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  4. Recombinant Human Huntingtin-associated protein 1 (HAP1)

Recombinant Human Huntingtin-associated protein 1 (HAP1)

  • 中文名称：
    人HAP1重组蛋白
  • 货号：
    CSB-YP010129HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人HAP1重组蛋白
  • 货号：
    CSB-EP010129HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人HAP1重组蛋白
  • 货号：
    CSB-EP010129HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人HAP1重组蛋白
  • 货号：
    CSB-BP010129HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人HAP1重组蛋白
  • 货号：
    CSB-MP010129HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HAP1
  • Uniprot No.：
  • 别名：
    HAP 1; HAP 1A; HAP 2; HAP-1; Hap1; HAP1_HUMAN; HAP2; HAPP; hHAP1; hHLP1; HIP 5; HIP5; HLP; HLP1; Huntingtin associated protein 1A; Huntingtin-associated protein 1; huntingtin-associated protein 2; Neuroan 1; Neuroan1; OTTHUMP00000164746; OTTHUMP00000164747; OTTHUMP00000232821; OTTHUMP00000232822
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-671
  • 氨基酸序列
    MRPKRLGRCC AGSRLGPGDP AALTCAPSPS ASPAPEPSAQ PQARGTGQRV GSRATSGSQF LSEARTGARP ASEAGAKAGA RRPSAFSAIQ GDVRSMPDNS DAPWTRFVFQ GPFGSRATGR GTGKAAGIWK TPAAYVGRRP GVSGPERAAF IRELEEALCP NLPPPVKKIT QEDVKVMLYL LEELLPPVWE SVTYGMVLQR ERDLNTAARI GQSLVKQNSV LMEENSKLEA LLGSAKEEIL YLRHQVNLRD ELLQLYSDSD EEDEDEEEEE EEKEAEEEQE EEEAEEDLQC AHPCDAPKLI SQEALLHQHH CPQLEALQEK LRLLEEENHQ LREEASQLDT LEDEEQMLIL ECVEQFSEAS QQMAELSEVL VLRLENYERQ QQEVARLQAQ VLKLQQRCRM YGAETEKLQK QLASEKEIQM QLQEESVWVG SQLQDLREKY MDCGGMLIEM QEEVKTLRQQ PPVSTGSATH YPYSVPLETL PGFQETLAEE LRTSLRRMIS DPVYFMERNY EMPRGDTSSL RYDFRYSEDR EQVRGFEAEE GLMLAADIMR GEDFTPAEEF VPQEELGAAK KVPAEEGVME EAELVSEETE GWEEVELELD EATRMNVVTS ALEASGLGPS HLDMNYVLQQ LANWQDAHYR RQLRWKMLQK GECPHGALPA ASRTSCRSSC R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion; the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to facilitate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptors to synapses; the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesis. Involved in regulation of exocytosis. Seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs; sequestration is enhanced by an expanded polyglutamine repeat within TBP. HAP1-containing STBs have been proposed to play a protective role against neurodegeneration in Huntigton disease (HD) and spinocerebellar ataxia 17 (SCA17).
  • 基因功能参考文献：
    1. HAP1 is expressed in endocrine cells of the human gut. PMID: 27737633
    2. data fully support that HAP1 is a GKAP, anchoring specifically to the cGMP-dependent protein kinase isoform Ibeta, and provide further evidence that also PKG spatiotemporal signaling is largely controlled by anchoring proteins PMID: 25653285
    3. The -141 T > G polymorphism, but not the 1349 T > G polymorphism, may have protective effects for lung cancer. PMID: 25081373
    4. HAP1 gene expression is related to the radiosensitivity of breast cancer cells and may play an important role in the regulation of cellular radiosensitivity PMID: 25446120
    5. Overexpression of HAP1 reduced in vitro cell growth in breast cancer cell lines. PMID: 23440330
    6. The results of this study found no association was found between the HAP1 T441M polymorphism and the age at onset of Huntington's disease . PMID: 22698993
    7. The results of this study suggested that HAP1 co-localizes and associates with APP in physiological conditions of mouse and human brain. PMID: 22731248
    8. WT HTT regulates ciliogenesis by interacting through huntingtin-associated protein 1 (HAP1) with pericentriolar material 1 protein (PCM1). PMID: 21985783
    9. HAP1/stigmoid body interacts with the normal ataxin-3 through Josephin domain PMID: 21386698
    10. sortilin stabilizes the proBDNF.HAP1 complex PMID: 21357693
    11. ADORA2A, but not HAP1 or OGG1, may have a role in age at onset in Huntington's disease PMID: 20512606
    12. REVIEW: function of HAP1 PMID: 19262167
    13. HAP1 may participate in axonal transport and activity-dependent release of pro-BDNF by interacting with the BDNF prodomain. PMID: 19996106
    14. The combination of siRNA, the SB transposon, and an accurate transgenic mouse model may permit evaluation of this approach in preventing the pathogenesis associated with expression of mutant Htt. PMID: 15737634
    15. Study provides genetic and functional evidence that the M441-HAP1 polymorphism modifies the age-at-onset of Huntington's disease . PMID: 18192679
    16. REST/NRSF, dynactin p150(Glued), huntingtin, HAP1, and RILP form a complex involved in the translocation of REST/NRSF into the nucleus and HAP1 controls REST/NRSF cellular localization in neurons PMID: 18922795

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  • 亚细胞定位：
    Cytoplasm. Cell projection, axon. Cell junction, synapse, presynapse. Cytoplasm, cytoskeleton. Cell projection, dendritic spine. Cell projection, dendrite. Lysosome. Endoplasmic reticulum. Mitochondrion. Nucleus. Cytoplasmic vesicle, autophagosome. Early endosome. Cell projection, growth cone. Cell projection, neuron projection. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
  • 组织特异性：
    Predominantly expressed in brain. Selectively expressed in neurons.
  • 数据库链接：

    HGNC: 4812

    OMIM: 600947

    KEGG: hsa:9001

    UniGene: Hs.158300