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  4. Recombinant Human Eukaryotic translation initiation factor 6 (EIF6)

Recombinant Human Eukaryotic translation initiation factor 6 (EIF6)

  • 中文名称：
    人EIF6重组蛋白
  • 货号：
    CSB-YP007582HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人EIF6重组蛋白
  • 货号：
    CSB-EP007582HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人EIF6重组蛋白
  • 货号：
    CSB-EP007582HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人EIF6重组蛋白
  • 货号：
    CSB-BP007582HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人EIF6重组蛋白
  • 货号：
    CSB-MP007582HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EIF6
  • Uniprot No.：
  • 别名：
    b(2)gcn; B(2)GCN homolog; B4 integrin interactor; Binding protein of beta-4 integrin; CAB; eIF-6; EIF3A; EIF6; Eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; IF6_HUMAN; Integrin beta 4 binding protein; ITGB4BP; OK/SW-cl.27; p27 beta 4 integrin binding protein ; p27(BBP); p27BBP; RP4-614O4.1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-245
  • 氨基酸序列
    MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD TVQIRRVEER LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN DWCAFCGLDT TSTELSVVES VFKLNEAQPS TIATSMRDSL IDSLT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.
  • 基因功能参考文献：
    1. in human CD4(+) T cells, eIF6 levels rapidly increase upon T-cell receptor activation and drive the glycolytic switch and the acquisition of effector functions PMID: 28743432
    2. Studies indicate that eukaryotic translation initiation factor 6 (eIF6) is a central regulator of metabolism independent from mTOR protein and myc proto-oncogene protein (Myc). PMID: 27913676
    3. Propose that eIF6 is necessary for malignant pleural mesothelioma growth. PMID: 26462016
    4. EIF6 plays an important role in controlling cell motility and tumor metastasis. PMID: 25886394
    5. eIF6 is a node regulator of ribosomal function and predict that prioritizing its pharmacological targeting will be of benefit in cancer and Shwachman-Bodian-Diamond syndrome PMID: 25252159
    6. SBDS protein facilitates the release of eIF6, a factor that prevents ribosome joining. PMID: 23115272
    7. eIF6 is one of the downstream effectors of Notch-1 in the pathway that controls cell motility and invasiveness in tumor cell lines PMID: 22348144
    8. The interactions between P311 and ITGB4BP may be very important in the process of tumor cell differentiation and metastasis. PMID: 22365962
    9. The benign prognosis of the Shwachman-Diamond syndrome patients with the int del (20)(q11.21q13.32) may be due to a gene/dosage effect for the EIF6 protein. PMID: 22295858
    10. Inhibiting the induction of two proteins involved in two of the most significantly upregulated cellular processes, ribosome biogenesis (eIF6) and hnRNA splicing (SF3B2/SF3B4), showed that human T cells can enter the cell cycle without growing in size. PMID: 22415777
    11. a direct role for SBDS and EFL1 in catalyzing the translational activation of ribosomes in all eukaryotes, and define SDS as a ribosomopathy caused by uncoupling GTP hydrolysis from eIF6 release PMID: 21536732
    12. P311 and ITGB4BP expression was elevated in non-small cell lung cancer, possibly indicative of a new signaling pathway. PMID: 21029697
    13. Ca(2+)-activated calcineurin phosphatase binds to and promotes nuclear localization of eIF6.Nuclear export of eIF6 is regulated by phosphorylation at Ser-174 and Ser-175 by the nuclear isoform of CK1. PMID: 21084295
    14. eIF6 release regulates ribosome subunit joining and RACK1 provides a physical and functional link between PKC signalling and ribosome activation PMID: 14654845
    15. ITGB4BP is overexpressed in head and neck cancers and its metastases. PMID: 15122657
    16. Human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. PMID: 16530192
    17. results uncover an evolutionarily conserved function of the ribosome anti-association factor eIF6 in miRNA-mediated post-transcriptional silencing PMID: 17507929
    18. Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
    19. definitive evidence that eIF6 and dicer are both upregulated in a significant proportion of ovarian serous carcinomas PMID: 18327211

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  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleolus. Note=Shuttles between cytoplasm and nucleus/nucleolus.
  • 蛋白家族：
    EIF-6 family
  • 组织特异性：
    Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
  • 数据库链接：

    HGNC: 6159

    OMIM: 602912

    KEGG: hsa:3692

    STRING: 9606.ENSP00000363559

    UniGene: Hs.743992