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  4. Recombinant Human Dachshund homolog 1 (DACH1)

Recombinant Human Dachshund homolog 1 (DACH1)

  • 中文名称：
    Recombinant Human Dachshund homolog 1(DACH1),Yeast
  • 货号：
    CSB-YP887059HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Dachshund homolog 1(DACH1),Yeast
  • 货号：
    CSB-EP887059HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Dachshund homolog 1(DACH1),Yeast
  • 货号：
    CSB-EP887059HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Dachshund homolog 1(DACH1),Yeast
  • 货号：
    CSB-BP887059HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Dachshund homolog 1(DACH1),Yeast
  • 货号：
    CSB-MP887059HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DACH1
  • Uniprot No.：
  • 别名：
    DACH; Dach1; DACH1_HUMAN; Dachshund family transcription factor 1; dachshund homolog 1 (Drosophila); Dachshund homolog 1; Dachsund homolog 1; FLJ10138; OTTHUMP00000018494
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-760
  • 氨基酸序列
    MAVPAALIPP TQLVPPQPPI STSASSSGTT TSTSSATSSP APSIGPPASS GPTLFRPEPI ASAAAAAATV TSTGGGGGGG GGGSGGGGGS SGNGGGGGGG GGGSNCNPNL AAASNGSGGG GGGISAGGGV ASSTPINAST GSSSSSSSSS SSSSSSSSSS SSSSSCGPLP GKPVYSTPSP VENTPQNNEC KMVDLRGAKV ASFTVEGCEL ICLPQAFDLF LKHLVGGLHT VYTKLKRLEI TPVVCNVEQV RILRGLGAIQ PGVNRCKLIS RKDFETLYND CTNASSRPGR PPKRTQSVTS PENSHIMPHS VPGLMSPGII PPTGLTAAAA AAAAATNAAI AEAMKVKKIK LEAMSNYHAS NNQHGADSEN GDMNSSVGSS DGSWDKETLP SSPSQGPQAS ITHPRMPGAR SLPLSHPLNH LQQSHLLPNG LELPFMMMPH PLIPVSLPPA SVTMAMSQMN HLSTIANMAA AAQVQSPPSR VETSVIKERV PDSPSPAPSL EEGRRPGSHP SSHRSSSVSS SPARTESSSD RIPVHQNGLS MNQMLMGLSP NVLPGPKEGD LAGHDMGHES KRMHIEKDET PLSTPTARDS LDKLSLTGHG QPLPPGFPSP FLFPDGLSSI ETLLTNIQGL LKVAIDNARA QEKQVQLEKT ELKMDFLRER ELRETLEKQL AMEQKNRAIV QKRLKKEKKA KRKLQEALEF ETKRREQAEQ TLKQAASTDS LRVLNDSLTP EIEADRSGGR TDAERTIQDG RLYLKTTVMY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
  • 基因功能参考文献：
    1. DACH1 expression is decreased in glomerulopathy imply a potential role for DACH1 in the this development of human chornic glomerulopathy. PMID: 27888806
    2. Knockdown of DACH1 expression can remarkably enhance the cell apoptosis, restrain the proliferation, migration and invasion of Capan-1 cells. PMID: 27609579
    3. DACH1 expression regulates the pancreatic cancer cell apoptosis. PMID: 27278537
    4. Low DACH1 expression is associated with invasion of lung adenocarcinoma. PMID: 26810067
    5. This study concludes that MiR-217 is the upstream regulator of PGC-1alpha in breast cancer regulation in vitro, possibly independent of DACH1 signaling pathway. PMID: 27916422
    6. Kaplan-Mier analysis of human NSCLC samples demonstrated that high DACH1 mRNA levels predicted favorable prognosis for relapse-free and overall survival. In agreement, high CXCL5 expression predicted a worse prognosis for survival. PMID: 25788272
    7. DACH1 expression was reduced in hepatocellular carcinoma even at early stage and associated with tumor progression. Overexpression inhibited HCC cell growth and migration by inactivating the Wnt pathway via GSK3beta phosphorylation to suppress beta-catenin. PMID: 25940701
    8. In breast cancer, some GATA3 effects shift from tumor suppressing to tumor promoting during tumorigenesis, with deregulation of three genes, BCL2, DACH1, THSD4, representing major GATA3-controlled processes in cancer progression. PMID: 25410484
    9. Results indicated that DACH1 was a novel molecular marker of RCC and it attributed to the malignant behavior of renal cancer cells. PMID: 25322986
    10. Data indicate that dachshund family transcription factor 1 (DACH1) suppresses gastric cancer cell proliferation, invasion and metastasis by inhibiting transforming growth factor beta (TGF-beta) signalling pathways both in vitro and in vivo. PMID: 24912879
    11. results suggest that DACH1 loss of function results in increased cell growth, motility and invasiveness through TGF-beta-mediated EMT, and DACH1 loss of function has important therapeutic implications for targeted therapies of CRC PMID: 25778865
    12. DACH1 is a determinant of benign and malignant prostate epithelium cellular growth, migration, and cytokine abundance in vivo. PMID: 25769723
    13. Further evaluation of the methylation of DACH1, DKK1, and WIF1 in a clinical patient group confirmed the frequent methylation of WIF1 and intermediate or low frequency of methylation of DACH1 or DKK1, respectively. PMID: 25487617
    14. DACH1 inhibits aldosterone secretion in human adrenals, and transforming growth factor-beta signaling pathway is activated in DACH1 overexpressed cells and may mediate inhibition of aldosterone secretion in human adrenals. PMID: 25776071
    15. Familial young-onset diabetes, pre-diabetes and cardiovascular disease are associated with genetic variants of DACH1 in Chinese PMID: 24465431
    16. Nuclear DACH1 expression appears to be a Luminal A biomarker predictive of good prognosis, but is not independent of clinical stage, tumour size, NPI status or systemic therapy. PMID: 24392136
    17. DACH1 is frequently methylated in human colorectal cancers (CRC) and methylation of DACH1 may serve as detective and prognostic marker in CRC. PMID: 24149323
    18. Cell fate factor DACH1 represses YB-1-mediated oncogenic transcription and translation. PMID: 24335958
    19. DACH1 binds p53 to inhibit NSCLC cellular growth. PMID: 23492369
    20. DACH1 gene promoter methylation could lead to a decrease or absence of DACH1 expression in endometrial carcinoma. PMID: 22781112
    21. the inability of C/EBPalpha and GATA-1 to down-regulate DACH1 expression induced by MLL-AF9 during myeloid differentiation may contribute to t(9;11) leukemogenesis. PMID: 22902925
    22. DACH1 is highly expressed in metastatic ovarian cancer compared with that of normal, benign, and borderline ovarian tissues and could play an important role in cancer growth. PMID: 22367319
    23. The knockdown of DACH1 blocked the cell cycle progression of HL-60 promyeloblastic cells through the decrease of cyclin D1, D3, F, and Cdk 1, 4, and 6 and increase in p21(Cip1), which in turn decreased the phosphorylation of the Rb protein. PMID: 22405764
    24. in cells growing in IGF-1 (and unresponsive to EGF), DACH1 is devoid of tumor suppressor activity. PMID: 21558809
    25. DACH1 is a distinctive tumor suppressor, which not only suppresses growth of tumor cells but also regulates bFGF-mediated tumor-initiating activity of glioma cells PMID: 21750150
    26. DACH1 represses gene transcription through direct DNA binding to the promoter region of target genes by recruiting the transcriptional co-regulator, TCERG1. PMID: 20956529
    27. Forkhead signaling is attenuated by the retinal determination factor DACH1 PMID: 20351289
    28. The altered expression of human DACH1 has been associated with tumor progression and metastasis. DACH1 inhibits breast cancer cellular proliferation via cyclin D1. PMID: 19502783
    29. Findings identify a role of EYA4 and possibly interacting SIX and DACH proteins in MPNSTs and suggest the EYA4 pathway as a rational therapeutic target. PMID: 19901965
    30. forms an alpha/beta structure containing a DNA binding motif similar PMID: 12057194
    31. DACH1 bound to endogenous NCoR and Smad4 in cultured cells; Smad4 was required for DACH1 repression of TGF-beta induction of Smad signaling PMID: 14525983
    32. identifed DACH enhancers in gene deserts PMID: 14563999
    33. The dachshund is required in mushroom body neurons for proper axon guidance and branching in Drosophila melanogaster. PMID: 15818552
    34. DACH1 repressed cyclin D1 through a novel mechanism via a c-Jun DNA-binding partner, requiring the DACH1 alpha-helical DS domain which recruits corepressors to the local chromatin. PMID: 16980615
    35. DACH1 plays an important role in negative regulation of RANKL gene expression in marrow stromal/preosteoblast cells in the bone microenvironment. PMID: 17891780
    36. These data identify a pathway by which an endogenous cell-fate determination factor blocks oncogene-dependent tumor metastasis via a key heterotypic mediator. PMID: 18467491
    37. DACH1 is hypermethylated in renal cell carcinoma. PMID: 18639284
    38. a statistical significance level for DACH1 in gastric cancer PMID: 18957060
    39. DACH1 inhibited ligand-dependent activity of AR mutations identified in patients with androgen-insensitive prostate cancer. The DS domain was sufficient for repression of the AR wild-type but failed to repress an AR acetylation site point mutant. PMID: 19351840
    40. Data show that DACH1 expression, which is lost in poor prognosis human breast cancer, functions as an endogenous inhibitor of ERalpha function. PMID: 19605405

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DACH/dachshund family
  • 组织特异性：
    Widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart. Isoform 4 is found in spleen.
  • 数据库链接：

    HGNC: 2663

    OMIM: 603803

    KEGG: hsa:1602

    UniGene: Hs.129452