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  4. Recombinant Drosophila melanogaster ATP-dependent RNA helicase WM6 (Hel25E)

Recombinant Drosophila melanogaster ATP-dependent RNA helicase WM6 (Hel25E)

  • 中文名称：
    黑腹果蝇Hel25E重组蛋白
  • 货号：
    CSB-YP636676DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Hel25E重组蛋白
  • 货号：
    CSB-EP636676DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Hel25E重组蛋白
  • 货号：
    CSB-EP636676DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Hel25E重组蛋白
  • 货号：
    CSB-BP636676DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Hel25E重组蛋白
  • 货号：
    CSB-MP636676DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Hel25E
  • Uniprot No.：
  • 别名：
    Hel25E; Dbp25F; hel; WM6; CG7269ATP-dependent RNA helicase WM6; DEAD box protein UAP56; Dmrnahel; EC 3.6.4.13; HEL/UAP56
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-424
  • 氨基酸序列
    MADNDDLLDY EDEEQTETTA VENQEAPKKD VKGTYVSIHS SGFRDFLLKP EILRAIVDCG FEHPSEVQHE CIPQAVLGMD ILCQAKSGMG KTAVFVLATL QQLEPSDNNT CHVLVMCHTR ELAFQISKEY ERFSKYMPTV KVAVFFGGMA IQKDEETLKS GTPHIVVGTP GRILALIRNK KLNLKLLKHF VLDECDKMLE QLDMRRDVQE IFRSTPHGKQ VMMFSATLSK DIRPVCKKFM QDPMEVYVDD EAKLTLHGLQ QHYVNLKENE KNKKLFELLD VLEFNQVVIF VKSVQRCVAL SQLLTEQNFP AIGIHRGMTQ EERLNRYQQF KDFQKRILVA TNLFGRGMDI ERVNIVFNYD MPEDSDTYLH RVARAGRFGT KGLAITFVSD ENDAKILNEV QDRFDVNISE LPEEIDLSTY IEGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for mRNA export out of the nucleus. Probable RNA helicase that may regulate entry into mitosis by down-regulating the expression of other genes whose activity may be rate-limiting for entry into mitosis during embryogenesis. Binds to salivary gland chromosomes and modifies position effect variegation. Promotes an open chromatin structure that favors transcription during development by regulating the spread of heterochromatin.
  • 基因功能参考文献：
    1. Study presents evidence that UAP56 and Vas are cluster transcript binding proteins that organize a piRNA-processing compartment that spans the nuclear envelope. PMID: 23141543
    2. UAP56 RNA helicase is required for axis specification and cytoplasmic mRNA localization. PMID: 18237727
  • 亚细胞定位：
    Nucleus speckle. Note=Locates to nuclei of embryos and ovaries, but disappears in mitotic domains of embryos as chromosomes condense.
  • 蛋白家族：
    DEAD box helicase family, DECD subfamily
  • 数据库链接：

    KEGG: dme:Dmel_CG7269

    STRING: 7227.FBpp0078754

    UniGene: Dm.3332