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  4. Recombinant Human DNA repair protein RAD52 homolog (RAD52)

Recombinant Human DNA repair protein RAD52 homolog (RAD52)

  • 中文名称：
    人RAD52重组蛋白
  • 货号：
    CSB-YP019269HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RAD52重组蛋白
  • 货号：
    CSB-EP019269HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RAD52重组蛋白
  • 货号：
    CSB-EP019269HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RAD52重组蛋白
  • 货号：
    CSB-BP019269HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RAD52重组蛋白
  • 货号：
    CSB-MP019269HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD52
  • Uniprot No.：
  • 别名：
    DNA repair protein RAD52 ; DNA repair protein RAD52; DNA repair protein RAD52 homolog; RAD 52; Rad52; RAD52 homolog (S. cerevisiae) ; RAD52 homolog (S. cerevisiae); RAD52 homolog; RAD52_HUMAN; Recombination protein RAD52 ; Recombination protein RAD52; Rhabdomyosarcoma antigen MU RMS 40.23
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-418
  • 氨基酸序列
    MSGTEEAILG GRDSHPAAGG GSVLCFGQCQ YTAEEYQAIQ KALRQRLGPE YISSRMAGGG QKVCYIEGHR VINLANEMFG YNGWAHSITQ QNVDFVDLNN GKFYVGVCAF VRVQLKDGSY HEDVGYGVSE GLKSKALSLE KARKEAVTDG LKRALRSFGN ALGNCILDKD YLRSLNKLPR QLPLEVDLTK AKRQDLEPSV EEARYNSCRP NMALGHPQLQ QVTSPSRPSH AVIPADQDCS SRSLSSSAVE SEATHQRKLR QKQLQQQFRE RMEKQQVRVS TPSAEKSEAA PPAPPVTHST PVTVSEPLLE KDFLAGVTQE LIKTLEDNSE KWAVTPDAGD GVVKPSSRAD PAQTSDTLAL NNQMVTQNRT PHSVCHQKPQ AKSGSWDLQT YSADQRTTGN WESHRKSQDM KKRKYDPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
  • 基因功能参考文献：
    1. Rad52 competes with Ku for binding to S-region double-strand DNA breaks (DSB) free ends, where it facilitates a DSB synaptic process, which favours intra-S region recombination. PMID: 28176781
    2. Due to its similarity to RAD52, we hypothesized that RDM1 potentially repairs DNA doublestrand breaks arising through DNA replication. PMID: 29845285
    3. The mechanism by which RAD52 depletion causes synthetic lethality in BRCA1 mutant cancer cells depends on the 5' endonuclease EEPD1, which normally functions to cleave stressed replication forks to initiate HR repair. PMID: 29145865
    4. inhibition of ataxia telangiectasia mutated (ATM) protein by siRNA or inhibitor treatment demonstrated that the acetylation of RAD52 at DSB sites is dependent on the ATM protein kinase activity, through the formation of RAD52, p300/CBP, SIRT2, and SIRT3 foci at DSB sites PMID: 29590107
    5. n a cohort of patients with typical symptoms of ischemic heart disease, a common single nucleotide polymorphism of the human RAD52 gene has a role in increased hazard of death, showing that it may influence aging PMID: 29024686
    6. RAD52 gene polymorphism is associated with colorectal cancer. PMID: 26735576
    7. our study demonstrated that RAD52 polymorphisms were associated with colorectal cancer in a Chinese Han cohort PMID: 29245274
    8. DNA-bound RAD52 is efficient at capturing ssDNA in trans. PMID: 28329678
    9. Structure of the human DNA-repair protein RAD52 containing surface mutations has been reported. PMID: 27487923
    10. Rad52 inverse strand exchange plays an important role in RNA-templated double strand break repair in vivo. PMID: 28602639
    11. The mitotic DNA synthesis is RAD52 dependent, and RAD52 is required for the timely recruitment of MUS81 and POLD3 to common fragile sites in early mitosis. PMID: 27984745
    12. Human RAD52-null cells retain a significant level of single-strand annealing (SSA) activity demonstrating perforce that additional SSA-like activities must exist in human cells. Moreover, the SSA activity associated with RAD52 is involved in, but not absolutely required for, most homology-directed repair (HDR) subpathways. Specifically, a deficiency in RAD52 impaired the repair of DNA DSBs. PMID: 28549257
    13. The C-terminal region of yRad52, but not of hRAD52, is involved in ssDNA annealing. This suggests that the second DNA binding site is required for the efficient ssDNA annealing by yRad52. We propose an updated model of Rad52-mediated ssDNA annealing. PMID: 27362509
    14. Study discovered two cis-expression quantitative trait loci SNPs in the RAD52 gene that are associated with its expression and are also associated with lung squamous cell carcinomas (LUSC) risk. PMID: 26013599
    15. these results implicate increased RAD52 expression in both genetic susceptibility and tumorigenesis of upper aerodigestive tract and lung squamous cell carcinoma tumors. PMID: 25793373
    16. RAD52 rs7963551 single nucleotide polymorphism was significantly associated with glioma risk. PMID: 25012956
    17. This study found that only the RAD52 rs7963551 single nucleotide polymorphism was significantly associated with hepatitis B virus related - hepatocellular carcinoma risk. PMID: 24729511
    18. Our findings reveal a novel RAD52/MUS81-dependent mechanism that promotes cell viability and genome integrity in checkpoint-deficient cells, and disclose the involvement of MUS81 to multiple processes after replication stress PMID: 24204313
    19. Our data did not show association between LIG4 and RAD52 SNPs and SLE, its clinical manifestations or ethnicity in the tested population. PMID: 24415301
    20. RAD52 mutation is associated with leukemia. PMID: 23836560
    21. RAD52 is an alternative repair pathway of RAD51-mediated homologous recombination, and a target for therapy in cells deficient in the BRCA1-PALB2-BRCA2 repair pathway. PMID: 22964643
    22. Single nucleotide polymorphisms in RAD52 are associated with myelodysplastic syndromes. PMID: 23339595
    23. Both RAD52 variants and protein expression can predict platinum resistance, and RAD52 variants appeared to predict prognosis in cervical cancer patients. PMID: 23209746
    24. rs7963551 located at the hsa-let-7 binding site may alter expression of RAD52 and contribute to the development of breast cancer in Chinese women. PMID: 23188672
    25. The recruitment kinetics of Rad52 is slower than that of Mdc1, but exhibits the same dependence on LET PMID: 22860035
    26. Silencing of the Rad52 gene in fractionated group of A549 cells made the cells radiosensitive. PMID: 22001234
    27. RAD52(Y104pCMF) specifically targets and wraps ssDNA. Phosphorylation at Y104 enhances ssDNA annealing activity of RAD52 by attenuating dsDNA binding. PMID: 21804533
    28. Rad52 can respond to DNA double-strand breaks and replication stalling independently of BRCA2 PMID: 21148102
    29. the possibility of sumoylation playing an important role in the nuclear transport of RAD52 PMID: 20190268
    30. This study demonstrated a positive correlation between molecular beacon fluorescence intensity, RAD52 gene expression and both gamma ionising radiation and antineoplastic concentration in human TK6 cells. PMID: 19799994
    31. hRad52 stably binds and wraps both, protein free and replication protein A-coated ssDNA. PMID: 20081207
    32. differential effects of Rad52p overexpression on gene targeting and extrachromosomal homologous recombination in a human tumor cell line PMID: 11809887
    33. Analysis of the human replication protein A:Rad52 complex: evidence for crosstalk between RPA32, RPA70, Rad52 and DNA PMID: 12139939
    34. Crystal structure of the homologous-pairing domain from the human Rad52 recombinase in the undecameric form. PMID: 12191481
    35. crystal structure of the single-strand annealing domain PMID: 12370410
    36. RAD52 may play a role in transcription regulation and in targeting DNA damage on transcription active loci to recombinational repair PMID: 12372413
    37. women with Ser346ter nonsense polymorphism of RAD52 not at increased risk of breast cancer in case-control study PMID: 12376524
    38. coordinated WRN and RAD52 activities are involved in replication fork rescue after DNA damage PMID: 12750383
    39. Rad52 facilitates homologous recognition between single-stranded DNA and duplex-DNA through a process that involves unwinding or transient unpairing of the interacting duplex via a novel three-stranded intermediate that does not lead to strand exchange. PMID: 14690434
    40. the ternary complex of hRad52 and XPF/ERCC1 is the active species that processes recombination intermediates generated during the repair of DNA double strand breaks and in homology-dependent gene targeting events PMID: 14734547
    41. For both yeast Rad52 and HsRad52, the yield of strand-exchange reactions was proportional to the fractional A.T content of the DNA substrates, but both enzymes catalyzed exchange with substrates that contained up to at least 50% G.C PMID: 15205482
    42. formation of a stoichiometric complex between HsRad52 and single-stranded DNA was found to be critical for strand exchange activity PMID: 15205484
    43. analysis of residues important for DNA binding in the full-length human Rad52 protein PMID: 15571718
    44. RAD52 Y415X polymorphism is not associated with epithelial ovarian cancer in Australian women PMID: 15670896
    45. Interestingly, presence of hRad52 restores the ability of hRad51 binding to such DNA targets as well. PMID: 16018971
    46. Rad52 protein functions by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break. PMID: 17040915
    47. Data show that DNA repair synthesis, catalyzed by human DNA polymerase eta (poleta) acting upon the priming strand of a D loop, leads to capture and annealing of the second end of a resected DSB in reactions mediated by RAD52 protein. PMID: 18313388
    48. Rad52 aligns two recombining DNA molecules within the first and second DNA binding sites to stimulate the homology search and strand invasion processes. PMID: 18593704
    49. model for hRad52-mediated DNA annealing where ssDNA release and dsDNA zippering are coordinated through successive rearrangement of overlapping nucleoprotein complexes PMID: 19074292
    50. Of the 21 loci screened, RAD52 2259 and RAD52 GLN221GLU may be of importance to disease process and may be associated with papillary thyroid cancer risk in Saudi Arabian population. PMID: 19092295

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAD52 family
  • 数据库链接：

    HGNC: 9824

    OMIM: 600392

    KEGG: hsa:5893

    STRING: 9606.ENSP00000351284

    UniGene: Hs.410355