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  4. Recombinant Human E3 ubiquitin-protein ligase TRIM63 (TRIM63)

Recombinant Human E3 ubiquitin-protein ligase TRIM63 (TRIM63)

  • 中文名称：
    人TRIM63重组蛋白
  • 货号：
    CSB-YP846575HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TRIM63重组蛋白
  • 货号：
    CSB-EP846575HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TRIM63重组蛋白
  • 货号：
    CSB-BP846575HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TRIM63重组蛋白
  • 货号：
    CSB-MP846575HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TRIM63
  • Uniprot No.：
  • 别名：
    E3 ubiquitin-protein ligase TRIM63; FLJ32380; IRF; Iris RING finger protein; MURF 1; MURF-1; MuRF1; MURF2; Muscle specific ring finger protein 1; Muscle specific ring finger protein 2; Muscle-specific RING finger protein 1; OTTHUMP00000008701; RING finger protein 28; RNF 28; RNF28; SMRZ; Striated muscle RING zinc finger protein; TRI63_HUMAN; TRIM 63; Trim63; Tripartite motif containing 63; tripartite motif containing 63, E3 ubiquitin protein ligase; Tripartite motif containing protein 63; Tripartite motif-containing protein 63; Ubiquitin ligase TRIM63
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-353
  • 氨基酸序列
    MDYKSSLIQD GNPMENLEKQ LICPICLEMF TKPVVILPCQ HNLCRKCAND IFQAANPYWT SRGSSVSMSG GRFRCPTCRH EVIMDRHGVY GLQRNLLVEN IIDIYKQECS SRPLQKGSHP MCKEHEDEKI NIYCLTCEVP TCSMCKVFGI HKACEVAPLQ SVFQGQKTEL NNCISMLVAG NDRVQTIITQ LEDSRRVTKE NSHQVKEELS QKFDTLYAIL DEKKSELLQR ITQEQEKKLS FIEALIQQYQ EQLDKSTKLV ETAIQSLDEP GGATFLLTAK QLIKSIVEAS KGCQLGKTEQ GFENMDFFTL DLEHIADALR AIDFGTDEEE EEFIEEEDQE EEESTEGKEE GHQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • 基因功能参考文献：
    1. role of the muscle specific E3s MuRF-1 and MAFbx in skeletal muscle wasting during various pathologies, as well as their regulation by modifiable lifestyle factors, were explored (review) PMID: 26738803
    2. the involvement of oxidative stress in the atrophy of COPD peripheral muscle cells in vitro, via the FoxO1/MuRF1/atrogin-1 signaling pathway of the ubiquitin/proteasome system PMID: 27526027
    3. The mitochondrial damage-cGAS-STING-IRF3 pathway is critically involved in metabolic stress-induced endothelial inflammation. PMID: 28302626
    4. Altogether, these results suggest a novel function for p63 as a contributor to muscular atrophic processes via the regulation of multiple genes, including the muscle atrophy gene Trim63. PMID: 26919175
    5. Vitamin D3 might have an inhibitory effect on the expression of MAFbx and MuRF1 in skeletal muscle. PMID: 25876656
    6. MURF1 expression intended to be increased in the skeletal muscle of patients with malignant disease even before cancer related cachexia weight loss. PMID: 25760630
    7. TRIM63 gene expression involved in skin hyperpigmentation. PMID: 25950827
    8. Expression of USP19 correlates with that of MuRF1 and MAFbx/atrogin-1 in skeletal muscles PMID: 26048142
    9. Skeletal muscle atrophy induced by Angiotensin II involves activation of MuRF1 expression. PMID: 26137861
    10. These data strongly supported that rare variants in MuRF1 and MuRF2 are associated with higher penetrance and more severe clinical manifestations of hypertrophic cardiomyopathy. PMID: 24865491
    11. In conclusion, atrogin-1, MuRF1, FOXO1/3A, and eIF3-f mRNA, and protein levels, are differentially regulated by exercise contraction mode but not WPH supplementation combined with hypertrophy-inducing training. PMID: 24458747
    12. Data reveal that Titin protein is a pseudokinase with non-detectable catalytic output but is a high-affinity binding locus for MuRF1. PMID: 24850911
    13. both MuRF1 and MAFbx are enriched in skeletal, cardiac, and smooth muscle--REVIEW PMID: 25096180
    14. SMAD3 regulates transcription of MuRF-1 by increasing FoxO3 binding at a conserved FRE-SBE motif within the proximal promoter region, and by increasing FoxO3 protein content and transcriptional activity. PMID: 24920680
    15. In MuRF1 the COS-box mediates the in vivo targeting of sarcoskeletal structures and points to the pharmacological relevance of the COS domain for treating MuRF1-mediated muscle atrophy. PMID: 24671946
    16. MURF-1 protein gene expression is increased in patients with severe burn injury. PMID: 23816995
    17. Quadriceps muscle MuRF-1 levels did not differ between patients with COPD (with normal or low fat-free mass index) and controls. MURF1 levels were not associated with quadriceps fiber cross-sectional area or strength in patients. PMID: 23844868
    18. Regular exercise training leads to a decrease in Rnf28 expression in skeletal muscle in patients with advanced chronic heart failure. PMID: 22445192
    19. relationship was found between IL-6 and MuRF-1 expression after incubation with PGE2 PMID: 23490068
    20. MuRF-1 RNA expression was significantly increased in malnourished cirrhotic patients vs. well-nourished patients. PMID: 23432902
    21. Data suggest that expression of atrogin-1 and MuRF-1 likely play role in aging-related decrease in muscle mass (i.e., development of sarcopenia); up-regulation of atrogin-1 and MuRF-1 has potential to prevent or reverse sarcopenia. [REVIEW] PMID: 22815045
    22. Human molecular genetic and functional studies identify TRIM63, encoding Muscle RING Finger Protein 1, as a novel gene for human hypertrophic cardiomyopathy. PMID: 22821932
    23. investigation of factors regulating expression of two ubiquitin ligases (MURF1 and MAFbx) in skeletal muscle (i.e., vastus lateralis): effects of resistance exercise and anabolic dietary supplement (i.e., branched-chain amino acids) PMID: 22127230
    24. Data suggest that the inhibition of MuRF1 could be a novel mechanism to prevent or reverse muscle wasting associated with various pathologies. PMID: 21448668
    25. MuRF1 regulates cardiomyocyte cell size and energy metabolism to inhibit cardiac hypertrophy and reverse experimental cardiac hypertrophy. PMID: 21686210
    26. 11beta-HSD1 controls glucocorticoid-induced protein degradation in human and murine skeletal muscle via regulation of the E3 ubiquitin ligases Atrogin-1 and MuRF-1. PMID: 21304964
    27. Reduced expression of MuRF1 and MAFbx in the myocardium might permit hypertrophy characteristic of the early post-infarction remodeling phase. PMID: 19859778
    28. atrogin-1 specifically targets truncated M7t-cMyBP-C, but not WT-cMyBP-C, for proteasomal degradation and that MuRF1 indirectly reduces cMyBP-C levels by regulating the transcription of myosin heavy chain. PMID: 19850579
    29. interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. PMID: 11927605
    30. MURF2 associates transiently with microtubules, myosin and titin during sarcomere assembly. PMID: 12414993
    31. MuRF1 functions as a ubiquitin ligase to catalyze ubiquitylation of troponin I through a RING finger-dependent mechanism PMID: 15601779
    32. MuRF1 mRNA expression was significantly increased in quadriceps of patients with COPD; transcriptional regulation of atrogin-1 and MuRF1 may occur via FoxO-1, but independently of AKT PMID: 17478621
    33. Expression of mRNA for MuRF-1 increased approximately 3-fold at 10 days without changes in MAFbx or tripeptidyl peptidase II mRNA, but all decreased between 10 and 21 days of muscle disuse. PMID: 17901116
    34. MuRF-1 and MAFbx, are differently affected by the exercise as well as by repeated exercise PMID: 17971512
    35. Results showed upregulation of MuRf1 and MAFbx in atrophied muscle and support their role as regulatory peptides in various conditions which lead to muscle atrophy. PMID: 17977773
    36. MuRF1 expression in skeletal muscle re-directs glycogen synthesis to the liver and stimulates pancreatic insulin secretion, providing a feedback loop that connects skeletal muscle metabolism with the liver and the pancreas during metabolic stress. PMID: 18468620
    37. These findings present new insights into the role of the glucocorticoid receptor and FOXO family of transcription factors in the transcriptional regulation of the MuRF1 gene PMID: 18612045
    38. Findings aid the future exploration of the cellular function and therapeutic potential of MuRF1. PMID: 18795805
    39. This study demenostrated that the muscle RING finger 1 protein, human is reduced in skeletal muscle of chronic spinal cord-injured patients. PMID: 19533653

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line.
  • 组织特异性：
    Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • 数据库链接：

    HGNC: 16007

    OMIM: 606131

    KEGG: hsa:84676

    STRING: 9606.ENSP00000363390

    UniGene: Hs.279709