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  4. Recombinant Human Granzyme M (GZMM)

Recombinant Human Granzyme M (GZMM)

  • 中文名称：
    人GZMM重组蛋白
  • 货号：
    CSB-YP010085HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GZMM重组蛋白
  • 货号：
    CSB-EP010085HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GZMM重组蛋白
  • 货号：
    CSB-EP010085HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GZMM重组蛋白
  • 货号：
    CSB-BP010085HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GZMM重组蛋白
  • 货号：
    CSB-MP010085HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GZMM
  • Uniprot No.：
  • 别名：
    GRAM_HUMAN; Granzyme M (lymphocyte met ase 1); Granzyme M; GranzymeM; GZM M; Gzmm; HU Met 1; HU-Met-1; LMET 1; LMET1; Lymphocyte met ase 1; MET 1; Met 1 serine protease; Met ase; Met-1 serine protease; Met-ase; MET1; Met1 serine protease; Natural killer cell granular protease
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-257
  • 氨基酸序列
    IIGGR EVIPHSRPYM ASLQRNGSHL CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR KVTGRSA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.
  • 基因功能参考文献：
    1. GzMM has a critical role during early stages of inflammation in ulcerative colitis; in its absence colonic inflammation is enhanced. PMID: 27441655
    2. We also report that TNFalpha induces the attachment of Met1-linked Ub chains directly to TNF receptor 1, which do not seem to be attached covalently to Lys63-linked or other types of ubiquitin chain. PMID: 27133719
    3. Granzym M may be important in regulating HCMV latency and reactivation in SCT patients. PMID: 24316590
    4. GrM is expressed in HCMV-specific CD8thorn T cells and cleaves host cell protein hnRNP K in the presence of RNA. PMID: 23099853
    5. FADD cleavage by NK cell granzyme M enhances its self-association to facilitate procaspase-8 recruitment for auto-processing leading to caspase cascade. PMID: 21979465
    6. Results indicate that cellular overexpression of SERPINB4 inhibits recombinant GrM-induced as well as NK cell-mediated cell death. PMID: 21857942
    7. Results describe the structural characterization of granzyme M by molecular modeling as well as by detailed comparison with other granzymes. PMID: 20107908
    8. Noncytotoxic inhibition of cytomegalovirus replication through NK cell protease granzyme M-mediated cleavage of viral phosphoprotein 71. PMID: 21059895
    9. survivin cleavage by Granzyme M triggers degradation of the survivin-X-linked inhibitor of apoptosis protein (XIAP) complex to free caspase activity leading to cytolysis of target tumor cells PMID: 20406824
    10. These findings support not only a role for GrM in the innate but also in the adaptive immune response. PMID: 19896187
    11. GM expression is a distinctive feature of the nasal NK/T-cell, gamma delta T-cell, and intestinal T-cell lymphomas, and suggest that these tumors develop from lymphocytes involved in innate immunity. PMID: 12506019
    12. granzyme M represents a third major and specialized perforin-dependent cell death pathway that plays a significant role in death mediated by NK cells PMID: 15028722
    13. Granzyme (Gzm)M can directly degrade inhibitor of caspase-activated DNase (ICAD) to activate CAD, leading to DNA damage; GzmM cleaves DNA damage sensor enzyme poly(ADP-ribose) polymerase to prevent cellular DNA repair and force apoptosis. PMID: 16818775
    14. Granzyme M targets major components of the cytoskeleton that likely contribute to natural killer (NK) cell-induced cell death. PMID: 18523284
    15. NPM is essential for cell viability, these data suggest that targeting of NPM by granzyme M may contribute to tumor cell eradication by abolishing NPM function. PMID: 19103589
    16. Structure-based mutagenesis reveals that the amino-terminus and catalytic triad of GzmM are most essential for proteolytic function. PMID: 19542453

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  • 亚细胞定位：
    Secreted. Cytoplasmic granule. Note=Granules of large granular lymphocytes.
  • 蛋白家族：
    Peptidase S1 family, Granzyme subfamily
  • 组织特异性：
    Highly and constitutively expressed in activated natural killer (NK) cells.
  • 数据库链接：

    HGNC: 4712

    OMIM: 600311

    KEGG: hsa:3004

    STRING: 9606.ENSP00000264553

    UniGene: Hs.465511