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  4. Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1)

Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1)

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  • 中文名称：
    白念珠菌PRA1重组蛋白
  • 货号：
    CSB-YP308545CZD
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP308545CZD could indicate that this peptide derived from Yeast-expressed Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) PRA1.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP308545CZD could indicate that this peptide derived from Yeast-expressed Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) PRA1.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    PRA1
  • Uniprot No.：
  • 别名：
    PRA1; FBP1; CAALFM_C406980WA; CaO19.10623; CaO19.3111; pH-regulated antigen PRA1; 58 kDa fibrinogen-binding mannoprotein
  • 种属：
    Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    33.4 kDa
  • 表达区域：
    16-299aa
  • 氨基酸序列
    APVTVTRFVDASPTGYDWRADWVKGFPIDSSCNATQYNQLSTGLQEAQLLAEHARDHTLRFGSKSPFFRKYFGNETASAEVVGHFDNVVGADKSSILFLCDDLDDKCKNDGWAGYWRGSNHSDQTIICDLSFVTRRYLTQLCSSGYTVSKSKTNIFWAGDLLHRFWHLKSIGQLVIEHYADTYEEVLELAQENSTYAVRNSNSLIYYALDVYAYDVTIPGEGCNGDGTSYKKSDFSSFEDSDSGSDSGASSTASSSHQHTDSNPSATTDANSHCHTHADGEVHC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell surface protein involved in the host-parasite interaction during candidal infection. With MP65, represents a major component of the biofilm matrix. Sequesters zinc from host tissue and mediates leukocyte adhesion and migration. As a surface protein, binds the two human complement regulators CFH and CFHR1, as well as plasminogen PLG, mediates complement evasion and extra-cellular matrix interaction and/or degradation. As a released protein, enhances complement control in direct vicinity of the yeast and thus generates an additional protective layer which controls host complement attack, assisting the fungus in escaping host surveillance. Binds to host fluid-phase C3 and blocks cleavage of C3 to C3a and C3b, leading to inhibition of complement activation. Mediates also human complement control and complement evasion through binding to C4BPA, another human complement inhibitor, as well as through binding to host integrin alpha-M/beta-2. Decreases complement-mediated adhesion, as well as uptake of C.albicans by human macrophages.
  • 基因功能参考文献：
    1. Pra1 is a hierarchical complement inhibitor that targets C3 by cleaving C3 at a unique site. This inhibited effector function of the activation fragments. Pra1 also bound to C3a and C3b generated by human convertases and blocked their effector functions, C3a binding to human C3a receptor, calcium signaling, IL-8 release, C3b deposition, opsonophagocytosis, and killing by neutrophils. PMID: 28860090
    2. surface-exposed Pra1 plays a role in the recognition of Candida albicans, especially hyphal cells, by human neutrophils PMID: 21820180
    3. recognition of pH-regulated antigen 1 protein (Pra1p) by integrin alpha(M)betaplays a pivotal role in determining fungal virulence and host response and protection against C. albicans infection PMID: 21245270
    4. Data show that the fungus secretes a potent complement inhibitor, pH-regulated Ag 1 (Pra1), which in the direct surrounding of the pathogen binds to fluid-phase C3 and blocks cleavage of C3 to C3a and C3b. PMID: 20644161
    5. Therefore, it appears that Pra1p can play at most a minor role in fibrinogen binding to C. albicans. PMID: 18625733
    6. The pH-regulated antigen 1 (Pra1) protein was identified as a novel Factor H and FHL-1 binding protein. PMID: 19850343

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  • 亚细胞定位：
    Secreted. Note=Found primarily on the cell surface of filamentous forms and enriched at hyphal tips.
  • 蛋白家族：
    ZPS1 family
  • 数据库链接：

    KEGG: cal:CAALFM_C406980WA