    1. In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations. PMID: 28291257
    2. Data suggest that many TATA-box-containing promoters contain a TFIIA recognition element (IIARE) that activates transcription by increasing the recruitment of RNA polymerase II, TFIIA (TF2A1/TF2A2 subunits), TAF4, and P300. (TAF4 = TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1; P300 = E1A binding protein p300) PMID: 28539359
    3. The interaction of TBP-like protein with TFIIA is essential to suppress proteasome-dependent turnover of TBP-like protein PMID: 27696626
    4. Our results provide first evidence that Taspase1 processing affects TFIIA regulation of TFIID and suggest that Taspase1 processing of TFIIA is required to establish INR-selective core promoter activity in the presence of NC2. PMID: 25996597
    5. spatial positioning of the DNA-bound activation domain is important for efficient activation, possibly by maximizing its interactions with the transcriptional machinery including the TBP-TFIIA-TFIIB-promoter quaternary complex PMID: 12538582
    6. Results present the X-ray structures of human and yeast TATA box-binding protein /transcription factor IIA/DNA complexes at 2.1A and 1.9A resolution, respectively PMID: 12972251
    7. transcription factor IIA may regulate the intracellular molecular state and the function of TATA-binding protein-like protein (TLP) through its property of binding to TLP PMID: 14570910
    8. Transfected taspase 1 enhances cleavage of TFIIA, and RNA interference knockdown of endogenous taspase 1 diminishes cleavage of TFIIA in vivo. PMID: 16537915
    9. TFIIA induces a conformational change within the TBP/TATA complex that enhances its stability under both in vitro and physiological salt conditions. PMID: 17681538
    10. GTF2A1 alone, or GTF2A1 plus HAAO are excellent candidate biomarkers for detecting Ovarian cancer PMID: 19724865
    11. V96F substitution of a deamidated human GTF2A1 (TFIIA) peptide promotes T-cell activation in an in vitro model of Celiac disease inflammation. PMID: 12054857

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIA subunit 1 family
  • 数据库链接：

    HGNC: 4646

    OMIM: 600520

    KEGG: hsa:2957

    STRING: 9606.ENSP00000452454

    UniGene: Hs.592334