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  4. Recombinant Escherichia coli ATP-dependent DNA helicase recG (recG)

Recombinant Escherichia coli ATP-dependent DNA helicase recG (recG)

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  • 中文名称：
    大肠杆菌recG重组蛋白
  • 货号：
    CSB-EP326126ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    recG
  • Uniprot No.：
  • 别名：
    recG; radC; b3652; JW3627; ATP-dependent DNA helicase RecG; EC 3.6.4.12
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    92.4kDa
  • 表达区域：
    1-693aa
  • 氨基酸序列
    MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAAMKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC.
  • 基因功能参考文献：
    1. results provide mechanistic insights into the modes of interaction of RecG with the replication fork, suggesting a novel role of RecG in the repair of stalled DNA replication forks. PMID: 29432678
    2. Data suggest that RecG and RuvABC catalyze alternative steps in DNA repair/recombination and collaborate to stabilize DNA during repair of double-strand DNA breaks; RecG may prevent DNA amplification at D-loops formed by recombination and at arrested replication forks. (RecG = RecG DNA helicase; Ruv = Holliday junction DNA helicase subunit) [REVIEW] PMID: 28155219
    3. interaction occurs via the wedge domain of RecG and the intrinsically disordered linker (IDL) of SSB, in a manner similar to that of SH3 domains binding to PXXP motif-containing ligands in eukaryotic cells. PMID: 28078722
    4. Once RecG has regressed the fork, it will dissociate leaving the now relaxed, Holliday junction-like DNA, available for further processing by enzymes such as RuvAB. [review] PMID: 25613916
    5. RecG co-localises with sites of DNA replication in Escherichia coli. PMID: 24692661
    6. Abolishing PriB leads to suppression of the recG null phenotype. PMID: 22957744
    7. data are consistent with RecG and ssDNA exonucleases acting to limit PriA-mediated re-replication of the chromosome and the consequent generation of linear DNA branches that provoke recombination and delay chromosome segregation PMID: 20647503
    8. The s show that the extensive DnaA-independent stable DNA replication observed without RecG can lead to replication of any area of the chromosome. PMID: 19818016
    9. allows repair of damaged replication forks to proceed without recourse to potentially mutagenic recombination PMID: 15516585
    10. Data suggest that the processivity of RecG on branched DNA substrates is dependent on the ability of its wedge domain to establish strong binding at the branch point. PMID: 15695524
    11. Inactivation of recG stimulates recombinant formation about five-fold PMID: 16257588
    12. manner and timing of the interaction of RecG with DNA at stalled replication forks PMID: 17292398
    13. RecG decreases the number of replication forks in UV-irradiated cells by limiting UV-induced, DnaA-independent initiation of replication. PMID: 19538444

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  • 蛋白家族：
    Helicase family, RecG subfamily
  • 数据库链接：

    KEGG: ecj:JW3627

    STRING: 316385.ECDH10B_3834