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  4. Recombinant Human Kallikrein-12 (KLK12)

Recombinant Human Kallikrein-12 (KLK12)

  • 中文名称：
    人KLK12重组蛋白
  • 货号：
    CSB-YP892137HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人KLK12重组蛋白
  • 货号：
    CSB-EP892137HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人KLK12重组蛋白
  • 货号：
    CSB-BP892137HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人KLK12重组蛋白
  • 货号：
    CSB-MP892137HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KLK12
  • Uniprot No.：
  • 别名：
    KLK12; KLKL5; UNQ669/PRO1303Kallikrein-12; EC 3.4.21.-; Kallikrein-like protein 5; KLK-L5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    18-248
  • 氨基酸序列
    ATP KIFNGTECGR NSQPWQVGLF EGTSLRCGGV LIDHRWVLTA AHCSGSRYWV RLGEHSLSQL DWTEQIRHSG FSVTHPGYLG ASTSHEHDLR LLRLRLPVRV TSSVQPLPLP NDCATAGTEC HVSGWGITNH PRNPFPDLLQ CLNLSIVSHA TCHGVYPGRI TSNMVCAGGV PGQDACQGDS GGPLVCGGVL QGLVSWGSVG PCGQDGIPGV YTYICKYVDW IRMIMRNN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP892137HU, could you please provide some informatins for all available sizes?
Additionally, do you know if any customers regularly order this protein, or if any of the expression hosts are expected to produce to have activity?

A:
Thanks for your inquiry.
Expression Region:18-248aa; Mature full length protein.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

ATPKIFNGTECGRNSQPWQVGLFEGTSLRCGGVLIDHRWVLTAAHCSGSRYWVRLGEHSLSQLDWTEQIRHSGFSVTHPGYLGASTSHEHDLRLLRLRLPVRVTSSVQPLPLPNDCATAGTECHVSGWGITNHPRNPFPDLLQCLNLSIVSHATCHGVYPGRITSNMVCAGGVPGQDACQGDSGGPLVCGGVLQGLVSWGSVGPCGQDGIPGVYTYICKYVDWIRMIMRNN


Sorry, we haven't expressed this protein up to now, and haven't validated the activity, so we can't guarantee that it's 100% active.
However, what we provide is the mature full length protein, and it's purified under very mild condition, in theory, it has great potential to be active.We preferentially recommend the protein expressed by the eukaryotic expression system.
In addition,an attached literature introduces that this protein expressed by the mammalian cell expression system is active.

靶点详情

  • 基因功能参考文献：
    1. results demonstrate the discriminative value of KLK12sv1/2 and KLK12sv3 between benign and malignant breast tumors as well as their potential favorable prognostic significance in breast adenocarcinoma. PMID: 29807016
    2. KLK12 gene silencing reduces gastric cancer cell proliferation and migration. PMID: 27706634
    3. Data indicate that the KLK12 SNP rs3865443 was not associated with tumor aggressiveness but showed marginal association with prostate cancer risk for the rare homozygote. PMID: 21741862
    4. KLK12 gene is markedly overexpressed in gastric cancer PMID: 23236234
    5. the proangiogenic activity of KLK12 in lung endothelial cells was not related to a kinin release PMID: 23152405
    6. Results suggest that kallikrein-related peptidase 12 (KLK12) splice variant KLK12sv3 can be regarded as a marker of good prognosis in breast cancer. PMID: 22351561
    7. KLK12 may indirectly regulate the bioavailability and activity of several growth factors through processing of their CCN binding partners PMID: 21628462
    8. 4 types of polymorphisms were found in Japanese gastric cancer: 1 at an intron 4 splice-donor site (c.457+2T>C), 2 in exon 6 (c.618_619delTG:p.Cys206fsX72 & c.735G>A:p.Met245Ile), & 1 in intron 3. c.457+2C/C has no hK12 serine protease expression. PMID: 15300858
    9. KLK12 has trypsin-like activity, cleaving peptide bonds after both arginine & lysine. It quickly loses its activity due to autodegradation, its activity can also be rapidly inhibited by zinc ions & by alpha2-antiplasmin through covalent complex formation. PMID: 17391064

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Kallikrein subfamily
  • 数据库链接：

    HGNC: 6360

    OMIM: 605539

    KEGG: hsa:43849

    STRING: 9606.ENSP00000250351

    UniGene: Hs.411572