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  4. Recombinant Human Nitric oxide synthase-interacting protein (NOSIP)

Recombinant Human Nitric oxide synthase-interacting protein (NOSIP)

  • 中文名称：
    人NOSIP重组蛋白
  • 货号：
    CSB-YP897477HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NOSIP重组蛋白
  • 货号：
    CSB-EP897477HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NOSIP重组蛋白
  • 货号：
    CSB-EP897477HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NOSIP重组蛋白
  • 货号：
    CSB-BP897477HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NOSIP重组蛋白
  • 货号：
    CSB-MP897477HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NOSIP
  • Uniprot No.：
  • 别名：
    NOSIP; CGI-25; Nitric oxide synthase-interacting protein; E3 ubiquitin-protein ligase NOSIP; EC 2.3.2.27; RING-type E3 ubiquitin transferase NOSIP; eNOS-interacting protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-301
  • 氨基酸序列
    MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTP DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR PSGAVVTLEC VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB. Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.
  • 基因功能参考文献：
    1. While NOSIP expression was increased in Hirschsprung's disease (HSCR) vs. non- HSCR patients, no significant difference was observed in patients with Hirschsprung's associated enterocolitis. The increased expression of NOSIP in the aganglionic and ganglionic bowel of HSCR may contribute to the development of enterocolitis by inhibiting local nitric oxide production in patients with Hirschsprung's disease. PMID: 28196663
    2. Endogenous NOSIP reduces the enzymatic capacity of eNOS, specifically in the G(2) phase of the cell cycle by targeting eNOS to the actin cytoskeleton. PMID: 16135813
    3. endothelial nitric oxide synthase (eNOS) interacting protein (NOSIP), modulator of eNOS activity by alteration of eNOS subcellular localisation; high expression levels in endothelial cells and vascularized tissue. PMID: 11149895
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813).
  • 蛋白家族：
    NOSIP family
  • 组织特异性：
    Expressed in heart, brain and lung. Present in endothelial cells (at protein level).
  • 数据库链接：

    HGNC: 17946

    OMIM: 616759

    KEGG: hsa:51070

    STRING: 9606.ENSP00000375726

    UniGene: Hs.7236