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  4. Recombinant Drosophila melanogaster Polycomb group protein Pc (Pc)

Recombinant Drosophila melanogaster Polycomb group protein Pc (Pc)

  • 货号：
    CSB-YP333127DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP333127DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP333127DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP333127DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP333127DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pc
  • Uniprot No.：
  • 别名：
    Pc; CG7618; Polycomb group protein Pc; Protein polycomb
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-390
  • 氨基酸序列
    MTGRGKGSKG KLGRDNATDD PVDLVYAAEK IIQKRVKKGV VEYRVKWKGW NQRYNTWEPE VNILDRRLID IYEQTNKSSG TPSKRGIKKK EKEPDPEPES EEDEYTFTEN DVDTHQATTS SATHDKESKK EKKHHHHHHH HHHIKSERNS GRRSESPLTH HHHHHHHESK RQRIDHSSSS NSSFTHNSFV PEPDSNSSSS EDQPLIGTKR KAEVLKESGK IGVTIKTSPD GPTIKPQPTQ QVTPSQQQPF QDQQQAEKIA SEAATQLKSE QQATPLATEA INTTPAESGA EEEEVANEEG NQQAPQVPSE NNNIPKPCNN LAINQKQPLT PLSPRALPPR FWLPAKCNIS NRVVITDVTV NLETVTIREC KTERGFFRER DMKGDSSPVA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Component of the PcG multiprotein PRC1 complex, a complex that acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-118', rendering chromatin heritably changed in its expressibility. Promotes locus-specific chromatin compaction.
  • 基因功能参考文献：
    1. we demonstrate that the evolutionary conserved Lim3 core promoter provides basic Lim3 expression, whereas structural changes in the Lim3 PRE of distal promoter provide stage-, and tissue-specific Lim3 expression. Therefore, we hypothesize that PcG/TrxG proteins, which are directly involved in Lim3 transcription regulation, participate in lifespan control PMID: 29555581
    2. Control of the gene activity by polycomb and trithorax group proteins in Drosophila PMID: 29372960
    3. a Pc-mediated, Hisotne 3 lysine 27 trimethylation-dependent positive transcriptional regulation of Senseless (Sens), a key transcription factor required for development, is reported. PMID: 27002220
    4. PRC1 components act as neoplastic tumor suppressors independently of PRC2 function PMID: 27643538
    5. a novel activity of polycomb: inhibition of the histone acetylation activity of CREB-binding protein, is identified. PMID: 26802126
    6. transcription through the PRE is not sufficient for inducing an epigenetic switch in PRE activity. PMID: 26504232
    7. From the Drosophila melanogaster vestigial (vg) Polycomb response element/Trithorax response element switches the status of the element. PMID: 25108384
    8. This study shows that exposure to G418 stress reduces the maternal levels of Polycomb in the offspring embryos and this reduction contributes to the inheritance of induced expression. PMID: 24535443
    9. CpG island encompassing the promoter and first exon of human DNMT3L gene is a PcG/TrX response element PMID: 24743422
    10. of Polycomb at tTAF-dependent spermatogenesis genes in precursor cells argues against a model where Polycomb displacement is the mechanism of spermatogenesis gene activation PMID: 24146626
    11. These studies reveal a previously unrecognized role for PRC1 in facilitating productive gene transcription and provide new insights into how cohesin and PRC1 control development. PMID: 23818863
    12. Data imply that Trx, Pc, and E(z) remain bound or rapidly rebind to nascent DNA in the absence of trimethylated histones during DNA replication in Drosophila embryos. PMID: 22921915
    13. dHCF possesses Enhancer of TrxG and PcG (ETP) properties. PMID: 22174740
    14. These findings support a model in which Polycomb repressor complexes establish motoneuron-specific competence windows in neuroblasts that transition from motoneuron to interneuron production. PMID: 22219354
    15. Evidence is presented that Polycomb group (PcG) repressors can influence paused Pol II. PMID: 21700228
    16. Chromatin immunoprecipitation (ChIP) profiles of the Polycomb protein and its associated H3K27me3 histone mark reflect this insulator-dependent chromatin conformation. PMID: 21262819
    17. a Polycomb response element with strong Polycomb group binding and repressive activity cannot mediate trans interactions PMID: 21135119
    18. Polycomb and trithorax control genome expression by determining the alternative epigenetic states of chromatin for key developmental regulators PMID: 21254568
    19. CtBP regulates expression of intergenic transcripts that controls DNA binding by PcG proteins and subsequent histone modifications and transcriptional activity. PMID: 20082324
    20. PcG targets are found in a transcriptionally active state characterized by formation of a chromatin domain enriched in features that antagonize PcG repression. PMID: 20062800
    21. Polycomb is involved in transcriptional silencing of transgenic Adh. PMID: 11864605
    22. The Drosophila trithorax protein is a coactivator required to prevent re-establishment of polycomb silencing. PMID: 11973279
    23. Genomic imprinting in Drosophila is maintained by the products of Suppressor of variegation and trithorax group, but not Polycomb group, genes. PMID: 12242505
    24. these studies establish a link between histone methylation and Polycomb group-mediated gene silencing PMID: 12351676
    25. transcription through a Polycomb-Response Element (PRE) interferes with the maintenance of a Polycomb repression complex PMID: 12397100
    26. Batman, together with the GAGA factor, binds to MHS-70, a 70-bp fragment of the bithoraxoid Polycomb response element PMID: 12556479
    27. self-association is functionally important for Polycomb PMID: 12897052
    28. identified several members of the Polycomb and trithorax classes of genes required for the normal pattern of photoreceptor differentiation PMID: 15020417
    29. Polycomb silencing blocks transcription initiation. PMID: 15053881
    30. Data show that multiple pathways of cross-regulation help to explain the large size of the Polycomb group family of genes, but they complicate the genetic analysis of any single member. PMID: 15314179
    31. hedgehog is maintained in a repressed state by the Polycomb group (PcG) chromatin proteins in a tissue-specific manner. PMID: 16024236
    32. findings show that testis-specific TAF (TBP-associated factor) homologs required for terminal differentiation of male germ cells may activate target gene expression in part by counteracting repression by Polycomb PMID: 16272126
    33. Data show that components of the RNA interference (RNAi) machinery are involved in polycomb group-mediated silencing at Fab-7 and in the production of small RNAs at transgenic Fab-7 copies. PMID: 16530043
    34. the spread of methylation from the Polycomb response elements at the Drosophila ubx gene and the silencing effect can be blocked by the gypsy insulator PMID: 16887811
    35. Stufy shows polycomb group proteins bind all major elements including PREs and core promoters to form a topologically complex structure which is to stably maintain alternative transcription states. PMID: 17828248
    36. Polycomb-group (PcG) proteins are highly conserved epigenetic transcriptional repressors that play central roles in numerous examples of developmental gene regulation of Drosophila larva. PMID: 18216170
    37. Association of Trl and Pc with bxd depends on transcriptional activity. PMID: 18550707
    38. Absence of endogenous Ubx transcription when Ubx levels are highly elevated probably activates Polycomb complexes on a Polycomb response element located in the Ubx major intron. This, in turn, brings about permanent repression of Ubx transcription. PMID: 18715947
    39. analysis suggests a dynamic relationship between Pc Polycomb /Pho (Pleiohomeotic) binding and gene transcription. PMID: 18773083
    40. results indicate that DNA binding proteins serve as platforms to assist PcG and trxG binding. Furthermore, several DNA sequence features discriminate between PcG- and TRX-N-bound regions PMID: 19143474

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: dme:Dmel_CG32443

    STRING: 7227.FBpp0078059

    UniGene: Dm.13644