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  4. Recombinant Cricetulus griseus Cathepsin D (Ctsd), partial

Recombinant Cricetulus griseus Cathepsin D (Ctsd), partial

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  • 中文名称：
    Recombinant Cricetulus griseus Cathepsin D(Ctsd),partial
  • 货号：
    CSB-EP006187DXU
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006187DXU could indicate that this peptide derived from E.coli-expressed Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) CTSD.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006187DXU could indicate that this peptide derived from E.coli-expressed Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) CTSD.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • Uniprot No.：
  • 种属：
    Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    53.3kDa
  • 表达区域：
    65-408aa
  • 氨基酸序列
    GPVSELLKNYLDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSGKSSTFVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSEQPGGLKVEKQIFGEAIKQPGITFIAAKFDGILGMGYPSISVNNVVPVFDNLMQQKLVEKNIFSFFLNRDPTGQPGGELMLGGIDSKYYEGELSYLNVTRKAYWQVHMDQLDVANGLTLCKGGCEAIVDTGTSLLVGPVDEVKELQKAIGAVPLIQGEYMIPCEKVSSLPSVTLKLGGKDYELSPSKYVLKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGTYYTVFDRDNNRVGFAKAATL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis-SUMO tag sequence into the targeted gene encoding the 65-408aa of the Cricetulus griseus CTSD. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant Cricetulus griseus CTSD protein carrying N-terminal 6xHis-SUMO tag. The SDS-PAGE assayed the purity of this recombinant CTSD protein greater than 90%. This CTSD protein migrated along the gel to a band of about 50 kDa molecular weight.

    Cathepsin D is a protein encoding by a gene named H671_3g9701 in chinese hamster or a gene named CTSD in human. Cathepsin D is an acid lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. Cathepsin D can dissolve basement membrane in an acidic environment, degrade extracellular matrix and connective tissue, and is related to aggressive biological behavior. The specificity of cathepsin D is similar topepsin A, but it can not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Studies have confirmed that Cathepsin D plays an important role in tumorigenesis and development.

  • Datasheet & COA：
    Please contact us to get it.

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