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  4. Recombinant Escherichia coli Maltose/maltodextrin-binding periplasmic protein (malE)

Recombinant Escherichia coli Maltose/maltodextrin-binding periplasmic protein (malE)

  • 中文名称：
    大肠杆菌malE重组蛋白
  • 货号：
    CSB-YP360183ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌malE重组蛋白
  • 货号：
    CSB-EP360183ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌malE重组蛋白
  • 货号：
    CSB-BP360183ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌malE重组蛋白
  • 货号：
    CSB-MP360183ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    malE
  • Uniprot No.：
  • 别名：
    malE; b4034; JW3994; Maltose/maltodextrin-binding periplasmic protein; MMBP; Maltodextrin-binding protein; Maltose-binding protein; MBP
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-396
  • 氨基酸序列
    KIEE GKLVIWINGD KGYNGLAEVG KKFEKDTGIK VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS LIYNKDLLPN PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTRITK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.
  • 基因功能参考文献：
    1. Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-like folding intermediate that brings together sequentially remote segments and then folds globally after a long delay. PMID: 29295923
    2. Our current understanding of what makes MBP such an effective solubility enhancer for other proteins and how it facilitates the proper folding of its fusion partners is presented [REVIEW] PMID: 28132493
    3. Maltose Binding Protein is key to the coupling of maltose transport to ATP hydrolysis in vivo, because it facilitates the progression of the MalK dimer from the open to the semi-open conformation, from which it can proceed to hydrolyze ATP PMID: 26268698
    4. activates mouse macrophages and polarizes them into M1 macrophages PMID: 24825603
    5. a salt-bridge motif in the maltose-binding protein has a role in the initial steps of substrate transport PMID: 16143635
    6. Our findings indicate that the solubility-enhancing activity of MBP is mediated by its open conformation and point to a likely role for the ligand-binding cleft in the mechanism of solubility enhancement. PMID: 17964542
    7. These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB. PMID: 18241889
    8. tat mutants are able to transport maltose in the absence of malE. PMID: 18385983

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  • 亚细胞定位：
    Periplasm.
  • 蛋白家族：
    Bacterial solute-binding protein 1 family
  • 数据库链接：

    KEGG: ecj:JW3994

    STRING: 316385.ECDH10B_4223