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  4. Recombinant Human Kinetochore protein Spc25 (SPC25)

Recombinant Human Kinetochore protein Spc25 (SPC25)

  • 中文名称：
    人SPC25重组蛋白
  • 货号：
    CSB-YP881019HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SPC25重组蛋白
  • 货号：
    CSB-EP881019HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SPC25重组蛋白
  • 货号：
    CSB-EP881019HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SPC25重组蛋白
  • 货号：
    CSB-BP881019HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SPC25重组蛋白
  • 货号：
    CSB-MP881019HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPC25
  • Uniprot No.：
  • 别名：
    AD024; hSpc25; Kinetochore protein Spc25; SPBC25; SPC25; SPC25_HUMAN; spindle pole body component 25 homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-224
  • 氨基酸序列
    MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN VRKTNNFSAF LANVRKAFTA TVYN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
  • 基因功能参考文献：
    1. SPC25 overexpression significantly increased the cancer stem cell properties and invasion of A549 cells. PMID: 29432994
    2. SPC25 expressed by extracellular matrix stiffening is required for lung cancer cell proliferation. PMID: 29709477
    3. SPC25 overexpression is associated with Breast Cancer. PMID: 27197203
    4. hSPC25 is an essential kinetochore component that plays a significant role in proper execution of mitotic events PMID: 14699129
    5. the Spc24, Spc25, Nuf2, and Ndc80/Hec1 complex is a faithful copy of the endogenous Ndc80 complex PMID: 15961401

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    SPC25 family
  • 数据库链接：

    HGNC: 24031

    OMIM: 609395

    KEGG: hsa:57405

    STRING: 9606.ENSP00000282074

    UniGene: Hs.421956