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  4. Recombinant Escherichia coli Signal peptidase I (lepB), partial

Recombinant Escherichia coli Signal peptidase I (lepB), partial

In Stock
  • 中文名称：
    大肠杆菌lepB重组蛋白
  • 货号：
    CSB-EP360447ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    lepB
  • Uniprot No.：
  • 别名：
    lepB; b2568; JW2552; Signal peptidase I; SPase I; EC 3.4.21.89; Leader peptidase I
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    43.7kDa
  • 表达区域：
    78-324aa
  • 氨基酸序列
    RSFIYEPFQIPSGSMMPTLLIGDFILVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDPVSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETKENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNRDNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. LepB promote the association of the central domain of colicin D with the inner membrane and translocation of the toxic tRNase domain into the cytoplasm. PMID: 26499796
    2. Ile-144 and Ile-86 contribute to the signal peptidase substrate specificity and Ile-144 is important for the accuracy of the cleavage reaction PMID: 15598653
    3. Ile-144 and Ile-86 are key P3 substrate specificity determinants for signal peptidase I PMID: 17077081
    4. A thorough investigation of different roles of Escherichia coli type I signal peptidase residues binding to lipopeptide inhibitor has been performed by a combination of computational alanine scanning mutagenesis and free energy decomposition methods. PMID: 17532654
  • 亚细胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Peptidase S26 family
  • 数据库链接：

    KEGG: ecj:JW2552

    STRING: 316385.ECDH10B_2736