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Recombinant Human Acylamino-acid-releasing enzyme (APEH)

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  • 中文名称：
    Recombinant Human Acylamino-acid-releasing enzyme(APEH)
  • 货号：
    CSB-EP001899HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    APEH
  • Uniprot No.：
  • 别名：
    AARE; ACPH; ACPH_HUMAN; Acyl peptide hydrolase; Acyl-peptide hydrolase; Acylamino acid releasing enzyme; Acylamino-acid-releasing enzyme; Acylaminoacyl peptidase; Acylaminoacyl-peptidase; APEH; APH; N acylaminoacyl peptide hydrolase; OPH; Oxidized protein hydrolase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    85.2 kDa
  • 表达区域：
    1-732aa
  • 氨基酸序列
    MERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAFWAPGDAGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLIPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCSLLPLGCWSADSQRVVFDSAQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQEEHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDWCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWLRTHLGS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis tag sequence into the targeted gene encoding the 1-732aa of the human APEH. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant human APEH protein carrying N-terminal 6xHis tag. The SDS-PAGE assayed the purity of this recombinant APEH protein greater than 85%. This APEH protein migrated along the gel to a band of about 90 kDa molecular weight.

    APEH is a gene providing instruction of making a protein called acylamino-acid-releasing enzyme in human and belongs to peptidase S9C family. This protein, also abbreviated APH or AARE, is a tetrameric serine protease which specifically removes acetyl amino acids from N-terminally acetylated peptides and play an important role in destroying oxidatively-damaged proteins in living cells. APH preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Deletions of this gene locus are found in various types of carcinomas, including small-cell lung carcinoma and renal cell carcinoma.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

我们一直试图使用AANA作为底物来验证其活性(在405 nm处产生并跟踪硝基苯胺黄色产物),但没有成功。我希望你能给我们一些建议。 我们将蛋白溶解在100ul去离子水中(1ug/uL)。我们在10 mM TRIS (pH = 8.0)和5 mM AANA(总体积200uL)中使用1、2和4ug蛋白质，在37℃下孵育。我们没有看到任何黄色产物，但我们的Hela细胞裂解物对照有。我们之前发布过这个:https://www . science direct . com/science/article/pii/s 0009279721002775 如果你对我们如何测试这种蛋白的活性有什么建议，请告诉我——我们想用它进行动力学分析。

A:
CSB-EP001899HU 该蛋白我们采用的是包涵体复性得到，虽然复性到了可溶性缓冲（不含有变性剂）但是也不能保证复性后的蛋白一定具有活性； 您可以尝试下用APEH蛋白和LGALS8蛋白(CSB-EP012894HU) 进行功能性Elisa验证，看下蛋白是否有活性。

靶点详情

  • 功能：
    This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
  • 基因功能参考文献：
    1. Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
    2. APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
    3. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase S9C family
  • 数据库链接：

    HGNC: 586

    OMIM: 102645

    KEGG: hsa:327

    STRING: 9606.ENSP00000296456

    UniGene: Hs.517969