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  4. Recombinant Human Spindle and kinetochore-associated protein 1 (SKA1)

Recombinant Human Spindle and kinetochore-associated protein 1 (SKA1)

  • 中文名称：
    人SKA1重组蛋白
  • 货号：
    CSB-YP846589HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SKA1重组蛋白
  • 货号：
    CSB-EP846589HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SKA1重组蛋白
  • 货号：
    CSB-EP846589HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SKA1重组蛋白
  • 货号：
    CSB-BP846589HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SKA1重组蛋白
  • 货号：
    CSB-MP846589HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SKA1
  • Uniprot No.：
  • 别名：
    2810433K01Rik; AV117428; C18orf24; MGC10200; Ska1; SKA1_HUMAN; Spindle and kinetochore associated complex subunit 1; Spindle and kinetochore associated protein 1; Spindle and kinetochore-associated protein 1; Spindle and KT (kinetochore) associated 1; Spindle and KT associated 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-255
  • 氨基酸序列
    ASSDLEQLC SHVNEKIGNI KKTLSLRNCG QEPTLKTVLN KIGDEIIVIN ELLNKLELEI QYQEQTNNSL KELCESLEED YKDIEHLKEN VPSHLPQVTV TQSCVKGSDL DPEEPIKVEE PEPVKKPPKE QRSIKEMPFI TCDEFNGVPS YMKSRLTYNQ INDVIKEINK AVISKYKILH QPKKSMNSVT RNLYHRFIDE ETKDTKGRYF IVEADIKEFT TLKADKKFHV LLNILRHCRR LSEVRGGGLT RYVIT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
  • 基因功能参考文献：
    1. decreased neuronal cell SKA complex genes' expression levels affect neuronal cell viability and neurite development PMID: 29268205
    2. High SKA1 expression is associated with early recurrence and progression in patients with NMIBC, indicating SKA1 may serve as a promising prognostic biomarker for this disease. PMID: 29865039
    3. End-binding protein 1 (EB1) depletion results in a significant reduction of spindle and kinetochore-associated protein 1 (Ska1) recruitment onto microtubules and defects in mitotic chromosome alignment. PMID: 27225956
    4. SKA1 might be a promising target for cancer gene therapy in human ACC. PMID: 28340379
    5. Results show that SKA1 expression is regulated by mir-10a. Its knockdown inhibits migration and invasion in renal cell carcinoma cells. PMID: 28746769
    6. Study indicated that hypoxia mediated downregulation of SKA1 expression increased the chemotherapy resistance in human osteosarcoma cells. PMID: 28278080
    7. Kinetochores mature through Ska1/ska2/Ska3 complex recruitment and this is required for improved load-bearing capacity and silencing of the spindle assembly checkpoint. PMID: 28495837
    8. In conclusion, our findings suggest that spindle and kinetochore-associated protein 1 could serve as a potential therapeutic target in prostate cancer patients. PMID: 28651496
    9. expression positively associated with tumor-node-metastasis stage and recurrence of salivary adenoid cystic carcinoma PMID: 26881872
    10. Thus, the Ska complex, specifically the Ska1 C-terminal domain, recruits PP1 to kinetochores to oppose spindle checkpoint signaling kinases and promote anaphase onset. PMID: 26981768
    11. SKA1 is required for metastasis and cisplatin resistance of non-small cell lung cancer. PMID: 26985856
    12. knockdown of SKA1 could potently suppress bladder cancer cell proliferation in vitro and lentivirus-mediated silencing of SKA1 might serve as a novel strategy for gene therapy of bladder cancer. PMID: 26197237
    13. Results show that high SKA1 expression is predictive of poor prognosis of papillary thyroid carcinoma (PTC), implying it as a promising new target for PTC therapies. PMID: 26063960
    14. SKA1 could be used for gastric cancer early diagnosis as a biomarker. PMID: 24627241
    15. the kinetochore-associated Ska complex interacts with tubulin monomers via the carboxy-terminal winged-helix domain of Ska1, providing the structural basis for the ability to bind both straight and curved microtubule structures. PMID: 24413531
    16. Ska1 over-expression promotes tumourigenesis PMID: 24827423
    17. Data suggest that SKA1 plays an important role in the proliferation of hepatocellular carcinoma cells PMID: 24010405
    18. the conserved kinetochore-localized Ska1 complex tracks with depolymerizing microtubule ends and associates with both the microtubule lattice and curved protofilaments PMID: 23085020
    19. The structure of the Ska core complex is a W-shaped dimer of coiled coils, formed by intertwined interactions between Ska1, Ska2, and Ska3. PMID: 22483620
    20. Data suggest that Aurora B phosphorylation antagonizes the interaction between the Ska1-3 complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments. PMID: 22371557
    21. These data suggest that the Ska1/Ska2 complex plays a critical role in the maintenance of the metaphase plate and/or spindle checkpoint silencing. PMID: 17093495
    22. The full Ska1 complex forms assemblies on microtubules that can facilitate the processive movement of microspheres along depolymerizing microtubules. PMID: 19289083

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    SKA1 family
  • 数据库链接：

    HGNC: 28109

    OMIM: 616673

    KEGG: hsa:220134

    STRING: 9606.ENSP00000285116

    UniGene: Hs.134726