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  4. Recombinant Human Protein chibby homolog 1 (CBY1)

Recombinant Human Protein chibby homolog 1 (CBY1)

  • 中文名称：
    Recombinant Human Protein chibby homolog 1(CBY1)
  • 货号：
    CSB-EP896889HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    CBY1
  • Uniprot No.：
  • 别名：
    ARPP-binding protein; C22orf2; CBY; Cby1; CBY1_HUMAN; Cytosolic leucine rich protein ; Cytosolic leucine-rich protein; Golgi and endoplasmic reticulum-associated 1; HRIHFB2025; PIGEA 14; PIGEA-14; PKD2 interactor; PKD2 interactor, Golgi and endoplasmic reticulum-associated 1; Protein Chibby; Protein chibby homolog 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    41.5kDa
  • 表达区域：
    1-126aa
  • 氨基酸序列
    MPFFGNTFSPKKTPPRKSASLSNLHSLDRSTREVELGLEYGSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibits the Wnt/Wingless pathway by binding to CTNNB1/beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.
  • 基因功能参考文献：
    1. FAM92 proteins interact with Cby1 to promote ciliogenesis via regulation of membrane-remodeling processes. PMID: 27528616
    2. chronic Cby1 knockdown in colon cancer cells may counteract tumor progression by promoting the mesenchymal-to-epithelial transition process. PMID: 28107095
    3. Cby's C-terminal domain alone binds to TC-1 with significantly greater affinity compared to full-length Cby, implying that target binding of the coiled-coil domain is affected by the flanking disordered regions. PMID: 27082063
    4. CBY1 downregulation in CML comes from reduced protein stability when bound to 14-3-3sigma adapter protein. Dissociation raises CBY1 protein levels by enhancing its stability. The ubiquitin proteasome system reduces 14-3-3sigma-bound CBY1 stability by SUMOylation. PMID: 26147002
    5. Study showed that expression of Cby protein was strongly downregulated in laryngeal squamous cell carcinoma (LSCC) tumor tissues in comparison to normal laryngeal mucosa samples. PMID: 25175341
    6. the impact of BCR-ABL1 on Chibby1, a beta catenin antagonist involved in cell differentiation and transformation PMID: 24339928
    7. Stable expression of CBY1 drives beta catenin cytoplasmatic translocation and impairs beta catenin signaling in BCR-ABL + cells. PMID: 23707389
    8. Results from the association analysis suggest that common variation in CBY is not a cause for obesity in the Belgian population. PMID: 23645032
    9. C-terminal polycystin-2 influences the interaction with PIGEA14 PMID: 23838289
    10. Cby plays an important role in organization of both primary and motile cilia in collaboration with Cnx. PMID: 22911743
    11. The N-terminal portion of the CBY1 is unstructured in solution, but the C-terminal half forms a coiled-coil structure. PMID: 21182262
    12. Chibby and clusterin were co-immunoprecipitated with NBPF1. PMID: 20096688
    13. These findings unravel the molecular basis through which a combinatorial action of Cby and 14-3-3 proteins controls the dynamic nuclear-cytoplasmic trafficking of beta-catenin. PMID: 19940019
    14. Chibby inhibits beta-catenin-mediated transcriptional activation by competing with Lef-1 to bind to beta-catenin PMID: 12712206
    15. PIGEA-14 plays an important role in regulating the intracellular location of polycystin-2 PMID: 15194699
    16. As no somatic mutation was detected in C22orf2 in 36 colorectal tumour DNA, our results do not support the implication of Chibby as a tumour suppressor in colorectal carcinogenesis. PMID: 15245581
    17. Chibby is not likely to promote colorectal carcinoma tumor development or progression. PMID: 16570344
    18. CBY promotes adipocyte differentiation through inhibition of beta catenin signaling. PMID: 17403895
    19. intrinsically disordered TC-1 interacts with Cby via its transient helical structure PMID: 17905836
    20. 14-3-3epsilon and 14-3-3zeta are identified as Cby-binding partners. PMID: 18573912
    21. genes including CHIBBY involved in pediatric ependymomas. PMID: 18663750
    22. Alanine substitutions of two or more of four critical leucine residues within the C-terminal heptad repeats eliminate the Cby-Cby interaction. PMID: 19435523

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  • 亚细胞定位：
    Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus. Golgi apparatus, trans-Golgi network.
  • 蛋白家族：
    Chibby family
  • 组织特异性：
    Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors.
  • 数据库链接：

    HGNC: 1307

    OMIM: 607757

    KEGG: hsa:25776

    STRING: 9606.ENSP00000216029

    UniGene: Hs.334911