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  4. Recombinant Human Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC)

Recombinant Human Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC)

  • 中文名称：
    人GOPC重组蛋白
  • 货号：
    CSB-YP862075HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人GOPC重组蛋白
  • 货号：
    CSB-EP862075HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人GOPC重组蛋白
  • 货号：
    CSB-EP862075HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人GOPC重组蛋白
  • 货号：
    CSB-BP862075HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人GOPC重组蛋白
  • 货号：
    CSB-MP862075HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GOPC
  • Uniprot No.：
  • 别名：
    CAL; CFTR associated ligand; CFTR-associated ligand; dJ94G16.2; dJ94G16.2 PIST; FIG; Fused in glioblastoma; Golgi associated PDZ and coiled coil motif containing; Golgi associated PDZ and coiled coil motif containing protein; Golgi-associated PDZ and coiled-coil motif-containing protein; GOPC 1; GOPC; GOPC_HUMAN; GOPC1; OTTHUMP00000040403; PDZ protein interacting specifically with TC 10; PDZ protein interacting specifically with TC10; PDZ/coiled coil domain binding partner for the rho family GTPase TC 10; PDZ/coiled coil domain binding partner for the rho family GTPase TC10; PIST; Protein interacting specifically with Tc 10; Protein interacting specifically with Tc10
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-462
  • 氨基酸序列
    SAGGPCPAA AGGGPGGASC SVGAPGGVSM FRWLEVLEKE FDKAFVDVDL LLGEIDPDQA DITYEGRQKM TSLSSCFAQL CHKAQSVSQI NHKLEAQLVD LKSELTETQA EKVVLEKEVH DQLLQLHSIQ LQLHAKTGQS ADSGTIKAKL SGPSVEELER ELEANKKEKM KEAQLEAEVK LLRKENEALR RHIAVLQAEV YGARLAAKYL DKELAGRVQQ IQLLGRDMKG PAHDKLWNQL EAEIHLHRHK TVIRACRGRN DLKRPMQAPP GHDQDSLKKS QGVGPIRKVL LLKEDHEGLG ISITGGKEHG VPILISEIHP GQPADRCGGL HVGDAILAVN GVNLRDTKHK EAVTILSQQR GEIEFEVVYV APEVDSDDEN VEYEDESGHR YRLYLDELEG GGNPGASCKD TSGEIKVLQG FNKKAVTDTH ENGDLGTASE TPLDDGASKL DDLHTLYHKK SY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
  • 基因功能参考文献：
    1. PIST, a Golgi-associated PDZ domain-containing protein, interacts with PCDH15 in the plasma membrane. PMID: 27867666
    2. CAL can regulate the anterograde trafficking, the internalization as well as the signaling of CRFR1 via modulating the post-translational modifications that the receptor undergoes at the Golgi apparatus. PMID: 26115868
    3. CAL overexpression retards B2AR expression in Golgi apparatus and reduces the receptor expression in the plasma membrane. PMID: 25876703
    4. Six out of 65 (9%) BTC patients were positive for the FIG-ROS1 fusion, comprising two out of 14 (14%) gallbladder carcinoma (GBC) patients and four out of 25 (16%) extrahepatic cholangiocarcinoma (ECC) patients. PMID: 25231053
    5. Our data suggest that PIST contributes to the fine-tuning of b1AR sorting both during biosynthetic and postendocytic trafficking PMID: 25614626
    6. None of 268 gliomas analysed showed the FIG-ROS1(L) rearrangement. PMID: 24999209
    7. Stereochemical preferences modulate affinity and selectivity among CAL PDZ domain protein variants that bind CFTR. PMID: 24210758
    8. FIG-ROS fusion protein, which is found in a subset of intrahepatic cholangiocarcinoma patients, accelerates cholangiocarcinogenesis. PMID: 24154728
    9. Data indicate that E3 ubiquitin ligase MARCH2 co-immunoprecipitated and co-localized with CAL and syntaxin 6 (STX6), and show the ubiquitination of CFTR by MARCH2. PMID: 23818989
    10. The coiled-coil domain of PIST was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.0 A from a crystal belonging to the hexagonal space group P6(2)22 or P6(4)22. PMID: 23545662
    11. crystals of CAL PDZ domain diffracted to 1.4 A resolution PMID: 21543871
    12. establish CAL inhibitors as founding members of a class of CFTR "stabilizers" specifically designed to reduce DeltaF508-CFTR post-endocytic breakdown PMID: 21105033
    13. Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator PMID: 20130090
    14. The FIG-ROS locus encodes for an in-frame fusion protein with a constitutively active kinase activity, suggesting that FIG-ROS may act as an oncogene. PMID: 12661006
    15. CAL retains CFTR in the cell and targets CFTR for degradation. PMID: 14570915
    16. modulates beta1AR intracellular trafficking PMID: 15358775
    17. golgin-160 assosiates in an isoform-specific manner with the Golgi-associated protein PIST PMID: 15951434
    18. intracellular sorting of the somatostatin receptor subtype 5 is regulated by interactions with PDZ domain proteins PIST/GOPC and PDZK1 PMID: 16012170
    19. Our discoveries provide insight into the specific interaction of the GOPC PDZ domain with the C-terminal peptide of Nlg and also provide a general insight about the possible binding mode of the interaction of Nlg with other PDZ domain-containing proteins. PMID: 16882988
    20. CAL is a negative regulator of DeltaF508-CFTR cell-surface expression PMID: 17158866
    21. The cystic fibrosis-associated ligand (CAL) PDZ domain of the C-terminus of cystic fibrosis-associated conductance regulator (CFTR) is competitively involved with PDZ proteins Na+/H+ exchanger-3 regulatory factors NHERF1 and NHERF2. PMID: 18754678

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  • 相关疾病：
    A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • 亚细胞定位：
    Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendrite.
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 17643

    OMIM: 606845

    KEGG: hsa:57120

    STRING: 9606.ENSP00000357484

    UniGene: Hs.191539