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  4. Recombinant Human Melanoma antigen recognized by T-cells 1 (MLANA)

Recombinant Human Melanoma antigen recognized by T-cells 1 (MLANA)

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  • 中文名称：
    人MLANA重组蛋白
  • 货号：
    CSB-EP621968HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Antigen LB39 AA; Antigen LB39-AA; Antigen SK29 AA; Antigen SK29-AA; MAR1_HUMAN; MART 1; MART-1; MART1; Melan A; Melan A protein; Melanoma antigen recognized by T cells 1; Melanoma antigen recognized by T-cells 1; MLAN A; MLANA; OTTHUMP00000021036; OTTHUMP00000021037; OTTHUMP00000021038; Protein Melan-A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    27.2kDa
  • 表达区域：
    1-118aa
  • 氨基酸序列
    MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-B2M-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
  • 基因功能参考文献：
    1. Melan-A-stained slides shows definitive invasion. PMID: 27533878
    2. Report automated quantification of proliferation with automated hot-spot selection in phosphohistone H3/MART1 dual-stained stage I/II melanoma. PMID: 27062658
    3. Suggest MelanA-negative spindle-cell associated melanoma constitutes a distinct inflammatory phenotype correlated with dense infiltration of CD163 macrophages and loss of E-cadherin. PMID: 25602697
    4. Inhibin alpha-subunit, Melan A and MNF116 were not sensitive for pheochromocytomas. PMID: 25329118
    5. the presence of antibody and T-cell immune responses directed against the melanocyte-differentiation-antigens MART-1 (Melan-A), tyrosinase and gp100 in patients with melanoma-associated leucoderma, as compared to patients with vitiligo. PMID: 25043574
    6. Knockdown of T-bet expression in Mart-127-35 -specific T-cell-receptor-engineered human CD4(+) CD25(-) and CD8(+) T cells attenuates effector function. PMID: 25495780
    7. Melan-A was useful to confirm the diagnosis of lentigo maligna in early lesions and to differentiate these from chronically sun-damaged skin. PMID: 24394300
    8. Misinitiation of intrathymic MART-1 transcription and biased TCR usage explain the high frequency of MART-1-specific T cells. PMID: 24846220
    9. Comprehensive characterization of the marginal and joint distributions for S100, HMB-45, and Melan-A across a large series of cutaneous melanomas revealed diversity of expression across this group of antigens. PMID: 23899062
    10. melan A (A103) is not expressed in mesotheliomas PMID: 22820661
    11. Report diagnostic role of immunohistochemical staining for Melan-A in evaluating primary melanoma and, specifically, in situ melanoma cases. PMID: 22688397
    12. In this study, production of the C terminus of the Melan-A(26-35) epitope requires cleavage within a hydrophobic stretch, after V35. Hence, the standard proteasome is the only proteasome able to produce the C terminus of this epitope. PMID: 22925930
    13. naturally occurring MART-1 melanoma antigen can be taken-up from dying melanoma cells into DC or MAK and both cell types can induce specific CD8(+) T cell cross-presentation thereafter. PMID: 22768350
    14. The presence of circulating T cells responding to Melan-A or NY-ESO-1 had strong independent prognostic impact on survival in advanced melanoma. PMID: 22529253
    15. The modified melan-A long peptides (not their wild-type counterparts) give rise to highly sustained cross-presentation capacity by monocyte-derived dendritic cells and could be used as tumor vaccines for treatment of melanoma patients. PMID: 22291187
    16. Aberrant expression of Melan-A and MART-1 in AFX and undifferentiated pleomorphic sarcoma of the skin represents an important diagnostic pitfall with potential for misdiagnosis as melanoma. PMID: 22050092
    17. MART-1- and gp100-expressing and -non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro. PMID: 21993558
    18. we established several CD4 T-cell clones that recognized a peptide that is embedded within Melan-A/MART-121-50, in a HLA-DPB1*0501 restricted manner PMID: 21760531
    19. Melanocyte MART-1 may correlate to the autoimmune mechanism in children with vitiligo. PMID: 19861278
    20. The differential expression of Mart-1 was investigated in four human uveal melanoma cells. PMID: 21667026
    21. Translocation of the BCL2 gene on the chromosome band 18q21.3 and MYC translocation to 14q32/IGH or less commonly to 2p11/IGK in an identical cell is termed BCL2 and MYC dual-hit lymphoma/leukemia PMID: 21362015
    22. Both semiscale and fullscale quantitative analyses of Ki67/MART1 stains are valuable diagnostic tools to distinguish melanomas and nevi with a large degree of certainty. PMID: 21610457
    23. Molecular upstaging of MART-1 using rt-pcr was correlated with prognosis in melanoma patients PMID: 21135695
    24. determined the crystal structure of a phosphopeptide derived from melanoma antigen recognized by T cells-1 (pMART-1), selectively expressed by melanomas, in complex with HLA-DR1 PMID: 20417641
    25. OA1 interacts with MART-1 at early stages of melanogenesis to control melanosome identity and composition. PMID: 19717472
    26. unexpected high frequency of T cells specific for the self-antigen Melan-A/MART-1 in CD8 single-positive thymocytes from human histocompatibility leukocyte antigen-A2 healthy individuals PMID: 11854361
    27. Melanosomes from an Achilles tendon clear-cell sarcoma showed expression of the Melan-A gene. PMID: 12072203
    28. The high frequency of Melan-A multimer(+) T cells can be explained by the existence of largely cross-reactive subsets of naive CD8(+) T cells displaying multiple specificities. PMID: 12119345
    29. dominant function of TCRAV2 segment in forming the TCR repertoire specific for the human self Ag Melan-A/MART-1 PMID: 12444131
    30. Data show that MITF appears to regulate the expression of the SILV and MLANA genes. PMID: 12819038
    31. Antigen-specific T lymphocyte reactivity to MelanA/MART-1 and tyrosinase peptides was not observed ex vivo in our vitiligo patients, and only one patient demonstrated responses to MelanA/MART-1 PMID: 12925214
    32. adrenocortical neoplasms showed strong and diffuse granular cytoplasmic staining for Melan A. No nuclear reaction was observed. PMID: 14663359
    33. the expression of Melan-A/MART-1 in patient-derived cell cultures may help to identify a group of melanoma patients with prolonged survival PMID: 15305155
    34. Melan-A is very useful in distinguishing between desmoplastic melanoma and sclerotic nevi. PMID: 15618925
    35. Data suggest that MART-1 is indispensable for Pmel17 function and thus plays an important role in regulating mammalian pigmentation. PMID: 15695812
    36. Two new Melan-A-derived CD4+ T cell epitopes have been identified that map to the 1-20 and 91-110 regions of the protein and are restricted by HLA-DR11 and HLA-DR52 molecules. PMID: 16814609
    37. Blocking MAPK signaling augments antigen levels, thereby enhancing Melan-A/MART-1-specific cytotoxic T-cell responses to antigen-negative cells following MEK inhibition treatment. PMID: 17050671
    38. Two novel Melan-A/MART-1-derived sequences recognized by CD4 T cells from melanoma patients provide the basis for monitoring of naturally occurring and vaccine-induced Melan-A/MART-1-specific CD4 T cell responses. PMID: 17082590
    39. HMB-45 and Melan-A combined were positive in 100% of the renal angiomyolipomas. PMID: 17227112
    40. Data show that the MAT-1 26-35 and 27-35 peptides adopt strikingly different conformations when bound to HLA-A2. PMID: 17719062
    41. t cell recaptor antigens and the major histocompatibilyt complex are complexed with this protein. PMID: 17768347
    42. the combinational characterization of calretinin (either by polyclonal or monoclonal antibody), inhibin alpha, and Melan-A expression is of great significance in the differential diagnosis of adrenocortical tumors. PMID: 18091323
    43. these data significantly enlarge the fraction of melanoma patients susceptible to benefit from a Melan-A/MART1 vaccine approach. PMID: 18097665
    44. Measurement of Melan-A, gp100, MAGE-3, MIA and tyrosinase represents a prognostic factor and a method for early detection of metastasis and treatment response of melanoma patients. PMID: 18181974
    45. Although melan-A-specific T cells were detectable, they are unlikely to be involved in the etiopathogenesis of vitiligo. PMID: 18337837
    46. Increased staining with Melan-A, in an actinic keratosis, or solar lentigo should raise the possibility of a contiguous melanoma in situ. PMID: 18494818
    47. results show a complex pattern of changes in the expression of Melan-A in melanomas depending on the location of melanoma cells within individual skin layers PMID: 18626310
    48. the generation of larger numbers of ELAGIGILTV ((MART-1 26-35, A27L)-specific CD8+ T cells correlates negatively with the acquisition of a CD45RA+CCR7- phenotype and cytotoxic capacity. PMID: 19050104
    49. T-cell receptor beta chain sequences of Melan-A-specific clonotypes obtained from melanoma patients were highly heterogeneous, but displayed a preferential usage of few TRBV and TRBJ segments. PMID: 19317896
    50. Case Report: Melan-a-positive "pseudomelanocytic nests": a pitfall in the histopathologic and immunohistochemical diagnosis of pigmented lesions on sun-damaged skin. PMID: 19384076

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
  • 组织特异性：
    Expression is restricted to melanoma and melanocyte cell lines and retina.
  • 数据库链接：

    HGNC: 7124

    OMIM: 605513

    KEGG: hsa:2315

    STRING: 9606.ENSP00000370880

    UniGene: Hs.154069