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  4. Recombinant Escherichia coli Protein TolB (tolB)

Recombinant Escherichia coli Protein TolB (tolB)

  • 中文名称：
    大肠杆菌tolB重组蛋白
  • 货号：
    CSB-YP364110ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌tolB重组蛋白
  • 货号：
    CSB-EP364110ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌tolB重组蛋白
  • 货号：
    CSB-EP364110ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌tolB重组蛋白
  • 货号：
    CSB-BP364110ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌tolB重组蛋白
  • 货号：
    CSB-MP364110ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tolB
  • Uniprot No.：
  • 别名：
    tolB; b0740; JW5100Tol-Pal system protein TolB
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-430
  • 氨基酸序列
    EVRIVIDSG VDSGRPIGVV PFQWAGPGAA PEDIGGIVAA DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV VGQVTPNPDG SYNVAYQLVD TGGAPGTVLA QNSYKVNKQW LRYAGHTASD EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY ELRVSDYDGY NQFVVHRSPQ PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA SFPRHNGAPA FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS SNGGQQHIAK QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV LNLVSTDGRF KARLPATDGQ VKFPAWSPYL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. TolB occupies a key intermediary position in the Tol-Pal system because it communicates directly with both membrane-embedded components, Pal in the outer membrane and TolA in the inner membrane. The Tol-Pal system is also required for polar localization of chemoreceptors clusters. The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity. Is involved in the uptake of some colicins A.
  • 基因功能参考文献：
    1. Colicin N and M13 phage protein G3p compete with TolB for a binding site on TolA. PMID: 25536997
    2. The N-terminal 107 residues of ColA (TA1-107) bound to TolB to show that the TolB box of ColA consists of a 12-residue domain that binds to the beta-propeller canyon of TolB. PMID: 19627502
    3. the TolB box sequence in ColE9 is actually larger than the pentapeptide and extends from residues 34 to 46 PMID: 16166536
    4. Results identify the 16-residue TolB binding epitope in the natively disordered T-domain of the nuclease colicin E9 (ColE9) and present the crystal structure of the complex. PMID: 16894158
    5. analysis of allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins PMID: 19696740

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  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    TolB family
  • 数据库链接：

    KEGG: ecj:JW5100

    STRING: 316385.ECDH10B_0807