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  4. Recombinant Human DNA repair protein complementing XP-A cells (XPA)

Recombinant Human DNA repair protein complementing XP-A cells (XPA)

  • 中文名称：
    人XPA重组蛋白
  • 货号：
    CSB-YP026216HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人XPA重组蛋白
  • 货号：
    CSB-EP026216HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人XPA重组蛋白
  • 货号：
    CSB-EP026216HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人XPA重组蛋白
  • 货号：
    CSB-BP026216HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人XPA重组蛋白
  • 货号：
    CSB-MP026216HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DNA repair protein complementing XP A cells; DNA repair protein complementing XP-A cells; DNA repair protein complementing XPA cells; Excision repair controlling; Xeroderma pigmentosum 1; Xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A complementing protein; Xeroderma pigmentosum group A-complementing protein; XP 1; XP1; xpa; XPA_HUMAN; Xpac
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-273
  • 氨基酸序列
    AAADGALPE AAALEQPAEL PASVRASIER KRQRALMLRQ ARLAARPYSA TAAAATGGMA NVKAAPKIID TGGGFILEEE EEEEQKIGKV VHQPGPVMEF DYVICEECGK EFMDSYLMNH FDLPTCDNCR DADDKHKLIT KTEAKQEYLL KDCDLEKREP PLKFIVKKNP HHSQWGDMKL YLKLQIVKRS LEVWGSQEAL EEAKEVRQEN REKMKQKKFD KKVKELRRAV RSSVWKRETI VHQHEYGPEE NLEDDMYRKT CTMCGHELTY EKM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
  • 基因功能参考文献：
    1. It provides evidence for an H4K20me2-dependent mechanism of XPA recruitment during lesion recognition in the global-genomic branch of NER. PMID: 29233865
    2. This study suggested that the XPA gene rs1800975 Polymorphism was associated with lung cancer susceptibility. By performing multiple separate pairwise comparisons, carriers with AG genotype under the codominant genetic model (AG vs. AA) might play actually the leading role in associating with lung cancer susceptibility in overall and in Asians. PMID: 27460688
    3. We have suggested that the nuclear localization signal on XPA is masked, perhaps in a complex with a cytosolic XPA sequestration protein, and that this complex is disrupted by methanol fixation in non-stressed cells and as part of the DNA damage response following UV irradiation PMID: 29078114
    4. It can be concluded that A23G XPA polymorphism might contribute to increased lung cancer risk in North Indian population emphasizing on poor survival among females PMID: 29516319
    5. molecular analysis of the 5 other exons of the XPA gene, showed that the 2 negative siblings carried no mutation in the XPA gene. This finding suggests that c.682C>T (p.Arg228X) mutation is relatively associated with moderate phenotype in XP group A Moroccan families PMID: 29208038
    6. XPA rs1800975 polymorphism may decrease the risk of breast cancer in both non-Asians and population-based patients PMID: 29953005
    7. Our results indicate that increased XP-1 levels were associated with PCOS after adjustment for potential confounders, which has been shown to be effective in the function of the insulin signaling pathway. PMID: 28831586
    8. data obtained allow us to suggest that XPA can be involved in the post-incision NER stages via its interaction with RPA PMID: 29320546
    9. Data suggest that the multi-protein interactions involved in the nucleotide excision repair (NER) process and potential cytotoxic mechanism associated with Ni(2+) binding in xeroderma pigmentosum complementation group A protein (XPA) may facilitate rational anti-cancer drug design based on the NER mechanism. PMID: 27307058
    10. XPA status does not globally influence human gene transcription. However, XPA significantly influences expression of a small subset of genes important for mitochondrial functions and steroid hormone metabolism. PMID: 28704716
    11. These results suggest that for XPA mutants exhibiting altered DNA-binding properties, a correlation exists between the extent of reduction in DNA-binding affinity and the severity of symptoms in XP patients. PMID: 28860187
    12. These results propose a mechanism for progerin-induced genome instability and accelerated replicative senescence in Hutchinson-Gilford progeria syndrome. PMID: 28515154
    13. These results illustrate that EZH2 may promote carcinogenesis and cancer development of nasopharyngeal carcinoma by transcriptional repression of XPA gene and inactivation of nucleotide excision repair pathway. PMID: 27253463
    14. SNP rs10817938 in promoter region associated with poor prognosis of oral squamous cell carcinoma in Chinese Han population PMID: 27622501
    15. We showed that loss of HERC2 function leads to the accumulation of XPA and BRCA1 in the patient's fibroblasts and generates decreased sensitivity to apoptosis and increased level of DNA repair PMID: 27759030
    16. Accumulating evidence suggests that XPA and the helicase activity of transcription factor IIH (TFIIH) cooperate to verify abnormalities in damaged DNA chemistry. (Review) PMID: 27264556
    17. XPA is a key scaffold protein for human nucleotide excision repair. (Review) PMID: 27247238
    18. XPA reduction increased cell viability of a bladder cancer cell line RT4, while XPA re-expression decreased the cell viability of RT4 cells. Study suggests that downregulated XPA may promote carcinogenesis of bladder cancer via impairment of DNA repair. PMID: 28222669
    19. The risk of esophageal squamous cell carcinoma associated with XPA rs-1800975 was determined. A high ESCC risk was found in subjects who carried the homozygous minor allele of XPA, especially in smokers, those in adobe houses, drinkers of salt tea, or those with a family history of cancer. Variant genotypes of both XPA and XPC in combination showed an increased risk towards ESCC. PMID: 26831662
    20. The role of conformational selection in the molecular recognition of the wild type and mutants XPA67-80 peptides by ERCC1 has been described. PMID: 25973722
    21. We discovered four new XP-A patients and a novel XPA mutation resulting in two diverse patient alleles. PMID: 25393472
    22. a specific XPA genotype is associated with mild phenotype in Xeroderma Pigmentosum in United Kingdom immigrants from India, Pakistan, and Afghanistan PMID: 26743599
    23. Findings suggest that xeroderma pigmentosum complementation group-A (XPA) levels may be a potential predictor of prognosis in locally advanced nasopharyngeal carcinoma (NPC) patients treated with platinum-based chemoradiotherapy. PMID: 26156020
    24. Upon UVC radiation, Nlp interacts with XPA and ERCC1, and enhances their association PMID: 26805762
    25. Data suggest an association of high mobility group box 1 (HMGB1) with xeroderma pigmentosum complementation group A (XPA) protein facilitates the formation of a favorable architectural environment for DNA interstrand crosslinks (ICLs) repair processing. PMID: 26578599
    26. The lack of XPA significantly enhanced the mutant proportion of tandem 8-oxoG in the transcribed strand (12%) compared with that in TSCER122 cells (7.4%) but not in the non-transcribed strand. PMID: 26559182
    27. Xeroderma pigmentosum syndrome is rarely associated with inherited disease-causing XPA mutations in the Brazilian population. PMID: 25913378
    28. This study identified 13 novel mutations and extended the mutation spectrum of XP in the Chinese Han population PMID: 25256075
    29. A tripartite lesion verification mechanism involving XPC, TFIIH, and XPA is necessary for efficient nucleotide excision repair. PMID: 26384665
    30. Homozygous mutations in the XPA gene were seen in xeroderma pigmentosum patients with moderate to severe mental retardation (6/10 families) but not in those without neurological features. PMID: 25566891
    31. Data indicate that residues lysine K221, K222, K224 and K236 in the C-terminal domain of XPA (xeroderma pigmentosum group A) protein are involved in DNA binding. PMID: 25385088
    32. structure of DNA-binding domain of human XPA PMID: 25056193
    33. by detecting the SNPs in blood cells, XPA A23G polymorphic variants might be a promising biomarker in predicting a favor prognosis of NSCLC patients and be helpful towards designing individualized treatments. PMID: 23617284
    34. As RASSF1A modulates both apoptotic DDR and XPA-mediated DNA repair, it may coordinate the balance between repair and death after DNA damage PMID: 25368379
    35. XPA single nucleotide polymorphism and gene frequency in Russians, Tatars and Bashkirs. PMID: 25474887
    36. The XRCC1 rs25487 polymorphism and the XPA rs1800975 polymorphism might exert both independent and interactive effects on the development of breast cancer among northern Chinese women. PMID: 24642895
    37. XPA and PARP1 regulate each other in a reciprocal and poly(ADP-ribose)-dependent manner. PMID: 24953096
    38. Our study suggested that XPA rs1800975 polymorphism might associate with lung cancer risk in overall and in East Asians. PMID: 24696258
    39. Loss of Ser435 within ATR prevents PKA-mediated ATR phosphorylation, disrupts ATR-XPA binding, delays recruitment of XPA to UV-damaged DNA, and elevates UV-induced mutagenesis. PMID: 24950377
    40. Mutation analysis of the candidate XP gene revealed a novel deletion mutation (c.654del A) in exon 5 of XPA gene, which causes frameshift, pre-maturely terminating protein and resulting into a truncated product of 222 amino acid residues instead of 273. PMID: 24063568
    41. Homology modelling corroborates the very close similarity between hydra and human XPA. PMID: 24083246
    42. ATR-mediated phosphorylation induces XPA stabilization by antagonizing HERC2-catalyzed XPA ubiquitination. PMID: 23178497
    43. Transcriptionally active p73 induces translocation of xeroderma pigmentosum group A during apoptosis caused by melphalan. PMID: 24249729
    44. upon DNA damage transport adaptor importin-alpha4 imports XPA into the nucleus in an ATR-dependent manner. PMID: 23861882
    45. report the identification of one novel XPA gene mutation and two known mutations in four unrelated Egyptian families with Xermoderma pigmentosum PMID: 24135642
    46. structure and dynamics of the XPA binding motif in ERCC1 PMID: 23746523
    47. XPA SNPs do not appear to play a major role in lymphoma susceptibility in a Spanish population. PMID: 23818366
    48. XPA genetic polymorphism is associated with esophageal squamous cell carcinoma. PMID: 22941172
    49. We determined the age of the most recent common ancestor of the XPA founder mutation to be 120 generations in Japanese. PMID: 23194742
    50. analysis of novel inhibitors that block XPA-ERCC1 binding PMID: 23272099

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  • 相关疾病：
    Xeroderma pigmentosum complementation group A (XP-A)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    XPA family
  • 组织特异性：
    Expressed in various cell lines and in skin fibroblasts.
  • 数据库链接：

    HGNC: 12814

    OMIM: 278700

    KEGG: hsa:7507

    STRING: 9606.ENSP00000364270

    UniGene: Hs.654364