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  4. Recombinant Helicobacter pylori Thymidylate synthase thyX (thyX)

Recombinant Helicobacter pylori Thymidylate synthase thyX (thyX)

  • 中文名称：
    幽门螺旋杆菌thyX重组蛋白
  • 货号：
    CSB-YP515902HUV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    幽门螺旋杆菌thyX重组蛋白
  • 货号：
    CSB-EP515902HUV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    幽门螺旋杆菌thyX重组蛋白
  • 货号：
    CSB-EP515902HUV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    幽门螺旋杆菌thyX重组蛋白
  • 货号：
    CSB-BP515902HUV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    幽门螺旋杆菌thyX重组蛋白
  • 货号：
    CSB-MP515902HUV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    thyX
  • Uniprot No.：
  • 别名：
    thyX; HP_1533Flavin-dependent thymidylate synthase; FDTS; EC 2.1.1.148; FAD-dependent thymidylate synthase; Thymidylate synthase ThyX; TS; TSase
  • 种属：
    Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-208
  • 氨基酸序列
    MEVICKHYTP LDIASQAIRT CWQSFEYSDD GGCKDKELIH RVGNIFRHSS TLEHLYYNFE IKGLSRGALQ ELSRHRIASL SVKSSRYTLR ELKEVESFLP LNETNLERAK EFLVFVDNEK VNAMSVLALE NLRILLSEHN IKNDLAKYAM PESYKTHLAY SINARSLQNF LTLRSSNKAL KEMQDLAKAL FDALPGEHQY LFEDCLKH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NAD(P)H and FADH(2) as the reductant.
  • 基因功能参考文献：
    1. Report the crystal structure of ThyX from H. pylori strain 26695 in complex with co-factor FAD and substrate dUMP at 2.5 A resolution. It consists of a 1.5 tetramer of ThyX with a total 1248 residues, six FAD and six dUMP molecules in an asymmetric unit. PMID: 22512654
    2. Cloned and purified the ThyX enzyme from H. pylori SS1 strain. Recombinant HpThyX showed maximum activity at pH 8.5, and K(m) values of the two substrates dUMP and CH(2) H(4) folate were determined to be 15.3 +/- 1.25 muM and 0.35 +/- 0.18 mM, respectively. PMID: 21633987
    3. Helicobacter pylori ThyX was purified and crystallized in complex with flavin adenine dinucleotide (FAD) and a diffraction data set was collected to 2.5 A resolution. PMID: 20445247
  • 蛋白家族：
    Thymidylate synthase ThyX family
  • 数据库链接：

    KEGG: heo:C694_07940

    STRING: 85962.HP1533